FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_30455.34100000035ba4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_30455.34100000035ba4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14181806
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1485450.074.248071
TTTGTGT1621350.068.088382
TAACTTT155850.063.8181531
TTGTGTT1811200.062.2865943
AACTTTG166200.060.2624362
TGTGTTT1925450.058.9312364
GTGTTTG2088050.054.342965
ACTTTGT322450.046.445021
TTTGAGG626100.039.3321578
GTTTGAG1712400.037.488527
TGTTTGA3053550.037.1338546
TTGAGGG322350.036.414959
TTTGAGC452900.035.3649448
TTGAGTC223100.034.159534
TTGAGGC124550.033.6647959
GTTTGAC548250.033.319817
TTGAGGT191400.032.500379
TTTGAGT651500.032.372623
TTTGATG310450.029.918258
TTGAGCC276750.029.7351349
Produced by FastQC (version 0.11.2)