FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n02_salt_24h_rep1_root.35200000055c76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n02_salt_24h_rep1_root.35200000055c76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21143223
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGG1401.7812063E-523.7503033
CGGGGTG1205.9117156E-921.78085514-15
GGGGGCC4150.020.0357142-43
AATCGGG1456.133422E-419.6634731
GGGTGGC1303.2773642E-718.27432842-43
ATCGGAG1700.001786903116.7675782
GTGGGGA1306.603246E-616.45038218-19
TCCGGGG1955.5551936E-915.82834736-37
GGGCCGG3650.015.62105644-45
ATCGGGA1850.003136899615.4119121
TCGGGGG3800.014.37642638-39
CGGGGAG1000.00526603114.25655914-15
GCCGGGT1453.070458E-413.10639146-47
TTCCGGG1656.317928E-512.95046436-37
ACTGTGG1300.002014031412.79007694-95
CTCGGGG1801.4244957E-411.87125936-37
GGGGGAA6250.011.78528320-21
GCATTCC7702.1827873E-1111.7209273
GGGGAGA2703.9177576E-711.43734728-29
GGCCGGT3001.2744931E-711.08582346-47