FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_7098.34100000035c89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_7098.34100000035c89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20734883
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG2797050.065.677371
TAACTTT411300.061.9808961
TTTGTGT3004800.061.218282
AACTTTG437900.059.0908362
TTGTGTT3385600.055.9690363
TGTGTTT3643250.052.403474
ACTTTGT838800.048.5635341
GTGTTTG3954800.048.2533455
TTTGAGG1006700.038.6596078
GTTTGAG2647300.038.0015537
TGTTTGA5055750.037.7080356
TTGAGGG518550.036.9037069
GTTTGAC1036500.035.4132427
TTTGAGA341250.033.6325268
TTTGATG367600.033.1464278
TTTGACA126700.032.606058
TTTGAGC675950.032.3216258
GTTTGAA338750.031.595867
TTGACCC178750.030.8685079
TTGAGGT327500.030.346849