FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_619.34100000035c2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_619.34100000035c2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17202182
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364910.21213006582537028No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT327660.190475836146833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273990.15927630576167603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1758700.060.3106961
TTTGTGT1928550.055.104132
ACTTTGT595550.052.6154861
TTGTGTT2102600.051.8894733
TGTGTTT2247950.048.80494
TAACTTT321050.048.6009521
AACTTTG324300.048.4500852
GTGTTTG2626750.041.7732625
TTGAGGG499200.033.692159
TTTGAGG861050.033.6565638
GTTTGAG2076000.031.9401727
TGTTTGA3525100.031.1087446
TTGAGTC241000.029.63124
TTTGAGT774900.028.2474883
TTTGACC204650.027.217058
GTTTGAC685700.027.0352527
TTGACCC121100.026.7028569
TTGAGGC148900.026.6922729
TTTGAGA261550.025.9074128
GTTTGAA194450.025.7048532
Produced by FastQC (version 0.11.2)