FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_30515.34200000035be4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_30515.34200000035be4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18290016
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1850650.070.596991
CTAGTTA17700.064.9584194-95
TTTGTGT2030500.064.399432
TTGTGTT2264000.058.9520653
TAACTTT189950.057.36291
GTTCTCG21900.057.2122394-95
TGTGTTT2402750.055.7692874
AACTTTG200450.054.730882
AGATCGA18850.053.95749794-95
ACTTTGT444500.052.2806631
GTGTTTG2657600.050.382695
ACATCCG30200.043.44073594-95
TCTCGCG15000.038.32546694-95
TTTGAGG771250.036.592918
GTTTGAG2118000.035.8420757
TGTTTGA3789750.035.2928476
TTTGAGC574100.033.9457558
TTGAGGG393350.033.5672539
TTGAGGC151700.031.462899
GTTTGAC686250.031.433087
Produced by FastQC (version 0.11.2)