FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_7047.34100000035c46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_7047.34100000035c46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19069847
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1569400.068.239041
TTTGTGT1688750.063.5202672
TAACTTT192850.058.333141
AACTTTG195150.057.9480862
TTGTGTT1920250.057.334723
TGTGTTT2069550.053.5658654
ACTTTGT421450.051.646111
GTGTTTG2296050.048.2482535
TTGAGTC206150.035.126294
TTTGAGG711800.034.3289078
GTTTGAG1832200.033.7328347
TGTTTGA3390900.032.6489036
TTTGAGT682750.032.430593
TTGAGGG379000.031.8857619
TTTGAGC451550.031.0216128
TTGAGGC134200.028.5541489
GTTTGAC667250.027.7254457
GTTTGAA306250.026.2670652
TGAGTCT51950.026.2327925
TTGAGTG333050.025.4088614