FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l03n01_heat_1h_rep1_shoot.35100000055c43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l03n01_heat_1h_rep1_shoot.35100000055c43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18358612
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2151.207809E-915.4638910-11
GTCACCA1854.9736627E-815.40397528-29
ACACGTC2101.4739271E-814.70125512-13
GAACTCC2001.2923738E-714.2488720-21
AGAGCAC3255.4630982E-513.1527318
GCTTGAA2204.1210114E-712.95517758-59
CCAGATC2052.6417983E-612.74403932-33
GAGCACA3408.336296E-512.5725339
CACGTCT1751.0929088E-412.21343414-15
CTGCTTG2359.1631773E-712.1282556-57
CCAGTCA2002.8721115E-511.8743526-27
CAGTCAC2653.084915E-711.65030626-27
ACTCCAG2703.930927E-711.43437222-23
CTCGTAT2309.534217E-611.3595942-43
AGTCACC1902.3498945E-411.24895528-29
TCCAGTC2754.9837035E-711.22650524-25
CTCCAGT2054.7443932E-410.42611724-25
TGAACTC2300.00136366579.29274220-21
ATTACCC2400.00200644078.90695142-43
CATCTCG3458.794123E-58.26129538-39
Produced by FastQC (version 0.11.2)