FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_30455.34200000035ba1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_30455.34200000035ba1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14181806
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1562350.073.49741
TTTGTGT1706050.067.369652
CTAGTTA14350.066.8263194-95
GTTCTCG16150.065.7727594-95
TTGTGTT1889650.062.0696833
TAACTTT151750.061.5025181
TGTGTTT2002850.058.783644
AACTTTG162500.057.9507982
GTGTTTG2196900.053.5687455
ACATCCG22650.052.75975894-95
ACTTTGT329500.047.4722981
AGATCGA13300.044.37050294-95
TTTGAGG660200.038.6576968
GTTTGAG1807500.036.9019247
TGTTTGA3206200.036.6443636
TTGAGGG333850.036.4904869
TTTGAGC483850.035.3802648
TTGAGTC241000.034.1025774
TTGAGGC129850.033.3090259
GTTTGAC573250.033.015137
Produced by FastQC (version 0.11.2)