FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l01n01_ctrl_1h_rep1_root.35100000055cc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l01n01_ctrl_1h_rep1_root.35100000055cc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40387281
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG7500.025.96957258-59
GCTTGAA8600.022.09255460-61
CTCGTAT9100.020.0964144-45
GCCGTCT11450.018.87578850-51
AATCTCG10350.018.12764440-41
CGTCTTC11950.017.29144352-53
ATCTCGT10850.017.07387742-43
TCTCGTA11750.016.1703542-43
GTCTTCT12150.016.03061954-55
TGCTTGA13300.015.53538460-61
CTTGAAA12700.015.3324262-63
TCTGCTT14300.013.78650758-59
GTATGCC15450.013.681540546-47
CAGTCAC15150.013.16693626-27
CCAGTCA15400.012.0279626-27
ACATGTC17350.011.90903632-33
TCACATG17600.011.60468730-31
GAATCTC16500.011.51491440-41
CCGTCTT18200.011.35344852-53
CATGTCA19650.010.87769132-33
Produced by FastQC (version 0.11.2)