FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n02_ctrl_1h_rep2_root.35200000055bd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n02_ctrl_1h_rep2_root.35200000055bd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45620941
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG6700.068.797511
ATCGGGA9000.052.2584462
ACGGGAG5350.046.1675833
GAGGGGC9450.045.7279629
GAACGGG5000.041.8175351
GGATCGG4850.038.21191
GGACGGG5200.037.4676361
ATGGGAG4300.036.452983
TCGGGGG22850.035.86274738-39
GAGCGGC5000.034.1904459
TCTCGGG6100.033.86226336-37
CTCGGGG9000.033.50331536-37
GAGAGGG14850.032.9244657
GGATGGG7400.032.750221
TGGGAGA5200.031.0473824
TAGGGGA6300.030.92770418-19
TCCGGGG9250.030.30058138-39
CGGGAAG3350.029.7662334
TCGGGAA5650.029.4243683
ATTCGGG5800.029.06410236-37
Produced by FastQC (version 0.11.2)