FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l01n01_ctrl_1h_rep2_shoot.35100000055cf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l01n01_ctrl_1h_rep2_shoot.35100000055cf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13615796
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC1301.495755E-820.09394320-21
CTCCAGT1551.1207521E-716.85276824-25
GCTTGAA1803.543937E-815.83461658-59
AGTCACT1652.2828499E-715.83153328-29
CCAGTCA1803.55285E-815.83141826-27
TGAACTC1703.2035496E-715.36595620-21
AGAGCAC3403.2046773E-715.36533458
CTCGTAT2101.4715624E-814.702950542-43
GTCACTG2001.2928649E-714.24838128-29
AACTCCA1858.3406485E-714.11975722-23
CAGTCAC1901.1264856E-613.74833626-27
ATCTCGT2153.117766E-713.25596240-41
CTGCTTG2401.1810571E-611.87598356-57
ACTCCAG2309.556319E-611.35719622-23
GACCAAT3001.2792589E-711.08278534-35
GAGCACA4306.0125487E-511.044899
TCCAGTC2653.8955823E-610.75339524-25
TGCTTGA2704.859645E-610.55641158-59
CCAATCT3701.9912477E-810.27000836-37
TCTCGTA2603.7001002E-510.04818940-41
Produced by FastQC (version 0.11.2)