##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l08n01_7047.34100000035c46.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19069847 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.743573768578216 33.0 33.0 33.0 33.0 33.0 2 32.77278616865673 33.0 33.0 33.0 33.0 33.0 3 32.78840658763544 33.0 33.0 33.0 33.0 33.0 4 36.7048902385006 37.0 37.0 37.0 37.0 37.0 5 36.72191460162213 37.0 37.0 37.0 37.0 37.0 6 36.67028697188813 37.0 37.0 37.0 37.0 37.0 7 36.61607903828489 37.0 37.0 37.0 37.0 37.0 8 36.632301402313296 37.0 37.0 37.0 37.0 37.0 9 36.617478315374 37.0 37.0 37.0 37.0 37.0 10-11 36.597927739011226 37.0 37.0 37.0 37.0 37.0 12-13 36.56457335499336 37.0 37.0 37.0 37.0 37.0 14-15 38.650567647448874 40.0 37.0 40.0 37.0 40.0 16-17 38.636735365522334 40.0 37.0 40.0 37.0 40.0 18-19 38.59051582846994 40.0 37.0 40.0 37.0 40.0 20-21 38.53277197242327 40.0 37.0 40.0 37.0 40.0 22-23 38.477831940654795 40.0 37.0 40.0 37.0 40.0 24-25 38.43928739963147 40.0 37.0 40.0 37.0 40.0 26-27 38.32890811866503 40.0 37.0 40.0 37.0 40.0 28-29 38.27171581397585 40.0 37.0 40.0 37.0 40.0 30-31 38.20789883631473 40.0 37.0 40.0 37.0 40.0 32-33 38.12806896143425 40.0 37.0 40.0 37.0 40.0 34-35 38.05538230065506 40.0 37.0 40.0 37.0 40.0 36-37 37.953620708126294 40.0 37.0 40.0 35.0 40.0 38-39 37.846933119075366 40.0 37.0 40.0 33.0 40.0 40-41 37.7326025478862 40.0 37.0 40.0 33.0 40.0 42-43 37.59854992543988 40.0 37.0 40.0 33.0 40.0 44-45 37.41557056016234 40.0 37.0 40.0 33.0 40.0 46-47 37.22902535610275 38.5 37.0 40.0 33.0 40.0 48-49 37.02089363905227 37.0 37.0 40.0 33.0 40.0 50-51 36.81347957327607 37.0 37.0 40.0 33.0 40.0 52-53 36.56345965963963 37.0 37.0 40.0 33.0 40.0 54-55 36.31254388144802 37.0 37.0 40.0 33.0 40.0 56-57 36.026654985747925 37.0 37.0 40.0 33.0 40.0 58-59 35.76523041847163 37.0 35.0 38.5 33.0 40.0 60-61 35.47243027172688 37.0 33.0 37.0 33.0 40.0 62-63 35.188802065375775 37.0 33.0 37.0 33.0 40.0 64-65 34.923346107601176 37.0 33.0 37.0 27.0 40.0 66-67 34.66184193821796 37.0 33.0 37.0 27.0 38.5 68-69 34.41448124885323 37.0 33.0 37.0 27.0 37.0 70-71 34.17066169959308 37.0 33.0 37.0 27.0 37.0 72-73 33.97430490658892 37.0 33.0 37.0 27.0 37.0 74-75 33.72466263101114 37.0 33.0 37.0 27.0 37.0 76-77 30.92395413555232 33.0 30.0 33.0 24.5 37.0 78-79 33.48351200720174 35.0 33.0 37.0 27.0 37.0 80-81 33.976861009949374 37.0 33.0 37.0 27.0 37.0 82-83 33.95003462796529 37.0 33.0 37.0 27.0 37.0 84-85 33.828950253245345 37.0 33.0 37.0 27.0 37.0 86-87 33.73747031111471 37.0 33.0 37.0 27.0 37.0 88-89 33.590054026128264 37.0 33.0 37.0 27.0 37.0 90-91 33.482761398138116 37.0 33.0 37.0 27.0 37.0 92-93 33.375102957039985 37.0 33.0 37.0 27.0 37.0 94-95 33.25410675817169 37.0 33.0 37.0 27.0 37.0 96-97 33.14253483522967 33.0 33.0 37.0 27.0 37.0 98-99 32.987778375988015 33.0 33.0 37.0 27.0 37.0 100-101 30.85078600787935 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11277416787454086 2306 2 0.08845971149305853 2306 3 0.08416077810760214 2306 4 0.08860760059803852 2306 5 0.07061607204115461 2306 6 0.08957572244960232 2306 7 0.13628524151047827 2306 8 0.07427172109098024 2306 9 0.09438212103606958 2306 10-11 0.09586805640309137 2306 12-13 0.09255362186200244 2306 14-15 0.19976650092446846 2306 16-17 0.1957053872397836 2306 18-19 0.1420135328124701 2306 20-21 0.12536281644167957 2306 22-23 0.09906751106411349 2306 24-25 0.08861229087364819 2306 26-27 0.17187582030756943 2306 28-29 0.15981588841356853 2306 30-31 0.12158739636462457 2306 32-33 0.10125824949863471 2306 34-35 0.1556501588412189 2306 36-37 0.09398304748736308 2306 38-39 0.03712740069611442 2306 40-41 0.08716173145882067 2306 42-43 0.0700186989604461 2306 44-45 0.10215556068980902 2306 46-47 0.013062289188155773 2306 48-49 0.03545006684598917 2306 50-51 -0.10087615799010763 2306 52-53 -0.014003717436125385 2306 54-55 -0.08769110901498323 2306 56-57 -0.1642954113230033 2306 58-59 -0.11773207169413524 2306 60-61 -0.15007903030359415 2306 62-63 -0.1489080109152212 2306 64-65 -0.3416941617287037 2306 66-67 -0.36443718296888505 2306 68-69 -0.3690783720529538 2306 70-71 -0.46348683592025 2306 72-73 -0.36265273833833334 2306 74-75 -0.4386759522462427 2306 76-77 -0.7592646150871829 2306 78-79 -0.4378286413699328 2306 80-81 -0.4096371500308962 2306 82-83 -0.2933290153975108 2306 84-85 -0.3274512317323328 2306 86-87 -0.3463469645195332 2306 88-89 -0.5778906746329042 2306 90-91 -0.5943112132511104 2306 92-93 -0.48599539771444 2306 94-95 -0.6733289845358215 2306 96-97 -0.7210004390586775 2306 98-99 -0.5551219200460551 2306 100-101 -0.6942417629060564 2307 1 0.07101122896935408 2307 2 0.06881954730337725 2307 3 0.05851347154216313 2307 4 0.058305587080774046 2307 5 0.061951523034117884 2307 6 0.07787329243384278 2307 7 0.0981394619388638 2307 8 0.0788378414830504 2307 9 0.08455405217400624 2307 10-11 0.07616215826676864 2307 12-13 0.09614654991431593 2307 14-15 0.17856085429525592 2307 16-17 0.14526551508434693 2307 18-19 0.14484019235240453 2307 20-21 0.1647533622086712 2307 22-23 0.1564389035744682 2307 24-25 0.03844919348057374 2307 26-27 0.11391821334536445 2307 28-29 0.1217573072254936 2307 30-31 0.08835435337436337 2307 32-33 0.041126658761278634 2307 34-35 0.07498158503596386 2307 36-37 0.09535629129232603 2307 38-39 0.026683870601296178 2307 40-41 0.0013294083881447705 2307 42-43 0.008624937530811394 2307 44-45 -0.013185423687616549 2307 46-47 -0.006544810736038187 2307 48-49 0.014344078907981839 2307 50-51 -0.07232693561979886 2307 52-53 -0.09633981889422927 2307 54-55 -0.119562636387613 2307 56-57 -0.13580597085613988 2307 58-59 -0.10890744029865118 2307 60-61 -0.15578790495592187 2307 62-63 -0.1801537608140933 2307 64-65 -0.3224427819576121 2307 66-67 -0.2673559261316356 2307 68-69 -0.38167886174827004 2307 70-71 -0.39621786590287655 2307 72-73 -0.4104480254763061 2307 74-75 -0.4557933914209187 2307 76-77 -0.9255890804720046 2307 78-79 -0.46285117356154615 2307 80-81 -0.3135686865875513 2307 82-83 -0.14548814473644 2307 84-85 -0.1408076080934464 2307 86-87 -0.18767146413335922 2307 88-89 -0.2950517643428654 2307 90-91 -0.3566517669516216 2307 92-93 -0.38335433353019965 2307 94-95 -0.39024885010558563 2307 96-97 -0.37457110209575006 2307 98-99 -0.3696224388436349 2307 100-101 -0.5790041120835632 2201 1 0.11141221646028754 2201 2 0.0844158846076013 2201 3 0.08131630219859431 2201 4 0.06801656192105554 2201 5 0.05899378500172503 2201 6 0.09860851847895447 2201 7 0.12969453784075569 2201 8 0.1060781197599141 2201 9 0.08926332143432347 2201 10-11 0.10398506306476207 2201 12-13 0.11992862379298685 2201 14-15 0.21258643512855713 2201 16-17 0.18479498116010262 2201 18-19 0.1805709259774133 2201 20-21 0.2011131145708802 2201 22-23 0.18024037950470273 2201 24-25 0.16212086490888566 2201 26-27 0.15964973076915356 2201 28-29 0.12789387664954432 2201 30-31 0.18451168336104473 2201 32-33 0.14685543325678196 2201 34-35 0.1408144638480664 2201 36-37 0.12980257603279455 2201 38-39 0.12085143384729946 2201 40-41 0.09013295203322969 2201 42-43 0.07635378353463551 2201 44-45 0.04029789773195347 2201 46-47 0.0983749753769203 2201 48-49 0.10288779858332475 2201 50-51 0.06416873858623973 2201 52-53 0.004014839951743454 2201 54-55 0.05081680545578138 2201 56-57 -0.039226889692464795 2201 58-59 0.04552368052589628 2201 60-61 0.009854165886771682 2201 62-63 -0.027010377668815977 2201 64-65 -0.04457842732221451 2201 66-67 -0.030075573982934145 2201 68-69 -0.10762980755603024 2201 70-71 -0.22848048373130325 2201 72-73 -0.2306748718378202 2201 74-75 -0.2716913984737843 2201 76-77 -0.8521675650593323 2201 78-79 -0.11753389177574292 2201 80-81 -0.009949684012475757 2201 82-83 0.14420263763916097 2201 84-85 0.10837023915853194 2201 86-87 -0.03209410188856765 2201 88-89 -0.02274815986426404 2201 90-91 -0.038803013145759735 2201 92-93 -0.04139639019616226 2201 94-95 -0.0878528933314513 2201 96-97 -0.11157441462858486 2201 98-99 -0.03641144469316515 2201 100-101 -0.22236576996241197 1101 1 0.07748077917188567 1101 2 0.07467802284969594 1101 3 0.0671835631593396 1101 4 0.04551760452430642 1101 5 0.04615463655132146 1101 6 0.03618141524712115 1101 7 0.05110065069504088 1101 8 0.06407822178515232 1101 9 0.023761025077654097 1101 10-11 0.04232031360771771 1101 12-13 0.05287393107914795 1101 14-15 0.08131578356420022 1101 16-17 0.0748435851410818 1101 18-19 0.07557169817872733 1101 20-21 0.09587741874562283 1101 22-23 0.10131131666960158 1101 24-25 0.056764093001376636 1101 26-27 0.05472871355301123 1101 28-29 0.024928316828130903 1101 30-31 0.09342418374890116 1101 32-33 0.06091703073006016 1101 34-35 0.011526805097588522 1101 36-37 0.020297172288955778 1101 38-39 0.0482586728358001 1101 40-41 0.008739782535130303 1101 42-43 -0.03552605159818967 1101 44-45 -0.00344324408469987 1101 46-47 -0.007511946477883669 1101 48-49 -0.01897723061921397 1101 50-51 -0.035175179543941226 1101 52-53 -0.037633202434662394 1101 54-55 -0.01711816607794958 1101 56-57 -0.05257406509225149 1101 58-59 -0.09207579627045703 1101 60-61 -0.07502573358156184 1101 62-63 -0.12451390165902865 1101 64-65 -0.18630095076041187 1101 66-67 -0.22910742549028384 1101 68-69 -0.1775533990687208 1101 70-71 -0.2892241223614249 1101 72-73 -0.2159934403781847 1101 74-75 -0.3634877762623674 1101 76-77 -0.6496103516034317 1101 78-79 -0.05293759529180164 1101 80-81 -0.0110010698465004 1101 82-83 0.10360618268304478 1101 84-85 0.05279020377926713 1101 86-87 0.04265026967541985 1101 88-89 -0.0012296176541255477 1101 90-91 -0.026165171705237356 1101 92-93 0.028488827907317216 1101 94-95 -0.03406192831471344 1101 96-97 -0.16079326857666132 1101 98-99 -0.029503700749863526 1101 100-101 -0.223603176551034 2304 1 0.04264021843707155 2304 2 0.05362260183038359 2304 3 0.05004801969395345 2304 4 0.03953096240061171 2304 5 0.03985549339629557 2304 6 0.04888516747426053 2304 7 0.07288665898146007 2304 8 0.046811991081916915 2304 9 0.06077339879361432 2304 10-11 0.060793998640697566 2304 12-13 0.060671738792485996 2304 14-15 0.0874612388933329 2304 16-17 0.10981564625581086 2304 18-19 0.06021458984390904 2304 20-21 0.0695104901597361 2304 22-23 0.09840783687089072 2304 24-25 0.11475010035735522 2304 26-27 0.07486850480095342 2304 28-29 0.06451076420442092 2304 30-31 0.11051683744974383 2304 32-33 0.06412443877631802 2304 34-35 0.055044255663027286 2304 36-37 0.039462962491860765 2304 38-39 0.03334419013153678 2304 40-41 0.027700861738864546 2304 42-43 0.026768803494917393 2304 44-45 0.02681118349369882 2304 46-47 -0.010223844837753404 2304 48-49 0.0216859481650431 2304 50-51 -0.030158152667269178 2304 52-53 -0.030077170286403998 2304 54-55 -0.05742761017228304 2304 56-57 -0.01598456501080392 2304 58-59 0.03424284877481654 2304 60-61 -0.03101396819129576 2304 62-63 -9.944892144062578E-4 2304 64-65 -0.14542682224890058 2304 66-67 -0.13078525566304222 2304 68-69 -0.18470061161972495 2304 70-71 -0.1195187537510023 2304 72-73 -0.14527376352918964 2304 74-75 -0.17808539997099615 2304 76-77 -0.7951215370385114 2304 78-79 -0.18474204460635946 2304 80-81 0.003365040984881773 2304 82-83 -0.03557865413008443 2304 84-85 0.019205315306770387 2304 86-87 -0.018911103444374078 2304 88-89 -0.05968165084130561 2304 90-91 -0.0663180929111391 2304 92-93 0.052797077617242394 2304 94-95 -0.020538371827761637 2304 96-97 -0.13578898914319382 2304 98-99 -0.08400570253066775 2304 100-101 -0.10236980863629697 2202 1 0.07087335203348033 2202 2 0.02916802649569661 2202 3 0.03831896610245167 2202 4 0.0361015078158573 2202 5 0.06002307078549052 2202 6 0.050453705151262795 2202 7 0.0681220669035838 2202 8 0.05470000020819299 2202 9 0.04602276639717928 2202 10-11 0.021003809166344922 2202 12-13 0.040872636130096396 2202 14-15 0.028166640492877093 2202 16-17 0.01211295038402227 2202 18-19 0.015839386691588686 2202 20-21 0.04980197996686542 2202 22-23 0.04775732848077041 2202 24-25 -2.643344784587498E-4 2202 26-27 0.020992245542487353 2202 28-29 0.017526198405612092 2202 30-31 -0.006829066883497603 2202 32-33 -0.0749589584346424 2202 34-35 -0.06577382630417361 2202 36-37 -0.04056833659369374 2202 38-39 -0.024173821051000743 2202 40-41 0.009273890536860563 2202 42-43 -0.032881482669999684 2202 44-45 -0.027268626996111323 2202 46-47 -0.09489178468940196 2202 48-49 -0.048218692137545816 2202 50-51 -0.008672271047551305 2202 52-53 -0.08495058387937604 2202 54-55 -0.10281207388452174 2202 56-57 -0.10501175894775372 2202 58-59 -0.035164434241082176 2202 60-61 -0.12387322086610197 2202 62-63 -0.03682509886485974 2202 64-65 -0.14506368994545227 2202 66-67 -0.14213171332103514 2202 68-69 -0.13970771323066344 2202 70-71 -0.17994553201582875 2202 72-73 -0.1408711927920976 2202 74-75 -0.11765065436124189 2202 76-77 -0.5072822823994763 2202 78-79 -0.08778538738755515 2202 80-81 0.02112304929111275 2202 82-83 0.04653931056411409 2202 84-85 0.051628509911033404 2202 86-87 0.03837300108600772 2202 88-89 -0.0687347548235806 2202 90-91 -0.022662820789392413 2202 92-93 0.03207399711646275 2202 94-95 0.018046760810342732 2202 96-97 -0.051316322761913113 2202 98-99 0.029655394629692466 2202 100-101 0.016098707123152423 1102 1 0.05070778211208449 1102 2 0.04485948752888902 1102 3 0.034134253537978054 1102 4 0.0016974361678450123 1102 5 0.00536118626920512 1102 6 0.022978285384169794 1102 7 -0.005036890451229681 1102 8 0.025870423490687244 1102 9 0.02913539667015641 1102 10-11 0.024580713225454076 1102 12-13 0.034343571628234315 1102 14-15 0.0018780274870024982 1102 16-17 0.005420346970524292 1102 18-19 0.03583897045996309 1102 20-21 0.026425950295070777 1102 22-23 0.008474686142086796 1102 24-25 -0.043106033064574945 1102 26-27 0.012994510243736102 1102 28-29 -0.037439475022061686 1102 30-31 -0.0237819705253699 1102 32-33 -0.019402381523569545 1102 34-35 -0.0542659494966955 1102 36-37 -0.032443435009902544 1102 38-39 -0.054051724768108045 1102 40-41 -0.054741109849288705 1102 42-43 -0.1285357436281771 1102 44-45 -0.12233537951681939 1102 46-47 -0.12010823516636293 1102 48-49 -0.1317121442623801 1102 50-51 -0.1490337205767034 1102 52-53 -0.18218290933038617 1102 54-55 -0.1730680362772432 1102 56-57 -0.16474904156702053 1102 58-59 -0.0913477406447285 1102 60-61 -0.19655018700891702 1102 62-63 -0.1162511806408375 1102 64-65 -0.1628263195664985 1102 66-67 -0.19030173842531894 1102 68-69 -0.2741987923337277 1102 70-71 -0.27328924606570126 1102 72-73 -0.22706631917105113 1102 74-75 -0.23424587461798296 1102 76-77 -0.35921650075157885 1102 78-79 -0.14162728556426174 1102 80-81 -0.14673489235823212 1102 82-83 -0.12426572654067769 1102 84-85 -0.1182451261781523 1102 86-87 -0.14498170047590975 1102 88-89 -0.17853238099943525 1102 90-91 -0.21019955699181736 1102 92-93 -0.19550125304877497 1102 94-95 -0.31801127491737446 1102 96-97 -0.27881973034494933 1102 98-99 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0.13325707199564363 1213 58-59 0.15730215655715796 1213 60-61 0.12221834590319958 1213 62-63 0.21672618288003775 1213 64-65 0.2668163316219463 1213 66-67 0.25086957505043017 1213 68-69 0.3238520342299722 1213 70-71 0.36918973464502614 1213 72-73 0.39320319157678085 1213 74-75 0.39699560066033257 1213 76-77 0.8288114051756068 1213 78-79 0.29653283712487166 1213 80-81 0.24063472954528464 1213 82-83 0.14369887079158872 1213 84-85 0.21930917713478948 1213 86-87 0.30238031603864357 1213 88-89 0.31020700488630126 1213 90-91 0.2976101118812551 1213 92-93 0.4149796415455498 1213 94-95 0.4145550841907024 1213 96-97 0.5106691624441382 1213 98-99 0.5802250753222609 1213 100-101 0.8042392316141118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 46.0 8 485.0 9 1440.0 10 910.0 11 328.0 12 256.0 13 500.0 14 1170.0 15 2937.0 16 5466.0 17 8685.0 18 12230.0 19 17149.0 20 22460.0 21 29321.0 22 37636.0 23 49008.0 24 62718.0 25 80654.0 26 103800.0 27 134237.0 28 171916.0 29 222101.0 30 288763.0 31 384194.0 32 526358.0 33 765508.0 34 1238994.0 35 2354344.0 36 4986873.0 37 6444704.0 38 1114540.0 39 115.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.538777851101727 22.744340101516176 30.556936689335235 26.15994535804686 2 27.18186464736712 23.009439981348564 29.735388018582427 20.073307352701885 3 21.537902218093308 21.108737789034176 36.45157719409076 20.901782798781763 4 21.516251674472826 21.293611332555027 35.436447065668716 21.753689927303427 5 28.09644985615249 20.522891452668706 31.132242434876378 20.248416256302423 6 29.19897574427314 21.09539735688493 30.04952268363768 19.65610421520424 7 28.159544227072193 26.671205070496896 24.972869472943334 20.196381229487578 8 26.40443837855647 22.592913304443396 29.962558168400616 21.040090148599514 9 22.951075590695616 21.702727871912135 33.56938836478342 21.77680817260883 10-11 27.400145725219204 22.509179144455203 27.78968760585484 22.30098752447075 12-13 26.388019788517443 25.94486206417912 24.739042216751923 22.928075930551515 14-15 26.084081849214623 24.177296755448534 26.019936080242278 23.718685315094557 16-17 25.649686649295088 23.90165741759753 25.560262754074536 24.888393179032846 18-19 25.508353580393173 24.473714970025718 26.013861044611424 24.00407040496969 20-21 24.606264037183063 24.865160317652226 26.346432270077962 24.182143375086746 22-23 24.709645022322412 24.97653756739632 25.840131281598637 24.473686128682626 24-25 25.145010340145884 24.811441853728557 25.58617014599016 24.457377660135396 26-27 24.933301796337133 24.91220284319078 25.79720521069417 24.35729014977792 28-29 24.850318469250034 25.358809999351067 25.293242762256167 24.497628769142732 30-31 25.009973467103343 25.20827077110377 25.260742417156244 24.52101334463664 32-33 24.80081775171033 25.523762723424053 25.26400709979477 24.411412425070846 34-35 24.647208758413218 25.563626703454933 25.233196679553853 24.555967858577997 36-37 24.77120418721875 25.441330347676715 25.324939766150134 24.4625256989544 38-39 24.77100585946129 25.138427960524822 25.444493745895496 24.64607243411839 40-41 24.66044081183346 25.426172922062218 25.057900680681062 24.85548558542326 42-43 24.64301627590405 25.335858751252697 25.181822381689795 24.83930259115346 44-45 24.91513105845363 25.148239114653997 25.195033250921604 24.741596575970767 46-47 24.631917902469915 25.209374131489138 25.36017495307698 24.798533012963965 48-49 24.680440890099355 24.999486083400694 25.283763245544844 25.03630978095511 50-51 24.58690922151306 25.22874746259678 25.671817480386753 24.512525835503407 52-53 24.7449004407889 25.078706123173422 25.70802997145322 24.468363464584453 54-55 24.49454823416236 25.193668320267932 25.32686314378066 24.98492030178905 56-57 24.655977313781055 25.414188101845152 25.48250081378665 24.447333770587136 58-59 24.54319204895682 25.56286701898794 25.47303183492376 24.42090909713149 60-61 25.006469533616443 25.448503417059637 25.06571994666624 24.479307102657685 62-63 24.64452108210569 25.62948374303064 25.480779807183858 24.24521536767981 64-65 24.854077803895894 25.49932703336109 25.288168623174005 24.358426539569013 66-67 25.084404125547348 25.458507800523222 25.37090134332174 24.086186730607693 68-69 24.857308598985774 25.185435488003517 25.757973900380236 24.199282012630473 70-71 24.74227750332127 25.61740257953657 25.39924936307731 24.24107055406485 72-73 24.611176902353872 25.21567656178847 25.70905056365594 24.464095972201722 74-75 24.582361533734343 25.19749501099603 25.70638154566001 24.513761909609617 76-77 24.504867442147447 25.218898839322932 25.52462191476111 24.751611803768505 78-79 24.754807619698077 25.09029691265212 25.333323509521477 24.821571958128327 80-81 24.58452401119474 25.01033241251653 25.730784013472157 24.674359562816573 82-83 24.38216681020236 25.64783121440111 25.58682570452882 24.383176270867715 84-85 24.52276435033236 25.356040541105735 25.444876134815186 24.67631897374672 86-87 24.385143520202966 25.50124525123001 25.66919650320081 24.44441472536622 88-89 24.454128019833945 26.32055684585203 24.965642403661846 24.25967273065218 90-91 24.528042783445468 25.53320925767739 25.174499746590296 24.764248212286848 92-93 24.328645237394028 25.81559573190864 25.22278399415913 24.632975036538195 94-95 24.423165308291413 25.988173579180568 24.84211616747387 24.746544945054154 96-97 24.309951767684733 25.41365183555031 25.212470346670408 25.063926050094548 98-99 24.564243038989694 25.41887064283032 25.178677911694024 24.83820840648596 100-101 24.767022567047537 25.953289933158015 24.6090596802619 24.67062781953255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3396.0 1 2266.0 2 1529.5 3 1750.5 4 1790.5 5 1731.5 6 1468.0 7 1557.5 8 1643.5 9 1691.5 10 1772.0 11 1816.5 12 1974.5 13 2143.0 14 2275.0 15 2447.5 16 2702.5 17 2995.0 18 3242.0 19 3724.0 20 4316.0 21 4858.0 22 5788.0 23 6993.5 24 8357.0 25 10076.0 26 12201.0 27 14663.5 28 17159.0 29 19982.5 30 24277.0 31 31278.5 32 41932.5 33 55736.5 34 73967.0 35 102925.0 36 135356.0 37 173376.0 38 219218.5 39 268022.5 40 333378.5 41 423219.5 42 514377.0 43 599467.5 44 713425.0 45 847652.5 46 977013.5 47 1111480.5 48 1227301.5 49 1253554.5 50 1191783.5 51 1122781.0 52 1066709.5 53 1033895.5 54 989268.5 55 908622.5 56 829075.0 57 687463.0 58 521079.5 59 399208.0 60 306642.5 61 237822.5 62 171861.0 63 114198.5 64 72956.0 65 45268.5 66 27597.5 67 17882.0 68 12861.0 69 9812.0 70 7543.5 71 5569.5 72 4237.5 73 3171.0 74 2327.5 75 1706.0 76 1238.0 77 941.5 78 716.0 79 548.0 80 398.5 81 266.0 82 187.5 83 143.0 84 109.5 85 75.5 86 53.0 87 50.5 88 44.5 89 31.0 90 29.5 91 26.5 92 16.5 93 15.5 94 12.0 95 11.5 96 11.5 97 4.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15486228075138725 2 0.0 3 0.0 4 0.0687105670014028 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002073954762196047 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0032905350525360795 22-23 0.0 24-25 0.0 26-27 9.465204414067926E-4 28-29 5.742049215182481E-4 30-31 0.002624562221186148 32-33 0.0 34-35 0.0 36-37 2.6219402809052424E-5 38-39 4.7457119084384894E-4 40-41 6.449973091026896E-4 42-43 0.0 44-45 7.865820842715729E-4 46-47 0.008644537106144585 48-49 0.0031725477398953437 50-51 0.0021001741650050997 52-53 0.005078698324113455 54-55 0.006098633093385594 56-57 0.016347797651444188 58-59 0.02003424568639696 60-61 0.008523927853222944 62-63 0.003925044600515148 64-65 0.012629886333120555 66-67 0.029433901593442252 68-69 0.021248204036456088 70-71 0.015587434969981667 72-73 0.023560755364214512 74-75 0.017913095999144618 76-77 0.032456998737326 78-79 0.0028054761005686098 80-81 1.3109701404526212E-5 82-83 0.0013529211849471054 84-85 0.010694894405812486 86-87 0.012635130213682365 88-89 0.014362988858798921 90-91 0.010021055753619838 92-93 0.02192990850949145 94-95 0.023516182379439123 96-97 0.018251326295381395 98-99 0.021583812392411958 100-101 0.344520330970668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9069847E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.020635037472616 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56924326136001 16.640827259163956 2 10.862838379500364 4.78413198852056 3 4.271357958762489 2.8217404417293834 4 1.628819241905138 1.4347053627202349 5 1.0683169287008436 1.1762508595637466 6 0.7034658368571178 0.9294458672856508 7 0.5725684874881427 0.8825825187853866 8 0.4743835508229556 0.8356981632362117 9 0.36830488043627124 0.7299276619146405 >10 3.189639700632871 14.761687033633065 >50 0.6268373179130502 9.927497402807944 >100 0.5655257403749699 24.938671551475974 >500 0.07189995531613678 10.988112976010296 >1k 0.02656963306118975 8.814645437806776 >5k 2.2912686855929698E-4 0.3340754753461778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.34143278653468E-5 0.0 0.0 2 7.34143278653468E-5 0.0 0.0 3 7.865820842715728E-5 0.0 0.0 4 7.865820842715728E-5 0.0 0.0 5 7.865820842715728E-5 0.0 0.0 6 8.390208898896776E-5 0.0 0.0 7 8.390208898896776E-5 0.0 0.0 8 1.0487761123620971E-4 0.0 0.0 9 1.2060925292164116E-4 0.0 0.0 10-11 1.284750737643569E-4 0.0 0.0 12-13 1.6780417797793553E-4 0.0 0.0 14-15 2.4121850584328232E-4 0.0 0.0 16-17 3.2774253511315535E-4 0.0 0.0 18-19 4.2475432550664936E-4 0.0 0.0 20-21 5.243880561810485E-4 0.0 0.0 22-23 6.712167119117421E-4 0.0 0.0 24-25 8.495086510132987E-4 0.0 0.0 26-27 0.00108023939573296 0.0 0.0 28-29 0.001329323722418958 0.0 0.0 30-31 0.001609871332475819 0.0 2.6219402809052426E-6 32-33 0.001898284763375396 0.0 5.243880561810485E-6 34-35 0.002184076253994067 0.0 5.243880561810485E-6 36-37 0.002543282072478085 0.0 5.243880561810485E-6 38-39 0.003044072666130987 0.0 5.243880561810485E-6 40-41 0.00351602191669393 0.0 5.243880561810485E-6 42-43 0.004145287584111189 0.0 5.243880561810485E-6 44-45 0.004895162504450088 0.0 5.243880561810485E-6 46-47 0.005768268617991535 0.0 5.243880561810485E-6 48-49 0.006956007565241609 0.0 5.243880561810485E-6 50-51 0.00835612367524501 0.0 5.243880561810485E-6 52-53 0.009908312321540913 0.0 5.243880561810485E-6 54-55 0.011864279771096223 0.0 5.243880561810485E-6 56-57 0.014399696022731593 0.0 5.243880561810485E-6 58-59 0.01759321928487418 0.0 5.243880561810485E-6 60-61 0.02169131194392907 5.243880561810485E-6 5.243880561810485E-6 62-63 0.026607449970626404 5.243880561810485E-6 5.243880561810485E-6 64-65 0.03247010843873052 5.243880561810485E-6 5.243880561810485E-6 66-67 0.04031495375919901 5.243880561810485E-6 5.243880561810485E-6 68-69 0.05048546010883045 5.243880561810485E-6 5.243880561810485E-6 70-71 0.06275614062346699 5.243880561810485E-6 5.243880561810485E-6 72-73 0.07808400350563904 5.243880561810485E-6 5.243880561810485E-6 74-75 0.0974208130773152 5.243880561810485E-6 5.243880561810485E-6 76-77 0.12214308798597073 1.048776112362097E-5 5.243880561810485E-6 78-79 0.1535932616554291 1.048776112362097E-5 5.243880561810485E-6 80-81 0.19114206841827308 1.048776112362097E-5 5.243880561810485E-6 82-83 0.2382006525799604 1.048776112362097E-5 5.243880561810485E-6 84-85 0.29723625994482283 1.048776112362097E-5 5.243880561810485E-6 86-87 0.36905907005966015 1.048776112362097E-5 5.243880561810485E-6 88-89 0.4574053478247623 1.048776112362097E-5 7.865820842715729E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 156940 0.0 68.23904 1 TTTGTGT 168875 0.0 63.520267 2 TAACTTT 19285 0.0 58.33314 1 AACTTTG 19515 0.0 57.948086 2 TTGTGTT 192025 0.0 57.33472 3 TGTGTTT 206955 0.0 53.565865 4 ACTTTGT 42145 0.0 51.64611 1 GTGTTTG 229605 0.0 48.248253 5 TTGAGTC 20615 0.0 35.12629 4 TTTGAGG 71180 0.0 34.328907 8 GTTTGAG 183220 0.0 33.732834 7 TGTTTGA 339090 0.0 32.648903 6 TTTGAGT 68275 0.0 32.43059 3 TTGAGGG 37900 0.0 31.885761 9 TTTGAGC 45155 0.0 31.021612 8 TTGAGGC 13420 0.0 28.554148 9 GTTTGAC 66725 0.0 27.725445 7 GTTTGAA 30625 0.0 26.267065 2 TGAGTCT 5195 0.0 26.232792 5 TTGAGTG 33305 0.0 25.408861 4 >>END_MODULE