FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_30515.34100000035be7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_30515.34100000035be7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18290016
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1699800.071.495461
TTTGTGT1852700.065.6867452
TTGTGTT2075200.060.132043
TAACTTT186800.060.065121
AACTTTG196650.057.6454432
TGTGTTT2213800.056.6935424
GTGTTTG2396650.052.3644525
ACTTTGT415550.050.509051
TTTGAGG720050.038.203348
GTTTGAG1953950.036.5287557
TGTTTGA3450000.036.3380626
TTGAGGG372250.034.8885659
TTTGAGC537250.033.8783958
TTGAGGC145750.032.221689
GTTTGAC631250.031.925267
TTGAGTC258150.031.7287964
TTGAGGT222900.031.0178579
TTTGAGT740450.030.5100753
TTTGATG352200.027.976238
TTTGACC195800.027.9383188
Produced by FastQC (version 0.11.2)