FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_8561.34200000035d0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_8561.34200000035d0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16320524
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCCG20900.093.7746794-95
GTTCTCG16300.076.8442894-95
CTTTGTG1973750.071.472481
CTAGTTA23300.067.67184494-95
TTTGTGT2145500.065.781682
TTGTGTT2432850.059.4412773
TGTGTTT2543950.057.1192674
TAACTTT182750.055.693631
CACTGTA12600.054.96769394-95
AACTTTG186900.054.6818772
ACTTTGT442250.052.8027731
GTGTTTG2853450.050.8960235
TCCTAGG11450.045.044694-95
TCTCGCG14800.042.8141194-95
ACATCCA64050.042.7931394-95
AGATCGA11250.040.60592394-95
TTTGAGG831000.037.463768
GTTTGAG2191750.035.8186537
TTGAGGG416650.035.518379
TTGAGGC144100.035.4196789