FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_619.34200000035c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_619.34200000035c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17202182
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412700.23991142519012998No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT384390.2234542106344416No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232400.13509914032998838No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT187120.10877689818652077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA12450.087.6417194-95
GTTCTCG10800.076.456294-95
ACATCCG15650.060.29955394-95
CTTTGTG1903150.060.0657231
TTTGTGT2081150.055.004562
ACTTTGT620750.053.3083531
TTGTGTT2252850.052.037543
TGTGTTT2401250.049.0468374
TAACTTT316600.048.376251
AACTTTG320800.048.077472
GTGTTTG2845000.041.3823435
TCTCGCG10350.039.8901994-95
CTGCGTA8950.037.892594-95
CCGCGGA7350.036.11050894-95
AGCACTA10250.034.5251794-95
GACCTAG8600.034.2909994-95
TTTGAGG929250.033.7436458
TTGAGGG538800.033.3194779
TTCGACA9850.032.93327394-95
GCTCTAG6750.032.76694594-95
Produced by FastQC (version 0.11.2)