##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l08n01_30455.34100000035ba4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14181806 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74631037824096 33.0 33.0 33.0 33.0 33.0 2 32.775603614941566 33.0 33.0 33.0 33.0 33.0 3 32.790877269086884 33.0 33.0 33.0 33.0 33.0 4 36.709076051385836 37.0 37.0 37.0 37.0 37.0 5 36.72441704533259 37.0 37.0 37.0 37.0 37.0 6 36.67024947316301 37.0 37.0 37.0 37.0 37.0 7 36.617314113590325 37.0 37.0 37.0 37.0 37.0 8 36.633630582733964 37.0 37.0 37.0 37.0 37.0 9 36.61963483353249 37.0 37.0 37.0 37.0 37.0 10-11 36.60208135691604 37.0 37.0 37.0 37.0 37.0 12-13 36.565616466619275 37.0 37.0 37.0 37.0 37.0 14-15 38.64104490641037 40.0 37.0 40.0 37.0 40.0 16-17 38.631497568081244 40.0 37.0 40.0 37.0 40.0 18-19 38.58523445462447 40.0 37.0 40.0 37.0 40.0 20-21 38.52709862904626 40.0 37.0 40.0 37.0 40.0 22-23 38.48304112325327 40.0 37.0 40.0 37.0 40.0 24-25 38.4469357428807 40.0 37.0 40.0 37.0 40.0 26-27 38.33259854210388 40.0 37.0 40.0 37.0 40.0 28-29 38.28086419317822 40.0 37.0 40.0 37.0 40.0 30-31 38.20900504491459 40.0 37.0 40.0 37.0 40.0 32-33 38.121876966868676 40.0 37.0 40.0 37.0 40.0 34-35 38.04444137791759 40.0 37.0 40.0 37.0 40.0 36-37 37.94342226229861 40.0 37.0 40.0 35.0 40.0 38-39 37.82555398797586 40.0 37.0 40.0 33.0 40.0 40-41 37.70622137265169 40.0 37.0 40.0 33.0 40.0 42-43 37.56627840629042 40.0 37.0 40.0 33.0 40.0 44-45 37.36669282459512 40.0 37.0 40.0 33.0 40.0 46-47 37.16251452741632 37.0 37.0 40.0 33.0 40.0 48-49 36.938355488715615 37.0 37.0 40.0 33.0 40.0 50-51 36.723541486888195 37.0 37.0 40.0 33.0 40.0 52-53 36.465657758962436 37.0 37.0 40.0 33.0 40.0 54-55 36.202877475548604 37.0 37.0 40.0 33.0 40.0 56-57 35.895211195245516 37.0 37.0 40.0 33.0 40.0 58-59 35.61604132083036 37.0 33.0 37.0 33.0 40.0 60-61 35.315332475990715 37.0 33.0 37.0 33.0 40.0 62-63 35.01939178268268 37.0 33.0 37.0 30.0 40.0 64-65 34.74169266594114 37.0 33.0 37.0 27.0 38.5 66-67 34.4752327030845 37.0 33.0 37.0 27.0 37.0 68-69 34.223738041544216 37.0 33.0 37.0 27.0 37.0 70-71 33.988281534805935 37.0 33.0 37.0 27.0 37.0 72-73 33.81045002307887 37.0 33.0 37.0 27.0 37.0 74-75 33.575514218710936 37.0 33.0 37.0 27.0 37.0 76-77 30.740663741980395 33.0 30.0 33.0 24.5 37.0 78-79 33.34756476008768 35.0 33.0 37.0 27.0 37.0 80-81 33.86839761452103 37.0 33.0 37.0 27.0 37.0 82-83 33.8545496603183 37.0 33.0 37.0 27.0 37.0 84-85 33.73632875107726 37.0 33.0 37.0 27.0 37.0 86-87 33.64695385058857 37.0 33.0 37.0 27.0 37.0 88-89 33.48703835745603 37.0 33.0 37.0 27.0 37.0 90-91 33.385944533439535 37.0 33.0 37.0 27.0 37.0 92-93 33.27754109737505 37.0 33.0 37.0 27.0 37.0 94-95 33.15912483219697 35.0 33.0 37.0 27.0 37.0 96-97 33.04282899512234 33.0 33.0 37.0 27.0 37.0 98-99 32.89080777159129 33.0 33.0 37.0 27.0 37.0 100-101 30.73877893266908 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07874724209897721 2306 2 0.08227413991462385 2306 3 0.08740486059408425 2306 4 0.08563217211229812 2306 5 0.06724084258145524 2306 6 0.08573193773167276 2306 7 0.12302072297251243 2306 8 0.05664066549131519 2306 9 0.10460927286285937 2306 10-11 0.08838260320726476 2306 12-13 0.035417138419560956 2306 14-15 0.14125116601859844 2306 16-17 0.155230353776453 2306 18-19 0.08362445706935517 2306 20-21 0.08587091713633299 2306 22-23 0.08111252059050145 2306 24-25 0.08568019595904985 2306 26-27 0.1302001487217339 2306 28-29 0.11034913547275238 2306 30-31 0.0222897653349321 2306 32-33 0.007404635054086839 2306 34-35 0.047592969408988495 2306 36-37 -0.007712224527331557 2306 38-39 -0.03572153006957279 2306 40-41 -0.026851515200498 2306 42-43 -0.04376264152924847 2306 44-45 -0.003230776356154763 2306 46-47 -0.07365894226074943 2306 48-49 -0.052881934318492085 2306 50-51 -0.26060406518526946 2306 52-53 -0.12037742787575212 2306 54-55 -0.15971120284827123 2306 56-57 -0.2760582178318316 2306 58-59 -0.25305480279360637 2306 60-61 -0.26187473146469387 2306 62-63 -0.22944974908931925 2306 64-65 -0.5370829397480961 2306 66-67 -0.5355671327342293 2306 68-69 -0.4990585802442169 2306 70-71 -0.6501175617586981 2306 72-73 -0.5928401508604324 2306 74-75 -0.5944885580238548 2306 76-77 -0.8823307688612161 2306 78-79 -0.5844062938308028 2306 80-81 -0.5591563061150282 2306 82-83 -0.4428054523678071 2306 84-85 -0.4348142414818952 2306 86-87 -0.46591577100608816 2306 88-89 -0.6638664488122004 2306 90-91 -0.7367235863154704 2306 92-93 -0.6295875753983964 2306 94-95 -0.8076574552197542 2306 96-97 -0.7352859659940165 2306 98-99 -0.6107501310673413 2306 100-101 -0.8486106286460071 2307 1 0.07339678479387146 2307 2 0.06650075792500587 2307 3 0.04888903712718218 2307 4 0.06764374770624215 2307 5 0.05328285160919677 2307 6 0.085724960729614 2307 7 0.10146686363136581 2307 8 0.08175422865872406 2307 9 0.08636301085400078 2307 10-11 0.06839011282866636 2307 12-13 0.11115962848667493 2307 14-15 0.17976325180581654 2307 16-17 0.13713356780720432 2307 18-19 0.08500152727668109 2307 20-21 0.14451669972547165 2307 22-23 0.13352511495133257 2307 24-25 0.022076661609155224 2307 26-27 0.0845710380527791 2307 28-29 0.09342693091452503 2307 30-31 0.11292993019152675 2307 32-33 0.03650561719172174 2307 34-35 0.05189139447536917 2307 36-37 0.010819004409292177 2307 38-39 0.02121141408824201 2307 40-41 -0.046263139010513044 2307 42-43 -0.032949440792179985 2307 44-45 -0.05102253963146097 2307 46-47 -0.09457403214571514 2307 48-49 -0.041158483991630135 2307 50-51 -0.11411966439138865 2307 52-53 -0.16781912630231233 2307 54-55 -0.1297456798285168 2307 56-57 -0.17149519619191267 2307 58-59 -0.14978107818335928 2307 60-61 -0.20879030475622784 2307 62-63 -0.23701967173180805 2307 64-65 -0.3348663243914629 2307 66-67 -0.30863948673734143 2307 68-69 -0.4084761313912466 2307 70-71 -0.49938774865513835 2307 72-73 -0.43229905320358597 2307 74-75 -0.5210602990475053 2307 76-77 -0.9925200885661383 2307 78-79 -0.5133190059285582 2307 80-81 -0.34603063411136503 2307 82-83 -0.2793961994925951 2307 84-85 -0.2644934582386611 2307 86-87 -0.36782667531277724 2307 88-89 -0.364582198895981 2307 90-91 -0.40311153628537255 2307 92-93 -0.4222365561954433 2307 94-95 -0.4234871671567859 2307 96-97 -0.5329604284222356 2307 98-99 -0.5619298362142544 2307 100-101 -0.6931274837124946 2201 1 0.10167805542087649 2201 2 0.05495655881433947 2201 3 0.06369718167382388 2201 4 0.06169048860088111 2201 5 0.07166515979015031 2201 6 0.09010388336652397 2201 7 0.0846555498220809 2201 8 0.07364824145943771 2201 9 0.07146510713646848 2201 10-11 0.08789120282966678 2201 12-13 0.08664830271746382 2201 14-15 0.143476077842287 2201 16-17 0.114016194800314 2201 18-19 0.14840120779610544 2201 20-21 0.19163975716156045 2201 22-23 0.17258024629932578 2201 24-25 0.12852068375579506 2201 26-27 0.15588068582681558 2201 28-29 0.11832520882754238 2201 30-31 0.14898384370331286 2201 32-33 0.14721654205254708 2201 34-35 0.0972202233783932 2201 36-37 0.09789459094130848 2201 38-39 0.0872197205117331 2201 40-41 0.03220933834499107 2201 42-43 0.01375215436674182 2201 44-45 0.02660202986469784 2201 46-47 0.06455364000986918 2201 48-49 0.04406814717241758 2201 50-51 0.0693757427320989 2201 52-53 0.005503609600182813 2201 54-55 0.010532849921034426 2201 56-57 -0.058418245845686556 2201 58-59 -8.380431488674844E-4 2201 60-61 -0.06239065013806311 2201 62-63 -0.08073651186523279 2201 64-65 -0.13545136141831193 2201 66-67 -0.05289140850268126 2201 68-69 -0.16143893003444987 2201 70-71 -0.24764138372252376 2201 72-73 -0.29325084775438626 2201 74-75 -0.37414397715392766 2201 76-77 -0.8243876438963404 2201 78-79 -0.16571350190097434 2201 80-81 -0.1045476693754992 2201 82-83 0.11635975833205237 2201 84-85 0.05257876888927626 2201 86-87 -0.028016074921794143 2201 88-89 -0.07254878186699898 2201 90-91 -0.03925557898591592 2201 92-93 -0.056746343406530286 2201 94-95 -0.16071855209543173 2201 96-97 -0.2602032707848494 2201 98-99 -0.17088923379653664 2201 100-101 -0.37769835967844756 1101 1 0.06652856866096357 1101 2 0.05416403476778697 1101 3 0.06802090399012428 1101 4 0.0350672872218496 1101 5 0.07439480646126384 1101 6 0.0549125847864147 1101 7 0.08301063480460869 1101 8 0.063522178821529 1101 9 0.003943768854135499 1101 10-11 0.07450951982788467 1101 12-13 0.0914782806522183 1101 14-15 0.08849020833697807 1101 16-17 0.07616438159272576 1101 18-19 0.05240887076242018 1101 20-21 0.06723248722634878 1101 22-23 0.1087963145818307 1101 24-25 0.05854907778278573 1101 26-27 0.05662750195173061 1101 28-29 0.0284556902146349 1101 30-31 0.06755556142790908 1101 32-33 0.04886373771476116 1101 34-35 0.05215563361886666 1101 36-37 0.030433594717671042 1101 38-39 0.018119748449628048 1101 40-41 0.005388481088644426 1101 42-43 0.006468820368226602 1101 44-45 -0.04543070879052635 1101 46-47 0.013942807327467222 1101 48-49 -0.027344778761737132 1101 50-51 -0.02136374682159925 1101 52-53 -0.06742143130887257 1101 54-55 -0.054896947082582415 1101 56-57 -0.04664156657845808 1101 58-59 -0.04184356040860138 1101 60-61 -0.04534259315234834 1101 62-63 -0.05180415768870006 1101 64-65 -0.16227792028650612 1101 66-67 -0.20770988818688352 1101 68-69 -0.21064716620281132 1101 70-71 -0.28876924806976945 1101 72-73 -0.19835773397112888 1101 74-75 -0.3504171945925023 1101 76-77 -0.6749994743608134 1101 78-79 -0.15034670770825187 1101 80-81 -0.0455497656681203 1101 82-83 0.09100777882744637 1101 84-85 0.049435992926511574 1101 86-87 -0.013319220120941111 1101 88-89 -0.027316707259508632 1101 90-91 -0.05167725309318172 1101 92-93 0.013973836688386143 1101 94-95 -0.12417834717950171 1101 96-97 -0.2226469911245843 1101 98-99 -0.10861029003913103 1101 100-101 -0.3208316709655463 2304 1 0.03436312515591311 2304 2 0.06867816220395895 2304 3 0.05775445204944418 2304 4 0.031728390508703797 2304 5 0.04077450174164454 2304 6 0.043558388770264855 2304 7 0.07870893880085106 2304 8 0.05496138482124735 2304 9 0.034423192432313954 2304 10-11 0.04523224421141947 2304 12-13 0.036952946388190355 2304 14-15 0.05480080642985996 2304 16-17 0.06340539682461355 2304 18-19 0.06016402707615498 2304 20-21 0.09085010523062209 2304 22-23 0.06768406298098029 2304 24-25 0.06483495421033325 2304 26-27 0.047245527230771245 2304 28-29 0.04442586072369892 2304 30-31 0.015697298107014035 2304 32-33 0.032186930916580536 2304 34-35 0.05372305885374118 2304 36-37 0.016014597294358168 2304 38-39 -0.013870313667823098 2304 40-41 0.02420295657123006 2304 42-43 0.006655777579020139 2304 44-45 -0.032398535872559364 2304 46-47 -0.017792682505920254 2304 48-49 -0.019348318919348628 2304 50-51 -0.06862928312232697 2304 52-53 -0.06096100398876558 2304 54-55 -0.10254886800738916 2304 56-57 -0.06757009274413406 2304 58-59 -0.048053261275534 2304 60-61 -0.03598100389046266 2304 62-63 -0.004542007252375413 2304 64-65 -0.17688263556648565 2304 66-67 -0.15395085808652453 2304 68-69 -0.22449845638069377 2304 70-71 -0.2134072403917031 2304 72-73 -0.14900017427117973 2304 74-75 -0.17583057797155277 2304 76-77 -0.8479317576349565 2304 78-79 -0.22464264723144822 2304 80-81 -0.0029444117502350764 2304 82-83 -0.007260776999942209 2304 84-85 -0.014591846442762346 2304 86-87 -0.04032390993922519 2304 88-89 -0.14902956316432636 2304 90-91 -0.12112434092527025 2304 92-93 0.004878580798042265 2304 94-95 -0.03003032444510012 2304 96-97 -0.1603090111246459 2304 98-99 -0.09842224960726043 2304 100-101 -0.07104954175718348 2202 1 0.0532249065052639 2202 2 0.029838605677568353 2202 3 0.04236047477917282 2202 4 0.0466381212000897 2202 5 0.04016254335797953 2202 6 0.05919658433989383 2202 7 0.04364181276754664 2202 8 0.05412304565125936 2202 9 0.03524365817480657 2202 10-11 0.026264616975112176 2202 12-13 0.03953626122159193 2202 14-15 0.013385973922432015 2202 16-17 -0.03265675788183131 2202 18-19 0.008701335443802805 2202 20-21 0.013500130207930283 2202 22-23 0.03375347384407945 2202 24-25 -0.029447705054955975 2202 26-27 0.008283921412129303 2202 28-29 0.01756640892961059 2202 30-31 0.009656277142362057 2202 32-33 -0.05149576228068753 2202 34-35 -0.013021467160676536 2202 36-37 -0.06467430278281228 2202 38-39 -0.09437347016540087 2202 40-41 0.0034921673515384555 2202 42-43 0.013238292765088033 2202 44-45 -0.05302882882534021 2202 46-47 -0.0810149521405421 2202 48-49 -0.07146561735161328 2202 50-51 0.00915897994732262 2202 52-53 -0.030278009445282805 2202 54-55 -0.09036895918605126 2202 56-57 -0.16378286669242215 2202 58-59 -0.06214659652940924 2202 60-61 -0.1809774426130346 2202 62-63 -0.11987973769628013 2202 64-65 -0.13454516068984645 2202 66-67 -0.15406385095017328 2202 68-69 -0.1752102114598202 2202 70-71 -0.17882043429011674 2202 72-73 -0.10079564805372598 2202 74-75 -0.12020956100440827 2202 76-77 -0.5250011641404306 2202 78-79 -0.05868400612718716 2202 80-81 0.03640539940677456 2202 82-83 0.05114220364130517 2202 84-85 0.054998547034102785 2202 86-87 0.022877343753023638 2202 88-89 -0.04582044971314758 2202 90-91 -0.031550443915669746 2202 92-93 -0.03803156803338226 2202 94-95 -0.1157273337669622 2202 96-97 -0.08571824796831606 2202 98-99 -0.016192655291213498 2202 100-101 0.057524347638555184 1102 1 0.0679276481754556 1102 2 0.041522237717472876 1102 3 0.02786709265316034 1102 4 -3.21385674496355E-4 1102 5 0.02077176799548397 1102 6 0.03220473280310898 1102 7 -0.005560231097177848 1102 8 0.025924311081567453 1102 9 0.023132356232338225 1102 10-11 0.043491555381336866 1102 12-13 0.02878396139939099 1102 14-15 -0.013740788761403167 1102 16-17 -0.0064160586728760904 1102 18-19 0.026072874253458167 1102 20-21 0.031244783707592205 1102 22-23 -0.009304999676913894 1102 24-25 -0.03870327210335489 1102 26-27 0.0038848647563440863 1102 28-29 -0.06419672782235608 1102 30-31 0.0013842255584606278 1102 32-33 0.0026733121549966654 1102 34-35 0.0031215052686661693 1102 36-37 -0.028451252789295722 1102 38-39 -0.035848593883549995 1102 40-41 -0.03678770491675465 1102 42-43 -0.09469548069767342 1102 44-45 -0.1382238420045141 1102 46-47 -0.11897139443535565 1102 48-49 -0.12363073874971775 1102 50-51 -0.1253601438252545 1102 52-53 -0.16849131325524525 1102 54-55 -0.15786870498293126 1102 56-57 -0.11459024355965397 1102 58-59 -0.06429313603722875 1102 60-61 -0.22226974147569933 1102 62-63 -0.13939179659120526 1102 64-65 -0.23682976203132 1102 66-67 -0.22985171504245017 1102 68-69 -0.24044920535965986 1102 70-71 -0.21842948778805749 1102 72-73 -0.26534338847438477 1102 74-75 -0.18304338162007383 1102 76-77 -0.4202011154838061 1102 78-79 -0.21048968072857832 1102 80-81 -0.2149790901569375 1102 82-83 -0.11206920259604658 1102 84-85 -0.10622260091140134 1102 86-87 -0.134331131808743 1102 88-89 -0.11697027041044095 1102 90-91 -0.19533852873501445 1102 92-93 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1213 54-55 0.16045420956119472 1213 56-57 0.13988375784868623 1213 58-59 0.15390193242844674 1213 60-61 0.11402507024197206 1213 62-63 0.18069189538579167 1213 64-65 0.24193752990439066 1213 66-67 0.19895894071256492 1213 68-69 0.3550467542828457 1213 70-71 0.3671515503675309 1213 72-73 0.4313392707788992 1213 74-75 0.4506682164492801 1213 76-77 0.8597137150452347 1213 78-79 0.3239818588754346 1213 80-81 0.24746171638427938 1213 82-83 0.14237200941612826 1213 84-85 0.25722899547226064 1213 86-87 0.34765647419944656 1213 88-89 0.3447382339031222 1213 90-91 0.33911935576238506 1213 92-93 0.4501292287075387 1213 94-95 0.426302334384026 1213 96-97 0.5561994110243305 1213 98-99 0.5522871180963378 1213 100-101 0.8236511501909973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 22.0 8 282.0 9 857.0 10 569.0 11 208.0 12 172.0 13 381.0 14 991.0 15 2322.0 16 4356.0 17 6628.0 18 9408.0 19 12994.0 20 17208.0 21 22562.0 22 29390.0 23 38097.0 24 49047.0 25 63553.0 26 82004.0 27 104810.0 28 135023.0 29 173138.0 30 223504.0 31 294417.0 32 402380.0 33 584633.0 34 939171.0 35 1777685.0 36 3830145.0 37 4737115.0 38 638701.0 39 31.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.81511236988204 21.29219785189724 31.034918181301997 27.857771596918724 2 27.64296028305563 21.963866943321605 31.22986592821817 19.1633068454046 3 20.952796844069084 20.08316148168999 38.860092995208085 20.10394867903284 4 21.004404690763163 20.16005292713339 37.60816182008148 21.227380562021974 5 29.813593557830366 19.42525514733455 31.424693018646565 19.33645827618852 6 29.654805600922764 20.055569791322768 31.512312324678536 18.777312283075936 7 29.69490627639385 26.48527980145829 24.366685032921758 19.453128889226097 8 26.500912507194073 21.45880432999859 31.650559879327076 20.389723283480258 9 22.545901417633267 20.58920422406004 35.40472912970323 21.460165228603465 10-11 28.26963099332806 21.501672815767158 28.206323064492995 22.02237312641179 12-13 26.885454504172458 25.981313663436094 24.38177831511727 22.751453517274175 14-15 26.553014475025254 23.4726169572479 26.040343521833538 23.93402504589331 16-17 25.95830178469512 23.224827641839127 25.367262815469342 25.449607757996407 18-19 25.92382803713434 23.965822829617046 26.247979277110407 23.86236985613821 20-21 24.53041694501091 24.7020722068356 26.60965062481267 24.157860223340823 22-23 24.765639862793215 24.71045295641472 25.79650645340939 24.727400727382676 24-25 25.163811999684665 24.37781901684454 25.778307078802232 24.68006190466856 26-27 25.024799646077955 24.50642569618466 25.888630479914404 24.580144177822984 28-29 25.10568536195685 24.863743588752786 25.129109886404454 24.90146116288591 30-31 25.52303715871158 24.75737012474522 25.006502343505595 24.713090373037605 32-33 25.273808568527873 25.258094772978843 24.87321783981532 24.59487881867796 34-35 25.304330069104036 25.27193997717921 24.77939692589223 24.644333027824526 36-37 25.226444304648666 25.166889093791596 25.03709243929816 24.569574162261578 38-39 25.199466158181416 24.785994006795644 25.18559972482904 24.828940110193898 40-41 24.87008965918341 25.189628129313657 24.782067796648413 25.15821441485452 42-43 24.757185368351532 24.9947256364951 25.192803370741355 25.05528562441201 44-45 25.272541527484872 24.874080610409532 25.11298722324529 24.740390638860298 46-47 24.592813949409745 25.019119488747865 25.20020899038028 25.187857571462107 48-49 25.037736245538678 24.72799793192823 25.057670911732792 25.176594910800304 50-51 24.73440848716661 24.93429911231921 25.70827547188132 24.62301692863286 52-53 24.771039001343123 25.05594583561659 25.643889233909267 24.52912592913102 54-55 24.69789631987934 24.811012983664792 25.180565497993097 25.31052519846277 56-57 24.727731418508995 25.219153067254336 25.37123206200964 24.681883452227034 58-59 24.599462131638386 25.39444784777191 25.53840436805757 24.46768565253213 60-61 25.383295948310185 24.953412550303355 24.879106753636723 24.784184747749734 62-63 24.768271942768486 25.313409694105143 25.473998182674368 24.444320180452 64-65 24.997002825524532 25.20277824674743 25.454117772172708 24.34610115555533 66-67 25.23912451071156 25.27449367588817 25.265031568981318 24.22135024441895 68-69 25.064817718536908 25.060607207217565 25.855237190198142 24.019337884047385 70-71 24.853117573960972 25.648840394104965 25.214163831641933 24.28387820029213 72-73 25.00736328412628 25.135183972077073 25.413659287019684 24.44379345677696 74-75 24.80966068594583 25.19771627380595 25.30647000980233 24.686153030445897 76-77 24.82979484814011 24.88526055108299 25.274779532912266 25.010165067864637 78-79 24.976759248306635 24.884991465081402 25.06866453512591 25.06958475148605 80-81 24.661391784951462 24.745637067444807 25.55441645889454 25.038554688709187 82-83 24.399120734745733 25.50679441376681 25.34366064905097 24.750424202436484 84-85 24.74834129225329 25.163307362120847 25.171170374482486 24.91718097114338 86-87 24.421091351555177 25.50767142683962 25.42729815409454 24.643939067510658 88-89 24.707306622897402 26.499495215504776 24.49919034548656 24.29400781611126 90-91 24.710572406870263 25.355954059697627 24.834496572445726 25.098976960986384 92-93 24.416761853417306 25.74937283594946 24.870639645442974 24.963225665190265 94-95 24.646374867642937 25.882919374492495 24.55740567251705 24.913300085347515 96-97 24.4743065525868 25.141002722464645 24.932313932738673 25.452376792209886 98-99 24.761624396571808 25.072744275088848 25.145292835158795 25.02033849318055 100-101 25.210442616421215 25.708804552214872 24.3721532162693 24.708599615094624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1135.0 1 1025.5 2 1192.0 3 1448.5 4 1624.5 5 1696.5 6 1591.5 7 1635.5 8 1754.0 9 1837.0 10 1920.0 11 2092.5 12 2237.0 13 2406.0 14 2597.5 15 2807.5 16 3051.5 17 3287.0 18 3658.5 19 4115.0 20 4516.5 21 5136.5 22 6091.5 23 7358.0 24 8677.5 25 10045.0 26 11755.0 27 13844.5 28 16053.0 29 19543.5 30 24250.0 31 29039.0 32 35580.5 33 45214.5 34 56552.0 35 69626.5 36 85418.0 37 103736.0 38 125327.5 39 153287.0 40 195269.5 41 261486.5 42 323704.5 43 355290.5 44 415434.0 45 519316.5 46 635898.0 47 775885.5 48 939871.5 49 1014479.0 50 981270.5 51 946176.5 52 915625.0 53 888396.0 54 818568.0 55 725513.0 56 646262.0 57 518578.0 58 388995.5 59 302311.5 60 231351.0 61 168891.0 62 118921.5 63 79960.5 64 46627.5 65 27725.5 66 17854.0 67 12134.0 68 9228.0 69 6982.5 70 5357.0 71 4158.0 72 3124.0 73 2223.0 74 1616.5 75 1165.5 76 778.5 77 547.5 78 383.0 79 265.0 80 190.5 81 126.5 82 75.0 83 49.5 84 39.5 85 29.0 86 20.0 87 11.5 88 9.0 89 9.5 90 6.5 91 4.0 92 2.5 93 3.5 94 3.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15486038943136016 2 0.0 3 0.0 4 0.06958916233940868 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0023128224994757368 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0033528874954290027 22-23 0.0 24-25 0.0 26-27 0.0010717957924399756 28-29 6.205133535178806E-4 30-31 0.0026830151251540178 32-33 0.0 34-35 0.0 36-37 5.2884660811183005E-5 38-39 5.429491843281456E-4 40-41 7.192313870320889E-4 42-43 0.0 44-45 8.038468443299816E-4 46-47 0.00880705884708901 48-49 0.0033846182919157124 50-51 0.002118912076501399 52-53 0.004787824625439101 54-55 0.006145197586259465 56-57 0.01645065515633199 58-59 0.020251299446629012 60-61 0.008577891983573883 62-63 0.0037230801211072836 64-65 0.012579497984953399 66-67 0.02963303827453288 68-69 0.021192646409068067 70-71 0.015389436296054255 72-73 0.02362181516232841 74-75 0.017790399896881963 76-77 0.03227022002698387 78-79 0.002527886786774548 80-81 2.11538643244732E-5 82-83 0.001357372960820364 84-85 0.010506419281155024 86-87 0.012121164257923145 88-89 0.014049691555504285 90-91 0.009586226183040438 92-93 0.021855467491234896 94-95 0.023290404621244994 96-97 0.018040720624721562 98-99 0.021344249103393463 100-101 0.33988971503347315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4181806E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.111962003154378 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8410849442498 21.64648461299122 2 9.743340718344374 5.283221666790791 3 3.1705770601625503 2.578816943495999 4 1.461329953646801 1.5847808870937365 5 0.88773642037424 1.203413804900134 6 0.683083293875696 1.1111836985128443 7 0.40875531143570376 0.7757510930562636 8 0.3097116944972617 0.6717513354513853 9 0.27063557368622804 0.6603715251434611 >10 2.349911536189869 13.252620866290263 >50 0.36572063152741335 7.03099020520156 >100 0.43346076503573433 25.034544742569665 >500 0.050980824772167796 9.526790292180737 >1k 0.023295739154025295 8.983254411690265 >5k 3.493846951481949E-4 0.579225185987996 >10k+ 2.6148353061743386E-5 0.0767987286437818 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0576932162236601E-4 0.0 0.0 2 1.0576932162236601E-4 0.0 0.0 3 1.1987189783868147E-4 0.0 0.0 4 1.269231859468392E-4 0.0 0.0 5 1.269231859468392E-4 0.0 0.0 6 1.3397447405499695E-4 0.0 0.0 7 1.480770502713124E-4 7.051288108157734E-6 0.0 8 1.480770502713124E-4 7.051288108157734E-6 0.0 9 1.7628220270394336E-4 1.4102576216315468E-5 0.0 10-11 2.0096171108249542E-4 1.4102576216315468E-5 0.0 12-13 2.432694397314418E-4 1.4102576216315468E-5 0.0 14-15 2.820515243263093E-4 1.4102576216315468E-5 0.0 16-17 3.8076955784051763E-4 1.4102576216315468E-5 0.0 18-19 5.076927437873569E-4 1.4102576216315468E-5 0.0 20-21 6.698723702749847E-4 1.4102576216315468E-5 0.0 22-23 8.320519967626127E-4 1.4102576216315468E-5 0.0 24-25 0.001008334199466556 1.4102576216315468E-5 0.0 26-27 0.0011740394700082628 1.7628220270394333E-5 0.0 28-29 0.0014314114859560198 2.11538643244732E-5 0.0 30-31 0.0017099373662282505 2.11538643244732E-5 0.0 32-33 0.002087181280014689 2.467950837855207E-5 0.0 34-35 0.0024503226175848127 2.8205152432630935E-5 0.0 36-37 0.0029650666494803273 2.8205152432630935E-5 0.0 38-39 0.00357852871489005 3.173079648670981E-5 0.0 40-41 0.004220195932732404 3.525644054078867E-5 0.0 42-43 0.004971158116251202 3.525644054078867E-5 0.0 44-45 0.005937184587068812 3.525644054078867E-5 0.0 46-47 0.007093595836806681 4.230772864894641E-5 3.525644054078867E-6 48-49 0.008458020085735202 4.935901675710414E-5 7.051288108157734E-6 50-51 0.010002252181421745 4.935901675710414E-5 7.051288108157734E-6 52-53 0.012075330885220118 4.935901675710414E-5 7.051288108157734E-6 54-55 0.014515076570642695 4.935901675710414E-5 7.051288108157734E-6 56-57 0.017310912305527237 4.935901675710414E-5 1.4102576216315468E-5 58-59 0.0209705308336611 4.935901675710414E-5 1.7628220270394333E-5 60-61 0.025938163305858224 4.935901675710414E-5 2.8205152432630935E-5 62-63 0.031910604333467825 4.935901675710414E-5 2.8205152432630935E-5 64-65 0.03919458494919477 4.935901675710414E-5 2.8205152432630935E-5 66-67 0.04832247740520495 4.935901675710414E-5 2.8205152432630935E-5 68-69 0.05943530746366153 4.935901675710414E-5 2.8205152432630935E-5 70-71 0.07366833250997792 4.935901675710414E-5 2.8205152432630935E-5 72-73 0.09221322023443276 4.935901675710414E-5 2.8205152432630935E-5 74-75 0.11572926607513881 4.935901675710414E-5 3.173079648670981E-5 76-77 0.14380044403371472 4.935901675710414E-5 3.525644054078867E-5 78-79 0.17913797438774723 4.935901675710414E-5 3.525644054078867E-5 80-81 0.22208384461048192 4.935901675710414E-5 3.525644054078867E-5 82-83 0.27399190201868506 4.935901675710414E-5 3.525644054078867E-5 84-85 0.33962529172941724 5.2884660811183005E-5 3.525644054078867E-5 86-87 0.41874779559105524 5.641030486526187E-5 3.525644054078867E-5 88-89 0.5159744816703882 5.641030486526187E-5 3.525644054078867E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 148545 0.0 74.24807 1 TTTGTGT 162135 0.0 68.08838 2 TAACTTT 15585 0.0 63.818153 1 TTGTGTT 181120 0.0 62.286594 3 AACTTTG 16620 0.0 60.262436 2 TGTGTTT 192545 0.0 58.931236 4 GTGTTTG 208805 0.0 54.34296 5 ACTTTGT 32245 0.0 46.44502 1 TTTGAGG 62610 0.0 39.332157 8 GTTTGAG 171240 0.0 37.48852 7 TGTTTGA 305355 0.0 37.133854 6 TTGAGGG 32235 0.0 36.41495 9 TTTGAGC 45290 0.0 35.364944 8 TTGAGTC 22310 0.0 34.15953 4 TTGAGGC 12455 0.0 33.664795 9 GTTTGAC 54825 0.0 33.31981 7 TTGAGGT 19140 0.0 32.50037 9 TTTGAGT 65150 0.0 32.37262 3 TTTGATG 31045 0.0 29.91825 8 TTGAGCC 27675 0.0 29.735134 9 >>END_MODULE