FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n02_30429.34200000035b6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n02_30429.34200000035b6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14865804
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG14450.088.8389194-95
CTAGTTA10700.088.2567794-95
CTTTGTG1763900.072.3126451
TTTGTGT1913650.066.7295152
ACATCCG20600.065.8980394-95
TAACTTT179800.065.173921
AACTTTG184700.064.026022
TTGTGTT2148550.060.8444983
TGTGTTT2276200.057.7276544
GTGTTTG2572550.051.086675
AGATCGA12500.048.39780494-95
ACTTTGT387050.047.553951
TATATCG10300.041.5444194-95
TTTGAGG733500.038.758018
TTGAGGG341000.038.284579
GTTTAGA21400.037.9228394-95
GTTTGAG1940400.037.0111477
GCTCTAG8500.036.4545994-95
TGTTTGA3634700.036.0990146
TTGAGTC224000.035.400424
Produced by FastQC (version 0.11.2)