FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_envcontrol.34100000035d45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_envcontrol.34100000035d45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14244084
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCAGAACTCCAGTCACTGGTGAATCTCGTATGC1842871.2937792279236768TruSeq Adapter, Index 4 (97% over 35bp)
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG337100.23665965463275843No Hit
CGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT307480.21586505667896932No Hit
CTTTGTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAA252370.1771753101147115No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG230230.1616320150878077No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT209410.14701542057741307No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA171880.12066763998302733No Hit
GTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGAC167890.11786647705812463No Hit
TGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGACG167320.11746631092599566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1259300.040.3167881
GTATCAG84050.038.5558933
TTTGTGT1392000.036.487322
TTGTGTT1474000.035.2891963
CTCGTAT299850.034.70071442-43
ATCAGCT94350.034.57585
CGTATGC307250.034.5682344-45
TCGTATG303150.034.3934742-43
TATGCCG313500.033.65965746-47
TGTGTTT1558250.033.5275844
GTATGCC307900.033.43872544-45
CGGTATC99300.033.2105031
CCGTCTT322350.032.6288350-51
GCAAGAG96000.032.497627
GCCGTCT316400.032.2030348-49
TCTTCTG318800.031.73443652-53
GCTTGAA294300.031.62206358-59
ATGCCGT323900.031.3179646-47
TCTGCTT340000.031.02586656-57
TAATACG54000.030.73330724-25
Produced by FastQC (version 0.11.2)