FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_7153.34100000035ccc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_7153.34100000035ccc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15753459
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247790.1572924397111771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169240.10743037449743578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1309550.066.2695851
TTTGTGT1392800.062.3440322
ACTTTGT373350.054.261811
TTGTGTT1656900.054.0097923
TGTGTTT1733850.051.8868264
AACTTTG184100.049.0888632
TAACTTT187600.047.9187971
GTGTTTG2017750.044.6838385
TTGAGTC178650.036.064754
TTTGAGG552100.033.9993448
GTTTGAA200550.032.5766372
GTTTGAG1485950.032.394732
TGTTTGA2877250.032.28231
TTTGAGT577300.032.0329933
TTGAGGG310350.030.2952799
TTTGACC154300.029.8515828
GTTTGAC579600.029.1909967
TGACTCG13700.028.422015
TTGATTC105750.028.2166524
TTGAGGC110050.027.5722569
Produced by FastQC (version 0.11.2)