FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_8561.34100000035d01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_8561.34100000035d01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16320524
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1829850.072.462721
TTTGTGT1987800.066.872932
TTGTGTT2272500.060.0821083
TGTGTTT2389350.057.4480934
TAACTTT173100.057.3777161
AACTTTG179250.055.8015022
ACTTTGT415500.053.2712861
GTGTTTG2665850.051.473885
TTTGAGG796150.037.5631988
GTTTGAG2066300.036.3782967
TGTTTGA3842800.035.684056
TTGAGGG407750.035.2278569
TTGAGGC138850.034.916829
TTTGAGC515400.034.300748
TTGAGGT233500.031.5006399
GTTTGAC735950.031.4672837
GTTTGAA291550.030.151692
TTTGAGA294000.029.1530978
TTTGACC194000.028.9559488
TTTGAGT804400.028.9393233