FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n01_salt_24h_rep1_shoot.35100000055c0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n01_salt_24h_rep1_shoot.35100000055c0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22287810
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC4600.034.0690088
GAACTCC3000.030.86948620-21
CAGTCAC3350.026.23048426-27
GCACACG3750.025.3280710-11
ACACGTC3850.024.67039112-13
CACAGTG3600.024.40782732-33
CTCCAGT3450.024.0927424-25
GATCTCG3950.024.04946738-39
TGATCTC3500.023.7488538-39
CCAGTCA3450.023.40503126-27
AACTCCA3700.023.1043722-23
CACGTCT3750.022.16327914-15
TGAACTC3650.022.11933720-21
AGCACAC4050.019.9341310-11
GCTTGAA4800.019.79879458-59
TCCCTGG600.004762906619.79408590-91
CACACGT4350.019.10537712-13
CTCGTAT5600.014.41972142-43
ATCTCGT6150.013.12981340-41
TCTCGTA7350.012.92490640-41
Produced by FastQC (version 0.11.2)