FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l01n02_ctrl_1h_rep1_root.35200000055cc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l01n02_ctrl_1h_rep1_root.35200000055cc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40387281
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGGG1407.121398E-716.97017538-39
TCGGGGG1752.4976544E-816.29136838-39
CCTTGGA2308.0481626E-414.4435737
GGTGGGG2202.7024726E-814.03933718-19
AGGGGCG1200.001103043913.85395610-11
GGGGGGC4700.013.14613142-43
TTCCGGG1659.1494183E-411.51190836-37
GGGGCCC2309.480236E-611.36543844-45
GGAGGGG3150.00850855310.5455136
GTGGGGG2401.8226485E-49.89953318-19
GGGGGCC2502.7423527E-49.50563944-45
GGGGGAA6300.09.43111120-21
AAGGGGG4504.179492E-88.97897526-27
GGGGGTG2654.9151934E-48.96759540-41
GGGTGGG2150.0081646248.84060716-17
CAGGACT2150.0081758368.83912612-13
CTCGGTG5307.55972E-98.51563394-95
TCGGTGG4604.9796145E-67.74591394-95
GGGGGGA6801.0528311E-87.339623520-21
GAAAGGG3903.6054075E-47.31342824-25
Produced by FastQC (version 0.11.2)