FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n01_salt_24h_rep1_root.35100000055c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n01_salt_24h_rep1_root.35100000055c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21143223
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTC501.3010595E-633.2486736-37
ACTCCCG404.5000672E-429.68329222-23
GCTTGAA10000.026.84054860-61
CGCACAT9050.026.5044636-37
TCCGCAC10150.025.03508434-35
CCCGTCC500.001654779423.74960930-31
ACCTCTC753.037413E-522.16582938-39
TCTCGTA12850.021.8096542-43
CTCCCGT550.00288142121.58784722-23
CACGTCC12500.020.89965630-31
CGCACCT804.9962502E-520.78041636-37
AACTCCC600.00477044119.78867120-21
CCTCTCG857.963167E-519.55859640-41
CACCTCT857.964759E-519.55808438-39
CCGCACC901.2353505E-418.47084834-35
CAGTCAC14250.017.49958826-27
ATGCCGT17500.016.55753548-49
ACATCTC8850.015.83314740-41
ACTCTAG1206.163109E-515.83108922-23
GAACTCT1105.713351E-415.1113520-21
Produced by FastQC (version 0.11.2)