##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l08n01_30515.34100000035be7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18290016 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74687949972269 33.0 33.0 33.0 33.0 33.0 2 32.77645344870119 33.0 33.0 33.0 33.0 33.0 3 32.792287825226616 33.0 33.0 33.0 33.0 33.0 4 36.71023130870962 37.0 37.0 37.0 37.0 37.0 5 36.72576366253589 37.0 37.0 37.0 37.0 37.0 6 36.67386704309061 37.0 37.0 37.0 37.0 37.0 7 36.62210213484778 37.0 37.0 37.0 37.0 37.0 8 36.638055428710395 37.0 37.0 37.0 37.0 37.0 9 36.62430842050657 37.0 37.0 37.0 37.0 37.0 10-11 36.60563908199971 37.0 37.0 37.0 37.0 37.0 12-13 36.57174471580561 37.0 37.0 37.0 37.0 37.0 14-15 38.658023645249955 40.0 37.0 40.0 37.0 40.0 16-17 38.646837979802754 40.0 37.0 40.0 37.0 40.0 18-19 38.60177536203358 40.0 37.0 40.0 37.0 40.0 20-21 38.54414539604558 40.0 37.0 40.0 37.0 40.0 22-23 38.49964502491414 40.0 37.0 40.0 37.0 40.0 24-25 38.46478146875323 40.0 37.0 40.0 37.0 40.0 26-27 38.35169397883523 40.0 37.0 40.0 37.0 40.0 28-29 38.29750479715272 40.0 37.0 40.0 37.0 40.0 30-31 38.228153162905926 40.0 37.0 40.0 37.0 40.0 32-33 38.141183064027935 40.0 37.0 40.0 37.0 40.0 34-35 38.0632352371917 40.0 37.0 40.0 37.0 40.0 36-37 37.961525402711516 40.0 37.0 40.0 35.0 40.0 38-39 37.84632041874649 40.0 37.0 40.0 33.0 40.0 40-41 37.72801699572051 40.0 37.0 40.0 33.0 40.0 42-43 37.58708384946192 40.0 37.0 40.0 33.0 40.0 44-45 37.38851480501712 40.0 37.0 40.0 33.0 40.0 46-47 37.18573977737363 37.0 37.0 40.0 33.0 40.0 48-49 36.961394183580815 37.0 37.0 40.0 33.0 40.0 50-51 36.745844235456104 37.0 37.0 40.0 33.0 40.0 52-53 36.487722181325594 37.0 37.0 40.0 33.0 40.0 54-55 36.22559504595294 37.0 37.0 40.0 33.0 40.0 56-57 35.9174722701172 37.0 37.0 40.0 33.0 40.0 58-59 35.63844014679921 37.0 33.0 37.0 33.0 40.0 60-61 35.33878218039831 37.0 33.0 37.0 33.0 40.0 62-63 35.04561051231448 37.0 33.0 37.0 30.0 40.0 64-65 34.76909317629902 37.0 33.0 37.0 27.0 38.5 66-67 34.504501444941326 37.0 33.0 37.0 27.0 37.0 68-69 34.254597726978474 37.0 33.0 37.0 27.0 37.0 70-71 34.023340602873176 37.0 33.0 37.0 27.0 37.0 72-73 33.84499144232569 37.0 33.0 37.0 27.0 37.0 74-75 33.610179263921914 37.0 33.0 37.0 27.0 37.0 76-77 30.760412566068833 33.0 30.0 33.0 24.5 37.0 78-79 33.36769128578127 35.0 33.0 37.0 27.0 37.0 80-81 33.886900044264586 37.0 33.0 37.0 27.0 37.0 82-83 33.87355844849999 37.0 33.0 37.0 27.0 37.0 84-85 33.75505598245513 37.0 33.0 37.0 27.0 37.0 86-87 33.66715174005315 37.0 33.0 37.0 27.0 37.0 88-89 33.501502650407744 37.0 33.0 37.0 27.0 37.0 90-91 33.40190902512059 37.0 33.0 37.0 27.0 37.0 92-93 33.29145127593108 37.0 33.0 37.0 27.0 37.0 94-95 33.17008137116993 35.0 33.0 37.0 27.0 37.0 96-97 33.06158078265213 33.0 33.0 37.0 27.0 37.0 98-99 32.914748215638525 33.0 33.0 37.0 27.0 37.0 100-101 30.7682208971277 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1052156644886324 2306 2 0.07981792122694031 2306 3 0.06599792678589012 2306 4 0.0668708204548949 2306 5 0.07192340840941824 2306 6 0.09792800373640631 2306 7 0.12699134981970417 2306 8 0.08004605257024622 2306 9 0.09829077273389686 2306 10-11 0.08481478469894199 2306 12-13 0.0562024028961261 2306 14-15 0.18761709756784484 2306 16-17 0.16757826813002907 2306 18-19 0.0998267140186968 2306 20-21 0.126725187807601 2306 22-23 0.11063247903452833 2306 24-25 0.09272825105598059 2306 26-27 0.09307864408146571 2306 28-29 0.07266138804373412 2306 30-31 0.071898187174682 2306 32-33 0.03004929424434266 2306 34-35 0.07214962788043522 2306 36-37 0.05339440289723285 2306 38-39 -0.02355651047693641 2306 40-41 0.03810153054183729 2306 42-43 -0.004073645985819496 2306 44-45 0.0322504050964838 2306 46-47 -0.004250115923881026 2306 48-49 -0.03992847925710663 2306 50-51 -0.19188537456596322 2306 52-53 -0.10033257948885677 2306 54-55 -0.1727738810588022 2306 56-57 -0.1890784853597154 2306 58-59 -0.17099537388946828 2306 60-61 -0.21065982300738284 2306 62-63 -0.13129215905559732 2306 64-65 -0.42441563933548565 2306 66-67 -0.4406421002760297 2306 68-69 -0.36912528148757673 2306 70-71 -0.5092331910602752 2306 72-73 -0.4604100866811933 2306 74-75 -0.4693567501795357 2306 76-77 -0.7996151057386811 2306 78-79 -0.46967691240637777 2306 80-81 -0.43926446434410593 2306 82-83 -0.2988573193941235 2306 84-85 -0.3166236275787355 2306 86-87 -0.31354033467851394 2306 88-89 -0.444448586989445 2306 90-91 -0.5711147312743989 2306 92-93 -0.5436900266189966 2306 94-95 -0.7277098986942434 2306 96-97 -0.6699285080511999 2306 98-99 -0.5473087535538141 2306 100-101 -0.7401037326516757 2307 1 0.06721473210353679 2307 2 0.05840297983979781 2307 3 0.05218143953545962 2307 4 0.071543695455766 2307 5 0.06690723816597455 2307 6 0.0697625134739539 2307 7 0.11607260186659119 2307 8 0.0744921883740659 2307 9 0.0948678471691693 2307 10-11 0.0688422492975036 2307 12-13 0.08900179756369653 2307 14-15 0.16615626563861952 2307 16-17 0.13982104999401912 2307 18-19 0.15112029371649527 2307 20-21 0.14758767739726153 2307 22-23 0.15141465083125638 2307 24-25 0.047195540806228564 2307 26-27 0.11342219889264271 2307 28-29 0.1522412510808664 2307 30-31 0.10972851699160913 2307 32-33 0.06159671292006408 2307 34-35 0.05473736233768989 2307 36-37 0.04330122807937897 2307 38-39 0.008968275299771733 2307 40-41 -0.009663453186185222 2307 42-43 -0.056121669302442 2307 44-45 -0.03730682420840736 2307 46-47 -0.05105833469048093 2307 48-49 -0.08533266444912613 2307 50-51 -0.10591991450367289 2307 52-53 -0.13309351902606892 2307 54-55 -0.1631091497755932 2307 56-57 -0.1831140470957493 2307 58-59 -0.14960910085143553 2307 60-61 -0.1944501994173038 2307 62-63 -0.23704693094820328 2307 64-65 -0.36774257102803176 2307 66-67 -0.33634897874929237 2307 68-69 -0.42161404183534756 2307 70-71 -0.4406704380706259 2307 72-73 -0.3770033544499256 2307 74-75 -0.4934042439272659 2307 76-77 -0.8895203713437994 2307 78-79 -0.4369838401251869 2307 80-81 -0.3059940910676531 2307 82-83 -0.27145889106081 2307 84-85 -0.3085432886211308 2307 86-87 -0.3308427731409793 2307 88-89 -0.3515833614130557 2307 90-91 -0.3898925145353118 2307 92-93 -0.3584847240740743 2307 94-95 -0.44941241751657657 2307 96-97 -0.4413484805217749 2307 98-99 -0.4751275935765804 2307 100-101 -0.6701913170943818 2201 1 0.09726008011422493 2201 2 0.06726317620071853 2201 3 0.05251059289882676 2201 4 0.048942105300341154 2201 5 0.060639131319440764 2201 6 0.07129675656662471 2201 7 0.10876977934442067 2201 8 0.09686794310107416 2201 9 0.06013227158646828 2201 10-11 0.09585545879258461 2201 12-13 0.10645580262414 2201 14-15 0.1778419059033638 2201 16-17 0.18447845495150972 2201 18-19 0.17244842762803358 2201 20-21 0.17302010893182995 2201 22-23 0.21829748777790314 2201 24-25 0.16173845539374554 2201 26-27 0.17652010653748818 2201 28-29 0.12043073433900275 2201 30-31 0.1774487194801253 2201 32-33 0.16191621815507773 2201 34-35 0.12084260360840915 2201 36-37 0.1373657869089513 2201 38-39 0.12581495164697998 2201 40-41 0.07400041017925219 2201 42-43 0.067453225504849 2201 44-45 0.06639786955542348 2201 46-47 0.10531920466915778 2201 48-49 0.09426266515907855 2201 50-51 0.07455237493483224 2201 52-53 0.02087085407612932 2201 54-55 0.04797635449355653 2201 56-57 0.03473078397913554 2201 58-59 0.0664114174533168 2201 60-61 -0.0037985607076436168 2201 62-63 0.04353845378573595 2201 64-65 -0.03092437021528127 2201 66-67 -0.0541262634366646 2201 68-69 -0.09706641714291919 2201 70-71 -0.16567724444742282 2201 72-73 -0.15839751550314674 2201 74-75 -0.27010440663143953 2201 76-77 -0.8406618508436168 2201 78-79 -0.12391747913078888 2201 80-81 -0.014539121863123228 2201 82-83 0.1714269160923365 2201 84-85 0.09502762908957152 2201 86-87 0.010933382671993286 2201 88-89 0.0124712047896125 2201 90-91 -0.04016537148546462 2201 92-93 0.008405394154905821 2201 94-95 -0.09568837733951341 2201 96-97 -0.10906496103420693 2201 98-99 -0.08439869980173142 2201 100-101 -0.21074125462645554 1101 1 0.07796811088850575 1101 2 0.07539522384129071 1101 3 0.06489750176268672 1101 4 0.040776231711575406 1101 5 0.04822773485478393 1101 6 0.05245597607164143 1101 7 0.04859837636337261 1101 8 0.04127987941763678 1101 9 0.05310789168341046 1101 10-11 0.06090936900402255 1101 12-13 0.09116565608024274 1101 14-15 0.10240978580539917 1101 16-17 0.078310842862372 1101 18-19 0.10678820646597842 1101 20-21 0.09138888538338108 1101 22-23 0.0948621295909291 1101 24-25 0.08080422530640874 1101 26-27 0.05053363282699053 1101 28-29 0.05867840707968952 1101 30-31 0.09757469855813383 1101 32-33 0.062047619609266746 1101 34-35 0.04022983931442781 1101 36-37 -0.0046575773636234885 1101 38-39 0.011239359087930723 1101 40-41 0.04070886083835745 1101 42-43 -0.0026839323248069036 1101 44-45 -0.013427150603817495 1101 46-47 0.033887935928554214 1101 48-49 0.058223486020260395 1101 50-51 0.04653532188552134 1101 52-53 -0.043735599335398945 1101 54-55 -0.013021211060618043 1101 56-57 -0.03776511600920429 1101 58-59 0.011632058063945294 1101 60-61 -0.049976279683370706 1101 62-63 -0.07429496100252919 1101 64-65 -0.09253364012691634 1101 66-67 -0.16079996964952414 1101 68-69 -0.1504746924183138 1101 70-71 -0.2165250147295339 1101 72-73 -0.21096971047148116 1101 74-75 -0.35195074446557584 1101 76-77 -0.635513694894577 1101 78-79 -0.06893544522509387 1101 80-81 0.05001451365095022 1101 82-83 0.15840730010972237 1101 84-85 0.0919926832213136 1101 86-87 0.04367611665383464 1101 88-89 -0.022714163110606478 1101 90-91 -0.051192170497650125 1101 92-93 0.0034401880194039336 1101 94-95 -0.04838754315925087 1101 96-97 -0.09589447450630928 1101 98-99 0.022848871716711017 1101 100-101 -0.16808750078120127 2304 1 0.04629605316425511 2304 2 0.06707065721617766 2304 3 0.0526581475415 2304 4 0.035214925278623355 2304 5 0.040639228539639305 2304 6 0.043674823059198786 2304 7 0.06176051242019298 2304 8 0.05122800296769725 2304 9 0.042265563894630986 2304 10-11 0.02915092332580116 2304 12-13 0.03431851377754214 2304 14-15 0.05487940806946767 2304 16-17 0.04588955916670301 2304 18-19 0.03358608361226345 2304 20-21 0.0781250742301296 2304 22-23 0.06531434510345235 2304 24-25 0.07811679246063363 2304 26-27 0.028403450641683037 2304 28-29 0.02179867508512956 2304 30-31 0.056853229669656 2304 32-33 0.039492298872538356 2304 34-35 0.00722960969530817 2304 36-37 0.004268912695991389 2304 38-39 0.02125324854879551 2304 40-41 0.012270486117429869 2304 42-43 0.024445785006577125 2304 44-45 -0.043427855684264216 2304 46-47 -0.05438846371852435 2304 48-49 -0.010661922183878403 2304 50-51 -0.0419938771805306 2304 52-53 -0.043199457410814546 2304 54-55 -0.07860066551554468 2304 56-57 -0.0654930753816032 2304 58-59 0.006687431837704594 2304 60-61 -0.08122365123082176 2304 62-63 -0.0059165087601869 2304 64-65 -0.127402878902096 2304 66-67 -0.15624057941307967 2304 68-69 -0.26448526488123747 2304 70-71 -0.24586759272827408 2304 72-73 -0.25883462704112503 2304 74-75 -0.259395380051302 2304 76-77 -0.932712669220912 2304 78-79 -0.26446314490325307 2304 80-81 -0.05956088440751728 2304 82-83 -0.03677024601065426 2304 84-85 -0.07138227169721745 2304 86-87 -0.05774657033911268 2304 88-89 -0.11623564372962392 2304 90-91 -0.17514019670917236 2304 92-93 -0.05202668131109078 2304 94-95 -0.09318691969342296 2304 96-97 -0.12365534803234368 2304 98-99 -0.11615073456136571 2304 100-101 -0.14046168447899277 2202 1 0.06551840449791513 2202 2 0.03417149738596237 2202 3 0.025439770295029973 2202 4 0.02506147152367788 2202 5 0.05181153447873754 2202 6 0.03469132320346091 2202 7 0.05552626394795368 2202 8 0.05708032697204146 2202 9 0.04763742563747542 2202 10-11 0.03650237255295963 2202 12-13 0.027964443581161902 2202 14-15 0.017975634316869105 2202 16-17 -0.005080747534115915 2202 18-19 0.058160794000158944 2202 20-21 0.025682271782237365 2202 22-23 0.061176219912283614 2202 24-25 -0.013759613046588015 2202 26-27 0.01908029760870278 2202 28-29 0.037857460711755664 2202 30-31 -0.009371589173497341 2202 32-33 -0.08073383996002548 2202 34-35 -0.022896105519713217 2202 36-37 -0.049382765333604084 2202 38-39 -0.09496432997351434 2202 40-41 -0.03508535868351714 2202 42-43 -0.057983744841919815 2202 44-45 -0.02585133731580669 2202 46-47 -0.07644895932283902 2202 48-49 -0.015476573609355171 2202 50-51 -0.014492366163374015 2202 52-53 -0.03686982971083097 2202 54-55 -0.03631797783042856 2202 56-57 -0.058975673124727734 2202 58-59 -0.02991241546529011 2202 60-61 -0.13839450290506505 2202 62-63 -0.07156733649511438 2202 64-65 -0.12465715907178065 2202 66-67 -0.08473545902848656 2202 68-69 -0.10429872478505331 2202 70-71 -0.09535839443138627 2202 72-73 -0.08308219952314744 2202 74-75 -0.07355593055061149 2202 76-77 -0.4899880155693026 2202 78-79 -0.022775742445809044 2202 80-81 0.0374989605613365 2202 82-83 0.08383209693781168 2202 84-85 0.08338658743723926 2202 86-87 0.07769391808811577 2202 88-89 0.007077387351571929 2202 90-91 0.07481431339246569 2202 92-93 0.052634519852787776 2202 94-95 0.05459756068886179 2202 96-97 -0.04699210765759432 2202 98-99 0.07164826117770673 2202 100-101 0.021141907252879122 1102 1 0.06461513321647772 1102 2 0.04968268551042598 1102 3 0.03017490571745185 1102 4 -0.008052428566152514 1102 5 0.013675346396027521 1102 6 0.02649912552496403 1102 7 -0.01820665109106301 1102 8 0.013649870103961348 1102 9 0.02401230862032122 1102 10-11 0.01533306395742784 1102 12-13 0.031241999264096876 1102 14-15 0.04103689873345928 1102 16-17 0.032040209658298124 1102 18-19 0.02600873214053223 1102 20-21 -0.0015557701553916559 1102 22-23 0.014942646685334182 1102 24-25 -0.012049532791188255 1102 26-27 0.028604061667984126 1102 28-29 -0.04701418444461325 1102 30-31 -0.010606173431909838 1102 32-33 -0.009605229974191332 1102 34-35 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0.1327922388564602 1213 56-57 0.11402502700220651 1213 58-59 0.14477261080516257 1213 60-61 0.11380209306616251 1213 62-63 0.19348502982121119 1213 64-65 0.22729984304877604 1213 66-67 0.22370558837042154 1213 68-69 0.29879637499613665 1213 70-71 0.3267103093709096 1213 72-73 0.3464816955450871 1213 74-75 0.4142007293631522 1213 76-77 0.8309241285439342 1213 78-79 0.2403819142430237 1213 80-81 0.18978037824236793 1213 82-83 0.13239133796599845 1213 84-85 0.2311125955708917 1213 86-87 0.26845584381997156 1213 88-89 0.28721823758724696 1213 90-91 0.28960611580913564 1213 92-93 0.38838507262049404 1213 94-95 0.38490098588809474 1213 96-97 0.48201703026109044 1213 98-99 0.54837606640492 1213 100-101 0.7777394377389832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 32.0 8 286.0 9 911.0 10 655.0 11 231.0 12 220.0 13 431.0 14 1245.0 15 3006.0 16 5345.0 17 8383.0 18 12029.0 19 16278.0 20 21766.0 21 28307.0 22 37751.0 23 48672.0 24 62474.0 25 81306.0 26 104757.0 27 133870.0 28 171911.0 29 221792.0 30 285223.0 31 376325.0 32 514116.0 33 747984.0 34 1200029.0 35 2274894.0 36 4915835.0 37 6161821.0 38 852075.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.211897253521236 21.996006678972343 30.467116268484524 27.324979799021897 2 27.25082361874369 22.448640832244216 30.49947031210908 19.801065236903018 3 21.338969851092532 20.588735406245682 37.351957483252065 20.720337259409725 4 21.390608894749978 20.74221746171507 36.156040266712175 21.71113337682278 5 29.232943262597473 19.985220351912215 30.848103139986314 19.933733245503994 6 29.299476829325897 20.585241696890808 30.74157507571344 19.373706398069853 7 29.05300902962578 26.18356922159062 24.754068011750235 20.009353737033365 8 26.545001382174842 21.82749867468678 30.764281452788232 20.863218490350143 9 22.820707209878876 21.02618171575137 34.3736112642001 21.779499810169657 10-11 27.772167885532205 21.90879726756436 28.084593791236685 22.23444105566675 12-13 26.710419498812904 25.88655745298419 24.513499605467814 22.889523442735097 14-15 26.391934266213873 23.580184402244374 26.053194814044993 23.97468651749676 16-17 25.840439396007085 23.387915570986927 25.434584092217307 25.33706094078868 18-19 25.83045307341448 24.028945628041004 26.20283109648455 23.937770202059966 20-21 24.569408089935287 24.6830967876122 26.548451489733104 24.199043632719402 22-23 24.74808934010774 24.730120520397577 25.814190102403412 24.707600037091275 24-25 25.144187954783636 24.42052265017155 25.74137988725652 24.69390950778829 26-27 24.99717739049601 24.52700309183041 25.90245455215972 24.573364965513864 28-29 25.07022187016365 24.879548183077794 25.202923626751968 24.847306320006588 30-31 25.461301934646976 24.74072778046982 25.09513580528732 24.70283447959589 32-33 25.249513177025108 25.241730242335493 24.928447301522315 24.58030927911709 34-35 25.226093295927132 25.26189424875298 24.890336345249782 24.621676110070105 36-37 25.212275063309843 25.126791227395618 25.087737115471388 24.57319659382316 38-39 25.16858603911805 24.81390704901427 25.230199215207023 24.78730769666066 40-41 24.88484276650298 25.19395625489051 24.867305750345224 25.05389522826128 42-43 24.76512048977978 24.995956810535322 25.243690328100314 24.995232371584585 44-45 25.243430602967592 24.881008543338773 25.153490073887102 24.72207077980653 46-47 24.628894971304522 25.029678614681934 25.21684895595962 25.124577458053928 48-49 25.00878172434656 24.79454838425977 25.08120344110731 25.115466450286362 50-51 24.73429570544439 24.944863471309567 25.71180210129265 24.60903872195339 52-53 24.764680314260243 25.113953743893326 25.61582891720343 24.505537024642997 54-55 24.686193257902794 24.825184489398733 25.230607476705778 25.25801477599269 56-57 24.73683531298798 25.208054583828492 25.389024394778637 24.666085708404886 58-59 24.577902510928045 25.360667709832263 25.569916954069477 24.49151282517022 60-61 25.310690695097875 25.000120294529125 24.908308229140953 24.780880781232042 62-63 24.75521714924104 25.291751512391997 25.468094840582534 24.48493649778443 64-65 24.954617171687676 25.225984557753783 25.44300956804209 24.37638870251645 66-67 25.192512955587375 25.2659952429571 25.280236674656326 24.261255126799195 68-69 25.03537165868901 25.098126702737446 25.78717714107531 24.079324497498234 70-71 24.845370783252463 25.64165393486547 25.204463216881017 24.308512065001047 72-73 24.96041733407956 25.18124737569331 25.40549263903507 24.452842651192057 74-75 24.796460919342675 25.21619302859338 25.32343168287172 24.66391436919222 76-77 24.808595159819014 24.961467994877093 25.269198990973113 24.96073785433078 78-79 24.928371618791026 24.949203200212054 25.074922614228967 25.047502566767953 80-81 24.656657504743233 24.827359985788963 25.513912390258735 25.00207011920907 82-83 24.411061612899097 25.507884897866973 25.324080002300743 24.75697348693319 84-85 24.74416184469492 25.194665780989368 25.15909342128427 24.902078953031445 86-87 24.448471297792867 25.500044879687035 25.374294265896136 24.67718955662396 88-89 24.67505197413113 26.418511414076406 24.585922607198167 24.320514004594294 90-91 24.71011914674269 25.400521586364228 24.838480277483136 25.05087898940995 92-93 24.437930435477366 25.768143213140043 24.858302577436106 24.935623773946485 94-95 24.653381388097564 25.904122126650815 24.55752834275157 24.88496814250005 96-97 24.475703518510564 25.243653015674216 24.917467172863812 25.363176292951405 98-99 24.738695346038615 25.1787846605095 25.06559258744906 25.016927406002825 100-101 25.176264165801634 25.763206076407265 24.356669740574368 24.703860017216737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1379.0 1 1281.5 2 1431.5 3 1710.0 4 1883.0 5 2010.5 6 2019.0 7 2101.5 8 2277.0 9 2385.5 10 2501.5 11 2729.0 12 2904.5 13 3089.0 14 3325.0 15 3661.5 16 4022.0 17 4313.0 18 4861.0 19 5477.5 20 6139.5 21 6945.5 22 8201.5 23 9944.5 24 11579.0 25 13483.5 26 15867.5 27 18392.5 28 21384.0 29 26088.5 30 32058.5 31 38670.5 32 47248.0 33 59734.5 34 74783.5 35 91907.5 36 111702.5 37 135364.0 38 163900.0 39 199850.5 40 254250.0 41 340037.5 42 419351.5 43 459955.0 44 537226.5 45 665224.5 46 810857.5 47 993173.0 48 1206957.0 49 1301868.0 50 1256739.0 51 1211479.5 52 1173105.5 53 1141287.5 54 1055278.0 55 940307.5 56 841219.0 57 674272.5 58 504249.0 59 390941.0 60 299430.0 61 219734.5 62 155628.5 63 104400.5 64 60886.0 65 36478.5 66 23448.0 67 15990.5 68 12171.0 69 9324.5 70 7077.5 71 5387.5 72 4117.0 73 3033.5 74 2232.5 75 1551.0 76 1017.0 77 750.0 78 550.0 79 363.5 80 228.5 81 159.0 82 110.0 83 79.0 84 46.5 85 30.5 86 24.5 87 12.5 88 11.0 89 11.5 90 9.0 91 7.0 92 4.0 93 5.0 94 4.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15595393683635925 2 0.0 3 0.0 4 0.06990152441638105 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021842517797688093 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003275010803708427 22-23 0.0 24-25 0.0 26-27 0.0011098951471666292 28-29 7.107702912889743E-4 30-31 0.002752868012799989 32-33 0.0 34-35 0.0 36-37 4.647344212274063E-5 38-39 5.303439865771577E-4 40-41 7.189714869576932E-4 42-43 0.0 44-45 8.337882263197583E-4 46-47 0.008720604727737799 48-49 0.0033488215647268977 50-51 0.0022799323959038637 52-53 0.0049562559157958095 54-55 0.005973204178716956 56-57 0.016336781772088117 58-59 0.020172207613159004 60-61 0.008583918133259151 62-63 0.0037916861308377207 64-65 0.012367403068428153 66-67 0.029182587921191543 68-69 0.020975924788693458 70-71 0.01532256724105654 72-73 0.023149241640903977 74-75 0.017676310397978876 76-77 0.032066675064691026 78-79 0.002613447686431767 80-81 3.553851456444871E-5 82-83 0.0013231262345533214 84-85 0.010609613463432727 86-87 0.012206112886943347 88-89 0.013958435028159626 90-91 0.00967467715719877 92-93 0.021632020442190977 94-95 0.023028957437762766 96-97 0.017504085288935777 98-99 0.02100326210758919 100-101 0.3411861422100451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.8290016E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.747897703321893 #Duplication Level Percentage of deduplicated Percentage of total 1 79.33823579953545 21.22131015128005 2 10.185088927473233 5.4485943346258106 3 3.2224288077685252 2.5857958831929015 4 1.4634444845123884 1.5657625346491202 5 1.0669408075519764 1.4269211787949958 6 0.6724184989843361 1.0791468734792566 7 0.46491537174860154 0.8704856162963425 8 0.2766900813389117 0.5920702392941619 9 0.3231829245434852 0.7780017424634584 >10 2.1672878940505584 11.902783729363476 >50 0.3509420383879768 6.673635140579154 >100 0.385060723686181 23.33480579227838 >500 0.05311697162320601 9.597611008985151 >1k 0.02992497034754873 12.306243548911523 >5k 3.0113306446718634E-4 0.5487542298789619 >10k+ 2.0565383296408993E-5 0.06807799592712806 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2575166692035698E-4 0.0 5.467463779145956E-6 2 1.2575166692035698E-4 0.0 5.467463779145956E-6 3 1.3121913069950294E-4 0.0 5.467463779145956E-6 4 1.366865944786489E-4 0.0 5.467463779145956E-6 5 1.4215405825779485E-4 0.0 5.467463779145956E-6 6 1.4215405825779485E-4 5.467463779145956E-6 5.467463779145956E-6 7 1.5308898581608676E-4 5.467463779145956E-6 5.467463779145956E-6 8 1.6949137715352463E-4 5.467463779145956E-6 1.0934927558291912E-5 9 1.6949137715352463E-4 5.467463779145956E-6 1.0934927558291912E-5 10-11 1.8862750038053548E-4 5.467463779145956E-6 1.6402391337437868E-5 12-13 2.4056840628242207E-4 5.467463779145956E-6 1.6402391337437868E-5 14-15 3.444502180861952E-4 5.467463779145956E-6 1.6402391337437868E-5 16-17 4.647344212274062E-4 1.0934927558291912E-5 1.6402391337437868E-5 18-19 6.041547475956282E-4 1.3668659447864891E-5 1.6402391337437868E-5 20-21 7.545100015221419E-4 1.9136123227010845E-5 1.6402391337437868E-5 22-23 9.240013786756666E-4 2.733731889572978E-5 1.6402391337437868E-5 24-25 0.0011591023211789428 3.007105078530276E-5 1.6402391337437868E-5 26-27 0.0014078719231300837 3.2804782674875736E-5 1.6402391337437868E-5 28-29 0.0017167836266518302 3.2804782674875736E-5 1.6402391337437868E-5 30-31 0.0020639675766275984 3.2804782674875736E-5 1.6402391337437868E-5 32-33 0.0024712936281739723 3.2804782674875736E-5 1.6402391337437868E-5 34-35 0.002870418484051627 3.2804782674875736E-5 1.6402391337437868E-5 36-37 0.0034089636662975034 3.2804782674875736E-5 1.6402391337437868E-5 38-39 0.0040951303705803205 3.827224645402169E-5 1.6402391337437868E-5 40-41 0.004841439176433744 3.827224645402169E-5 1.6402391337437868E-5 42-43 0.0057572393594406916 3.827224645402169E-5 1.6402391337437868E-5 44-45 0.006856199579049029 3.827224645402169E-5 1.6402391337437868E-5 46-47 0.007985230849442668 4.373971023316765E-5 1.6402391337437868E-5 48-49 0.009469647265480795 4.373971023316765E-5 1.6402391337437868E-5 50-51 0.011375058392513161 4.373971023316765E-5 1.6402391337437868E-5 52-53 0.013720600353766776 4.373971023316765E-5 1.6402391337437868E-5 54-55 0.01665116093938901 4.9207174012313604E-5 1.6402391337437868E-5 56-57 0.0203444327222021 4.9207174012313604E-5 1.6402391337437868E-5 58-59 0.0249070312458994 4.9207174012313604E-5 1.9136123227010845E-5 60-61 0.03021320484356055 5.467463779145956E-5 2.1869855116583825E-5 62-63 0.03723889579976311 5.467463779145956E-5 2.4603587006156802E-5 64-65 0.045934896940494754 5.7408369681032534E-5 3.007105078530276E-5 66-67 0.057058451999167197 6.014210157060552E-5 3.2804782674875736E-5 68-69 0.07077085115726525 6.014210157060552E-5 3.2804782674875736E-5 70-71 0.08765437930726797 6.28758334601785E-5 3.2804782674875736E-5 72-73 0.10919892032899262 6.560956534975147E-5 3.2804782674875736E-5 74-75 0.1360277650932618 6.560956534975147E-5 3.2804782674875736E-5 76-77 0.16933828816770854 6.834329723932444E-5 3.2804782674875736E-5 78-79 0.21095115499078843 7.107702912889743E-5 3.2804782674875736E-5 80-81 0.26104679186721325 7.107702912889743E-5 3.2804782674875736E-5 82-83 0.3235645064498577 7.381076101847041E-5 3.553851456444871E-5 84-85 0.39997778022720154 8.74794204663353E-5 3.827224645402169E-5 86-87 0.4924544625876763 8.74794204663353E-5 3.827224645402169E-5 88-89 0.606153105606906 8.74794204663353E-5 3.827224645402169E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 169980 0.0 71.49546 1 TTTGTGT 185270 0.0 65.686745 2 TTGTGTT 207520 0.0 60.13204 3 TAACTTT 18680 0.0 60.06512 1 AACTTTG 19665 0.0 57.645443 2 TGTGTTT 221380 0.0 56.693542 4 GTGTTTG 239665 0.0 52.364452 5 ACTTTGT 41555 0.0 50.50905 1 TTTGAGG 72005 0.0 38.20334 8 GTTTGAG 195395 0.0 36.528755 7 TGTTTGA 345000 0.0 36.338062 6 TTGAGGG 37225 0.0 34.888565 9 TTTGAGC 53725 0.0 33.878395 8 TTGAGGC 14575 0.0 32.22168 9 GTTTGAC 63125 0.0 31.92526 7 TTGAGTC 25815 0.0 31.728796 4 TTGAGGT 22290 0.0 31.017857 9 TTTGAGT 74045 0.0 30.510075 3 TTTGATG 35220 0.0 27.97623 8 TTTGACC 19580 0.0 27.938318 8 >>END_MODULE