FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_7153.34200000035cc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_7153.34200000035cc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15753459
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA11550.0117.4253294-95
CTTTGTG1397200.066.345051
TTTGTGT1490150.062.2411352
ACTTTGT379750.054.6569671
TTGTGTT1759400.054.212613
TGTGTTT1834900.052.244634
AACTTTG174850.047.4776042
TAACTTT179750.046.2093281
GTGTTTG2146950.044.6467485
GCGTCGG9100.038.8799694-95
GTTCTCG13200.037.9717894-95
TTCGACA6600.035.73814494-95
TTGAGTC189850.035.6760754
TTTGAGG573700.033.4745838
GTTTGAA210150.033.018712
CGTAGTA13850.032.996594-95
TTTGAGT614050.032.2899253
ACATCCG18850.032.06479394-95
GTTTGAG1569350.031.6967837
TGTTTGA3055800.031.3274541
Produced by FastQC (version 0.11.2)