FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_envcontrol.34200000035d42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_envcontrol.34200000035d42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14244084
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG492760.34594011099625643Illumina Single End PCR Primer 1 (100% over 50bp)
CGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT355010.24923329573175784No Hit
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG308260.21641265243872473No Hit
CTTTGTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAA272490.19130047253301793No Hit
GTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGAC206350.1448671602891418No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA186360.1308332638307946No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG180770.12690882755254743No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC171690.12053425127231769No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT161800.11359101785695731No Hit
TGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGACG152310.10692860277993306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTA34700.060.3130994-95
CGTGTAA29700.041.6845994-95
CTTTGTG1323300.041.2916341
GTATCAG84200.037.6877753
TTTGTGT1471550.037.1445662
ATCAGCT89550.035.9422655
TTGTGTT1562150.035.6028143
ATCGGAG51100.034.89933894-95
TCGGAAA69750.034.44261694-95
GCAAGAG111300.034.2870757
TGTGTTT1661150.033.6239934
CGGTATC95250.033.5113331
CAAGAGC116800.032.5564848
CGGGCAA118700.032.4206774
GTTTAGA48950.032.21690494-95
GGGCAAG119600.031.8649185
GGTATCA100350.031.4828052
CCGGGCA123450.031.3755023
AAGAGCA124250.030.5293039
GCTCACT106950.030.2762039
Produced by FastQC (version 0.11.2)