FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_7047.34200000035c43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_7047.34200000035c43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19069847
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG18550.076.27798594-95
CTTTGTG1683950.067.693541
TTTGTGT1817550.062.767462
TCTCGCG11950.059.2032194-95
TTGTGTT2042850.057.1570323
TAACTTT195900.056.647881
AACTTTG198250.056.3068352
TGTGTTT2188100.053.5826574
ACTTTGT440900.051.937641
CAGTCTA10300.050.08442794-95
GTGTTTG2456750.047.7162065
CTCGCTG18550.041.3172494-95
CGCCCGA8850.036.63965294-95
TTGAGTC224250.036.0255554
AGATCGA19700.035.1644394-95
TTTGAGG755200.033.9417088
GTTTGAG1954350.033.1757667
TTGAGGG392550.032.5777479
TTTGAGT720100.032.3843653
TGTTTGA3614350.032.383116
Produced by FastQC (version 0.11.2)