FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l02n01_ctrl_1h_rep2_root.35100000055bd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l02n01_ctrl_1h_rep2_root.35100000055bd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45620941
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC111600.053.5761078
TCCCGAT71800.043.82765236-37
CGTCCCG71500.043.5101334-35
CCCGTCC70350.043.1753732-33
GATCTCG73350.042.99949340-41
GAGCACA139000.042.98099
CACCCGT72250.042.1407930-31
CCGATCT73350.041.8330238-39
GCTTGAA79250.041.7808460-61
CCCGATC68050.041.39170538-39
GTCACCC73500.041.3904828-29
GTCCCGA72200.040.7559436-37
CGATCTC72600.040.27047740-41
TCTCGTA79350.040.1679142-43
CAGTCAC77450.040.01650626-27
ACCCGTC72400.039.42716632-33
TCACCCG72850.038.76187530-31
CTCGTAT77350.038.28959744-45
AGTCACC75350.037.94738828-29
GAACTCC83500.037.79493720-21
Produced by FastQC (version 0.11.2)