##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l08n01_7153.34100000035ccc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15753459 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73932550305301 33.0 33.0 33.0 33.0 33.0 2 32.7676211300642 33.0 33.0 33.0 33.0 33.0 3 32.782411278691235 33.0 33.0 33.0 33.0 33.0 4 36.696652335210956 37.0 37.0 37.0 37.0 37.0 5 36.712516914539215 37.0 37.0 37.0 37.0 37.0 6 36.663438677181944 37.0 37.0 37.0 37.0 37.0 7 36.60672471994881 37.0 37.0 37.0 37.0 37.0 8 36.622219729647945 37.0 37.0 37.0 37.0 37.0 9 36.606853517059335 37.0 37.0 37.0 37.0 37.0 10-11 36.58427377758751 37.0 37.0 37.0 37.0 37.0 12-13 36.54684739395964 37.0 37.0 37.0 37.0 37.0 14-15 38.60857853503792 40.0 37.0 40.0 37.0 40.0 16-17 38.59008859578078 40.0 37.0 40.0 37.0 40.0 18-19 38.535648742285744 40.0 37.0 40.0 37.0 40.0 20-21 38.475294949509184 40.0 37.0 40.0 37.0 40.0 22-23 38.42266803119239 40.0 37.0 40.0 37.0 40.0 24-25 38.38189222443147 40.0 37.0 40.0 37.0 40.0 26-27 38.26000464405944 40.0 37.0 40.0 37.0 40.0 28-29 38.19550655510006 40.0 37.0 40.0 37.0 40.0 30-31 38.12032262247929 40.0 37.0 40.0 37.0 40.0 32-33 38.022885608805026 40.0 37.0 40.0 37.0 40.0 34-35 37.93605674157021 40.0 37.0 40.0 35.0 40.0 36-37 37.81810232914562 40.0 37.0 40.0 33.0 40.0 38-39 37.69817435015383 40.0 37.0 40.0 33.0 40.0 40-41 37.551815064869245 40.0 37.0 40.0 33.0 40.0 42-43 37.39622441014383 40.0 37.0 40.0 33.0 40.0 44-45 37.1881218277205 37.0 37.0 40.0 33.0 40.0 46-47 36.971698564740606 37.0 37.0 40.0 33.0 40.0 48-49 36.73943976367349 37.0 37.0 40.0 33.0 40.0 50-51 36.50159069827141 37.0 37.0 40.0 33.0 40.0 52-53 36.238254500170406 37.0 37.0 40.0 33.0 40.0 54-55 35.969508410819486 37.0 37.0 40.0 33.0 40.0 56-57 35.66296046474619 37.0 33.0 38.5 33.0 40.0 58-59 35.39068911786294 37.0 33.0 37.0 33.0 40.0 60-61 35.094800894203615 37.0 33.0 37.0 30.0 40.0 62-63 34.80283920502792 37.0 33.0 37.0 27.0 40.0 64-65 34.55121570443672 37.0 33.0 37.0 27.0 38.5 66-67 34.30251356225956 37.0 33.0 37.0 27.0 37.0 68-69 34.066243673849655 37.0 33.0 37.0 27.0 37.0 70-71 33.84759708962965 37.0 33.0 37.0 27.0 37.0 72-73 33.6587958238251 37.0 33.0 37.0 27.0 37.0 74-75 33.422024267813185 37.0 33.0 37.0 27.0 37.0 76-77 30.553750608041064 33.0 30.0 33.0 24.5 35.0 78-79 33.19692675113447 35.0 33.0 37.0 27.0 37.0 80-81 33.73193185699725 37.0 33.0 37.0 27.0 37.0 82-83 33.71564721119343 37.0 33.0 37.0 27.0 37.0 84-85 33.596550605171856 37.0 33.0 37.0 27.0 37.0 86-87 33.51377427649382 37.0 33.0 37.0 27.0 37.0 88-89 33.36434261834179 37.0 33.0 37.0 27.0 37.0 90-91 33.260326763792 37.0 33.0 37.0 27.0 37.0 92-93 33.15384091201811 35.0 33.0 37.0 27.0 37.0 94-95 33.0282495101552 33.0 33.0 37.0 27.0 37.0 96-97 32.90102732358652 33.0 33.0 37.0 27.0 37.0 98-99 32.738135827820415 33.0 33.0 37.0 27.0 37.0 100-101 30.59644002628248 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09961224091723864 2306 2 0.09147573196853642 2306 3 0.09158848288411292 2306 4 0.07963863717133535 2306 5 0.08040594253675692 2306 6 0.11164221369134708 2306 7 0.14040732585298343 2306 8 0.06863585448503784 2306 9 0.12727923180268164 2306 10-11 0.09592919621905338 2306 12-13 0.0943306308181846 2306 14-15 0.1737080015675545 2306 16-17 0.16681152205756433 2306 18-19 0.11193537048912816 2306 20-21 0.12567005162328115 2306 22-23 0.13558868339124075 2306 24-25 0.15816701024076707 2306 26-27 0.18565890897370707 2306 28-29 0.1254794696938859 2306 30-31 0.0863876058050721 2306 32-33 0.08681153719935963 2306 34-35 0.13264165395168703 2306 36-37 0.04934684787949806 2306 38-39 0.05808847835036346 2306 40-41 0.10491246128922427 2306 42-43 0.039250466660980976 2306 44-45 0.036014369787324085 2306 46-47 -0.0031970616607495117 2306 48-49 -0.03488701557500207 2306 50-51 -0.18272859764939398 2306 52-53 -0.08655918464119594 2306 54-55 -0.11831481579557135 2306 56-57 -0.2775035448279297 2306 58-59 -0.22650727246654156 2306 60-61 -0.2316939637205948 2306 62-63 -0.2425911593108836 2306 64-65 -0.4395724875250977 2306 66-67 -0.4756267360994144 2306 68-69 -0.45916680463260917 2306 70-71 -0.5470737149143048 2306 72-73 -0.5319105759070837 2306 74-75 -0.5946842456291179 2306 76-77 -0.8825675360086827 2306 78-79 -0.5383169466248887 2306 80-81 -0.5797001691105734 2306 82-83 -0.4741727727343914 2306 84-85 -0.5205684136451225 2306 86-87 -0.4586477528658719 2306 88-89 -0.6430711712443227 2306 90-91 -0.7101847285713561 2306 92-93 -0.6063435397273835 2306 94-95 -0.8523829087237047 2306 96-97 -0.8201326799447415 2306 98-99 -0.6496000151695114 2306 100-101 -0.9438590472796555 2307 1 0.08979233449417734 2307 2 0.07395409817634402 2307 3 0.06852389049326746 2307 4 0.07904348632754932 2307 5 0.06658465540333935 2307 6 0.07240587481219762 2307 7 0.10194413349766762 2307 8 0.0643219469187386 2307 9 0.04955325632980845 2307 10-11 0.06701885965173915 2307 12-13 0.1046959187210561 2307 14-15 0.17338514641836156 2307 16-17 0.13539581442871196 2307 18-19 0.14925276642298968 2307 20-21 0.15129650814267137 2307 22-23 0.18087074187726415 2307 24-25 0.07277711117323094 2307 26-27 0.1685130124614176 2307 28-29 0.11996686136404833 2307 30-31 0.11456823814132377 2307 32-33 0.03612028658437083 2307 34-35 0.11544389703232838 2307 36-37 0.05657630095058863 2307 38-39 -0.029203816181869513 2307 40-41 0.00414577066261046 2307 42-43 -0.017858223753442815 2307 44-45 -0.043426146789045106 2307 46-47 -0.026043186058032575 2307 48-49 -0.01624808256620014 2307 50-51 -0.07830706831543921 2307 52-53 -0.1275079481963104 2307 54-55 -0.07137077151211457 2307 56-57 -0.1852543612580959 2307 58-59 -0.17692575015950496 2307 60-61 -0.19758837189568368 2307 62-63 -0.26675104895661406 2307 64-65 -0.44310788927806044 2307 66-67 -0.39272780559782916 2307 68-69 -0.4087014628246166 2307 70-71 -0.48737468855092914 2307 72-73 -0.43591730829161435 2307 74-75 -0.531654485891174 2307 76-77 -0.9528373753781132 2307 78-79 -0.5354754919488514 2307 80-81 -0.3666079046360622 2307 82-83 -0.27439526162295635 2307 84-85 -0.2632252668487851 2307 86-87 -0.26622505270008645 2307 88-89 -0.3334536225811817 2307 90-91 -0.4182770139245875 2307 92-93 -0.4352637324374129 2307 94-95 -0.526082580410268 2307 96-97 -0.6150355679056148 2307 98-99 -0.5856914398379729 2307 100-101 -0.7382545098418056 2201 1 0.06808440269069393 2201 2 0.05952014988158538 2201 3 0.058968294654917486 2201 4 0.061459497936894536 2201 5 0.07960439468585179 2201 6 0.09325829422296295 2201 7 0.11966105023017803 2201 8 0.09298697823567892 2201 9 0.0850836502009571 2201 10-11 0.0923131675069655 2201 12-13 0.08231299705489192 2201 14-15 0.19710769660752447 2201 16-17 0.1597410923422231 2201 18-19 0.14498062855041383 2201 20-21 0.16785822459362265 2201 22-23 0.2103344408691541 2201 24-25 0.1493582576389869 2201 26-27 0.14294034720681736 2201 28-29 0.15327213394456152 2201 30-31 0.16205309689339487 2201 32-33 0.16959358341842545 2201 34-35 0.17363651819403714 2201 36-37 0.15851022508237378 2201 38-39 0.18034722024911076 2201 40-41 0.1060917046283052 2201 42-43 0.10847150862373667 2201 44-45 0.07331002416162136 2201 46-47 0.10623938102137487 2201 48-49 0.07900930994391331 2201 50-51 0.10131205329390269 2201 52-53 -0.032190869342322515 2201 54-55 -0.05972357117330773 2201 56-57 0.012747684724885744 2201 58-59 0.0438994896464493 2201 60-61 -0.008804429868668251 2201 62-63 -0.03319042151313312 2201 64-65 -0.06695271719933515 2201 66-67 -0.053582545019466465 2201 68-69 -0.11167410130085642 2201 70-71 -0.19414807815675061 2201 72-73 -0.27708606886169207 2201 74-75 -0.32140073240037026 2201 76-77 -0.8751099132857867 2201 78-79 -0.12438936580470994 2201 80-81 -0.04696828514588702 2201 82-83 0.15534084076890764 2201 84-85 0.09417170937200581 2201 86-87 -0.008637104570546228 2201 88-89 -0.055177285773091 2201 90-91 -0.07721146353157593 2201 92-93 0.05776991751954341 2201 94-95 -0.05176664838413103 2201 96-97 -0.12621540917103147 2201 98-99 -0.02974522472203489 2201 100-101 -0.25532839890732006 1101 1 0.06260087698722572 1101 2 0.07936573236347044 1101 3 0.06784953962247187 1101 4 0.031666613478769534 1101 5 0.05138042011174804 1101 6 0.07439184991151393 1101 7 0.0711473575078756 1101 8 0.055045405555254945 1101 9 0.05539203107440471 1101 10-11 0.060458401715294485 1101 12-13 0.08308076012902887 1101 14-15 0.1433202335126751 1101 16-17 0.12416178034210645 1101 18-19 0.1415620523074992 1101 20-21 0.12429980266469443 1101 22-23 0.1494705313791016 1101 24-25 0.1033523902260356 1101 26-27 0.04861130233229005 1101 28-29 0.08829260632196423 1101 30-31 0.11888020769092833 1101 32-33 0.06440895956145454 1101 34-35 0.10676135944203224 1101 36-37 0.0538856658135316 1101 38-39 0.03999890704563569 1101 40-41 0.05073170074680178 1101 42-43 0.07163168492482441 1101 44-45 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4 0.02029083363200357 2304 5 0.05162649990666068 2304 6 0.03691196651493556 2304 7 0.06577978696149955 2304 8 0.047755094760745465 2304 9 0.04640828805918318 2304 10-11 0.024860794591752722 2304 12-13 0.028034465930836916 2304 14-15 0.03680128060708654 2304 16-17 0.058799959967004156 2304 18-19 0.06436634189562795 2304 20-21 0.06385106974930466 2304 22-23 0.06752200662450747 2304 24-25 0.05159745891700851 2304 26-27 0.02567103939416171 2304 28-29 -0.009494349571468774 2304 30-31 0.03679677346710264 2304 32-33 0.007611992184891392 2304 34-35 0.032387950015497324 2304 36-37 0.010749649856158783 2304 38-39 0.01065071811229501 2304 40-41 0.037454108855534685 2304 42-43 -0.019320230741136868 2304 44-45 -0.03024434482458105 2304 46-47 -0.051357024703229115 2304 48-49 -0.044889265692766855 2304 50-51 -0.1093260635274973 2304 52-53 -0.05871003763568439 2304 54-55 -0.07080523326879984 2304 56-57 -0.06938832111006121 2304 58-59 -0.011814623735652674 2304 60-61 -0.09031730744613498 2304 62-63 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18-19 0.0378617816884983 2202 20-21 0.056626608597227346 2202 22-23 0.07013285965841476 2202 24-25 0.03327718886104947 2202 26-27 0.024149795981088573 2202 28-29 0.05424814691131985 2202 30-31 -0.017643496141360515 2202 32-33 -0.06756760252704197 2202 34-35 -0.02701643696108391 2202 36-37 -0.02445190976844458 2202 38-39 -0.04457491832257432 2202 40-41 0.01235547185383723 2202 42-43 -0.04060635282608871 2202 44-45 -0.02874135480153228 2202 46-47 -0.05984429659473989 2202 48-49 -0.0051789568061550995 2202 50-51 -0.03306235483903919 2202 52-53 -0.06038055648730989 2202 54-55 -0.033471834707398784 2202 56-57 -0.03971597829333717 2202 58-59 -0.016553144904875694 2202 60-61 -0.14664255851856467 2202 62-63 -0.09419775537491404 2202 64-65 -0.145189642431383 2202 66-67 -0.1426288468921797 2202 68-69 -0.18065407256266752 2202 70-71 -0.21417849087720953 2202 72-73 -0.13786506514375674 2202 74-75 -0.13682566574571098 2202 76-77 -0.5298257600085385 2202 78-79 -0.09480495012339674 2202 80-81 0.03290232486703104 2202 82-83 0.07494673482726455 2202 84-85 0.02736079110312062 2202 86-87 0.010982008927207687 2202 88-89 9.764990065406209E-4 2202 90-91 -0.024145096000218302 2202 92-93 0.02879002334558578 2202 94-95 0.06773972868504785 2202 96-97 -0.07036031566529033 2202 98-99 0.002740076009644099 2202 100-101 -0.02563604839283684 1102 1 0.05025184708789254 1102 2 0.03439606828629138 1102 3 0.04017659697017706 1102 4 -0.0034127801457586315 1102 5 0.025033046791975266 1102 6 0.041812424156233874 1102 7 -0.004084973224877331 1102 8 0.01793840030506999 1102 9 0.03578630490162027 1102 10-11 0.043774625667069245 1102 12-13 0.023756780028449498 1102 14-15 -0.006160692227233255 1102 16-17 -0.023720884614931492 1102 18-19 0.010268553470119457 1102 20-21 -0.0014060313502213262 1102 22-23 -0.010517870688836695 1102 24-25 -0.03833366378511727 1102 26-27 -0.009919905164039733 1102 28-29 -0.042766369762162526 1102 30-31 -0.0525743244230128 1102 32-33 -0.03196918339746446 1102 34-35 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0.33706626698043607 1213 66-67 0.29827832821187883 1213 68-69 0.3620847344675937 1213 70-71 0.33359956472315133 1213 72-73 0.43282984693773585 1213 74-75 0.4634932216172629 1213 76-77 0.8838922569093164 1213 78-79 0.3277200050354807 1213 80-81 0.2324752022615897 1213 82-83 0.1382678026501054 1213 84-85 0.21856345554668621 1213 86-87 0.32901887700771226 1213 88-89 0.37710439260532524 1213 90-91 0.3387696447062609 1213 92-93 0.4400304815456977 1213 94-95 0.42190861126622536 1213 96-97 0.5391220698608308 1213 98-99 0.6184650486026442 1213 100-101 0.8099334885169647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 49.0 8 363.0 9 1189.0 10 775.0 11 285.0 12 316.0 13 648.0 14 1689.0 15 3735.0 16 6649.0 17 9967.0 18 13445.0 19 17765.0 20 22626.0 21 29214.0 22 36831.0 23 46908.0 24 59766.0 25 75688.0 26 95919.0 27 121798.0 28 156599.0 29 199799.0 30 260324.0 31 345067.0 32 475649.0 33 694348.0 34 1114969.0 35 2079730.0 36 4326036.0 37 4933153.0 38 622128.0 39 30.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.08607188440677 21.915100953859294 30.460144478823175 26.538682682910764 2 28.022690127926825 21.99600100523955 29.466328632968796 20.51498023386483 3 22.225036418985823 20.250650984015635 36.359456040733654 21.16485655626488 4 22.02833397152018 20.471079718134 35.47568254019878 22.024903770147034 5 28.677194005456197 19.656838539396333 31.05913437804358 20.606833077103893 6 30.07072922841898 20.230084072329767 29.69526248171909 20.003924217532163 7 28.686956940694742 26.01683223982746 24.7417598890504 20.554450930427407 8 26.957425667594652 21.71719874346326 29.747466889652614 21.57790869928947 9 23.44975792300599 20.917691790736246 33.25704532572814 22.37550496052962 10-11 27.896477533040038 21.586175793794265 27.535406236789324 22.981940436376373 12-13 26.94652012615134 25.155913377500138 24.324527711660025 23.573038784688492 14-15 26.6675020387586 23.208417275215556 25.717862978536964 24.406217707488874 16-17 26.255779762400117 22.887424279328116 25.311501429622535 25.545294528649233 18-19 26.18796608414698 23.54608279997428 25.735097288792257 24.53085382708648 20-21 25.259133876189665 24.101296969934346 25.96135970945558 24.67820944442041 22-23 25.4718281235886 24.03969185434132 25.60827752178109 24.880202500288984 24-25 25.4342427272639 24.090569569514862 25.366743265717073 25.10844443750417 26-27 25.457048018936735 24.093504752703964 25.381428920697896 25.068018307661404 28-29 25.36064303002894 24.434078468811197 25.094817107048534 25.110461394111333 30-31 25.563304926215647 24.112632133660846 25.040932587246438 25.28313035287707 32-33 25.403468533482076 24.340635285241166 25.05911876242545 25.196777418851312 34-35 25.348382218787634 24.560463197320665 24.784680621570157 25.306473962321547 36-37 25.53149695081145 24.311066141782817 25.011115816604914 25.14632109080082 38-39 25.516122932180412 24.19343443297748 24.884346960958002 25.406095673884106 40-41 25.46630959125928 24.51690508846421 24.832250743389572 25.18453457688694 42-43 25.51291116446236 24.41311777940324 24.847374789244697 25.226596266889707 44-45 25.643628510747497 24.225165834064526 25.070438315472813 25.060767339715163 46-47 25.360598020695274 24.47013245284104 25.001156988812344 25.16811253765134 48-49 25.457363285049517 24.235435163290084 25.016350131402525 25.290851420257876 50-51 25.30816401715053 24.582344810748793 25.279947342416143 24.829543829684532 52-53 25.354850640056004 24.471188528888156 25.25547075025437 24.91849008080147 54-55 25.298805849448165 24.405166432529146 24.95864067751301 25.33738704050968 56-57 25.38545333593975 24.51022226138761 25.143266698491367 24.961057704181275 58-59 25.226859798931596 24.514058023550835 25.16846095393098 25.090621223586595 60-61 25.64610862644147 24.562716835479602 24.746263208514392 25.044911329564535 62-63 25.21907541628257 24.843114911608225 25.104531074897775 24.833278597211432 64-65 25.440906631546184 24.752759143300228 24.839135085135585 24.967199140018 66-67 25.626714947028024 24.86844988932999 24.73560841424164 24.769226749400346 68-69 25.447773558303886 24.766480358039168 24.95567891052008 24.830067173136865 70-71 25.34493138893575 24.83891689375762 24.763915454169315 25.052236263137313 72-73 25.461738237584257 24.588305409136137 24.860265255613715 25.089691097665888 74-75 25.203585712879423 24.58055846557184 25.013009759486188 25.202846062062545 76-77 25.346946973312544 24.459439363002094 24.847288976917742 25.346324686767623 78-79 25.418899778912685 24.450176384868154 24.884847710780107 25.24607612543906 80-81 25.26034524550215 24.46739863089729 25.015859217943692 25.256396905656864 82-83 25.268962250479 24.919805350431876 24.830471307064396 24.98076109202473 84-85 25.229582848469036 24.86964054335274 24.746480250231244 25.154296357946983 86-87 25.075851347294257 24.9815589377872 24.831852874256747 25.110736840661797 88-89 25.05951110834872 25.453482740207278 24.48432223934325 25.002683912100753 90-91 25.074571018453895 24.964022663761753 24.74031040195359 25.22109591583076 92-93 24.959739846136497 25.291357546229655 24.528913025369295 25.219989582264557 94-95 25.11221013723823 25.34480035543303 24.288818859792052 25.25417064753669 96-97 25.0562962180395 25.024770462916557 24.405902525450752 25.513030793593188 98-99 25.09470336395631 25.184226602236603 24.524281606964983 25.196788426842105 100-101 25.53997910689059 25.4588034978283 23.85979145234754 25.14142594293357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37333.0 1 21196.0 2 8382.5 3 10423.5 4 9538.5 5 7280.5 6 4359.0 7 4254.0 8 4504.5 9 4528.0 10 4064.5 11 3319.0 12 2763.0 13 2538.5 14 2523.0 15 2631.5 16 2832.0 17 3011.0 18 3233.0 19 3473.5 20 3810.0 21 4228.0 22 4756.0 23 5409.0 24 6150.5 25 7135.0 26 8163.0 27 9305.5 28 10604.0 29 12047.0 30 14091.0 31 17155.5 32 24815.0 33 38607.5 34 58085.0 35 78656.5 36 99941.0 37 134778.0 38 174953.0 39 200242.0 40 215349.5 41 236729.5 42 276343.0 43 328027.0 44 397411.0 45 490195.0 46 596228.0 47 711861.5 48 826456.0 49 915708.5 50 927270.5 51 901096.0 52 902579.5 53 933690.0 54 939961.5 55 921907.5 56 895124.0 57 791164.0 58 652076.0 59 531338.5 60 428113.0 61 322853.5 62 216692.5 63 135080.5 64 80189.0 65 48476.5 66 28562.0 67 16907.0 68 11305.0 69 8063.5 70 6062.5 71 4743.5 72 4123.0 73 3512.5 74 2620.0 75 1940.0 76 1436.5 77 1165.0 78 1073.5 79 922.0 80 662.5 81 452.0 82 306.5 83 233.0 84 186.0 85 135.0 86 109.0 87 107.0 88 85.0 89 61.5 90 59.0 91 54.5 92 43.0 93 31.0 94 29.5 95 35.5 96 26.0 97 13.5 98 10.5 99 5.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15295053613304863 2 0.0 3 0.0 4 0.06946410943780665 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002142386633944964 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0034881228306748377 22-23 0.0 24-25 0.0 26-27 0.0010378673026666715 28-29 6.633463799918482E-4 30-31 0.0026882984873353847 32-33 0.0 34-35 0.0 36-37 3.4912967367992006E-5 38-39 5.713031023853237E-4 40-41 7.01433253484203E-4 42-43 0.0 44-45 8.664763719510744E-4 46-47 0.008645720282764566 48-49 0.0033484709612028696 50-51 0.0023010819401631097 52-53 0.00491955449276251 54-55 0.006122464913896053 56-57 0.016440833724199872 58-59 0.019979739052864515 60-61 0.008309286233582097 62-63 0.003856295941100935 64-65 0.012482972787119325 66-67 0.029279283997247846 68-69 0.021192171192371146 70-71 0.01541248813990629 72-73 0.023534513912150976 74-75 0.01766913539432832 76-77 0.03229132090926824 78-79 0.0025835595852314085 80-81 1.5869530621814547E-5 82-83 0.0014346055682120352 84-85 0.01034058615317436 86-87 0.012225886391045928 88-89 0.01395249132269935 90-91 0.009702631022177414 92-93 0.02155082258442416 94-95 0.02321077548746596 96-97 0.018059525847624956 98-99 0.021319127437345667 100-101 0.33989995467027273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5753459E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.847228053619176 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30111743625208 16.66967913375778 2 8.78105286411284 3.8368332894631805 3 3.0828462020940526 2.020549320941475 4 1.9706239377763164 1.722106823060809 5 1.3031459067454798 1.4235062905904423 6 0.8671680451470275 1.1367130825882943 7 0.8530494014672423 1.3045735370400728 8 0.7260665120460565 1.2690032536612823 9 0.43849566944499685 0.8621923401800329 >10 4.22092514799925 19.696051111041207 >50 0.7486131916590196 11.59608610750981 >100 0.642032326695352 27.276864828241948 >500 0.05497283630391261 8.081798291169374 >1k 0.009832653987857654 2.8404282954406375 >5k 0.0 0.0 >10k+ 5.786826857864563E-5 0.2636142953135745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24779 0.1572924397111771 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16924 0.10743037449743578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.252155923343565E-5 0.0 0.0 2 8.252155923343565E-5 0.0 0.0 3 8.252155923343565E-5 0.0 0.0 4 8.252155923343565E-5 0.0 0.0 5 8.252155923343565E-5 0.0 0.0 6 8.252155923343565E-5 0.0 0.0 7 9.52171837308873E-5 0.0 0.0 8 1.1426062047706475E-4 0.0 0.0 9 1.4599968172069385E-4 0.0 0.0 10-11 1.7773874296432294E-4 0.0 0.0 12-13 3.0469498793883935E-4 0.0 0.0 14-15 4.538685757838961E-4 0.0 6.347812248725819E-6 16-17 6.601724738674853E-4 6.347812248725819E-6 1.2695624497451638E-5 18-19 9.489979311845101E-4 6.347812248725819E-6 1.2695624497451638E-5 20-21 0.0013584318212273255 6.347812248725819E-6 1.2695624497451638E-5 22-23 0.0018027786786381328 6.347812248725819E-6 1.2695624497451638E-5 24-25 0.0022598211605463917 6.347812248725819E-6 1.2695624497451638E-5 26-27 0.0027739939526931832 6.347812248725819E-6 1.2695624497451638E-5 28-29 0.0034246447081875795 6.347812248725819E-6 1.2695624497451638E-5 30-31 0.004141947492293597 6.347812248725819E-6 1.2695624497451638E-5 32-33 0.0048275112151559855 1.2695624497451638E-5 1.2695624497451638E-5 34-35 0.005687639774858334 1.2695624497451638E-5 1.2695624497451638E-5 36-37 0.006700115828530103 1.2695624497451638E-5 1.2695624497451638E-5 38-39 0.007890330625166194 1.2695624497451638E-5 1.2695624497451638E-5 40-41 0.009407457752611665 1.2695624497451638E-5 1.2695624497451638E-5 42-43 0.010886498006564781 1.2695624497451638E-5 1.2695624497451638E-5 44-45 0.012606755125969479 1.2695624497451638E-5 1.2695624497451638E-5 46-47 0.01446349020872178 1.2695624497451638E-5 1.2695624497451638E-5 48-49 0.01683122417749651 1.2695624497451638E-5 1.2695624497451638E-5 50-51 0.019811522028273285 1.2695624497451638E-5 1.2695624497451638E-5 52-53 0.02356942687951897 1.2695624497451638E-5 1.2695624497451638E-5 54-55 0.027866895771906347 1.2695624497451638E-5 1.2695624497451638E-5 56-57 0.03313875384447314 1.2695624497451638E-5 1.2695624497451638E-5 58-59 0.03929613172573719 1.2695624497451638E-5 1.2695624497451638E-5 60-61 0.04727850562850991 1.2695624497451638E-5 1.2695624497451638E-5 62-63 0.05733978804274033 1.2695624497451638E-5 1.2695624497451638E-5 64-65 0.0700925428504305 1.2695624497451638E-5 1.2695624497451638E-5 66-67 0.08576211738641018 1.2695624497451638E-5 1.2695624497451638E-5 68-69 0.1053990745778435 1.2695624497451638E-5 1.2695624497451638E-5 70-71 0.12983497782931355 1.2695624497451638E-5 1.904343674617746E-5 72-73 0.1599426513250201 1.2695624497451638E-5 1.904343674617746E-5 74-75 0.19784226435603763 1.2695624497451638E-5 1.904343674617746E-5 76-77 0.24426064142484516 1.2695624497451638E-5 1.904343674617746E-5 78-79 0.3024415145905417 1.2695624497451638E-5 1.904343674617746E-5 80-81 0.375016052030224 1.2695624497451638E-5 1.904343674617746E-5 82-83 0.4640504666308523 1.2695624497451638E-5 1.904343674617746E-5 84-85 0.5722521003165082 1.2695624497451638E-5 1.904343674617746E-5 86-87 0.7041564649389065 1.2695624497451638E-5 1.904343674617746E-5 88-89 0.8620868597810805 1.2695624497451638E-5 1.904343674617746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 130955 0.0 66.269585 1 TTTGTGT 139280 0.0 62.344032 2 ACTTTGT 37335 0.0 54.26181 1 TTGTGTT 165690 0.0 54.009792 3 TGTGTTT 173385 0.0 51.886826 4 AACTTTG 18410 0.0 49.088863 2 TAACTTT 18760 0.0 47.918797 1 GTGTTTG 201775 0.0 44.683838 5 TTGAGTC 17865 0.0 36.06475 4 TTTGAGG 55210 0.0 33.999344 8 GTTTGAA 20055 0.0 32.576637 2 GTTTGAG 148595 0.0 32.39473 2 TGTTTGA 287725 0.0 32.2823 1 TTTGAGT 57730 0.0 32.032993 3 TTGAGGG 31035 0.0 30.295279 9 TTTGACC 15430 0.0 29.851582 8 GTTTGAC 57960 0.0 29.190996 7 TGACTCG 1370 0.0 28.42201 5 TTGATTC 10575 0.0 28.216652 4 TTGAGGC 11005 0.0 27.572256 9 >>END_MODULE