FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_7098.34200000035c86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_7098.34200000035c86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20734883
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA19500.077.9488394-95
CTTTGTG2944950.065.711691
TTTGTGT3168450.061.125192
TAACTTT409950.060.776611
AACTTTG434900.057.826552
TTGTGTT3542050.056.061763
TGTGTTT3792850.052.6270334
GTGTTTG4144350.048.152955
ACTTTGT843850.048.15161
GTTCTCG22250.047.75396794-95
GACCTAG13350.045.32205294-95
TTTGAGG1060650.039.2073948
GTTTGAG2758000.038.248657
TGTTTGA5308950.037.546416
TTGAGGG540500.037.3687069
GTTCCTA16950.036.5667794-95
GTTTGAC1103650.035.104317
TTTGAGA353700.034.2968648
TTTGAGC681850.033.02978
CAGTCTA14900.032.68395694-95
Produced by FastQC (version 0.11.2)