FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n02_salt_24h_rep1_shoot.35200000055c0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n02_salt_24h_rep1_shoot.35200000055c0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22287810
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG1607.0454413E-411.87614838-39
CAGCGGG1500.00587398911.08485394-95
TCGGTGG1952.9842922E-410.96259838-39
AGTGTAG2851.0091991E-49.16831528-29
GAGTGTA2100.00672598749.05226-27
CTCGGTG2200.0099113088.63361236-37
GTAGGGA3501.0344888E-48.14695618-19
GTGTAGG4201.2335686E-57.920722516-17
GTAGATC4150.00558249336.293711730-31
TGTAGAT4950.00538859385.756232330-31
TCGTGTA5450.00249172075.66778514-15
GGGGAAA6100.00158409695.453551320-21
AGCGTCG7204.1053002E-45.278578310-11
Produced by FastQC (version 0.11.2)