##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l08n01_30429.34100000035b60.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14865804 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.744429161046384 33.0 33.0 33.0 33.0 33.0 2 32.77364359169541 33.0 33.0 33.0 33.0 33.0 3 32.78897878648205 33.0 33.0 33.0 33.0 33.0 4 36.70650938220361 37.0 37.0 37.0 37.0 37.0 5 36.72124797286443 37.0 37.0 37.0 37.0 37.0 6 36.669859564945156 37.0 37.0 37.0 37.0 37.0 7 36.61393921243681 37.0 37.0 37.0 37.0 37.0 8 36.6304800601434 37.0 37.0 37.0 37.0 37.0 9 36.61580039666876 37.0 37.0 37.0 37.0 37.0 10-11 36.59673873004111 37.0 37.0 37.0 37.0 37.0 12-13 36.55899199935638 37.0 37.0 37.0 37.0 37.0 14-15 38.628958514453714 40.0 37.0 40.0 37.0 40.0 16-17 38.618005894602135 40.0 37.0 40.0 37.0 40.0 18-19 38.56696032047779 40.0 37.0 40.0 37.0 40.0 20-21 38.50504937371702 40.0 37.0 40.0 37.0 40.0 22-23 38.44883724418807 40.0 37.0 40.0 37.0 40.0 24-25 38.4054194108842 40.0 37.0 40.0 37.0 40.0 26-27 38.28817069026337 40.0 37.0 40.0 37.0 40.0 28-29 38.22785646171576 40.0 37.0 40.0 37.0 40.0 30-31 38.15879467400485 40.0 37.0 40.0 37.0 40.0 32-33 38.07279586088987 40.0 37.0 40.0 37.0 40.0 34-35 37.995723911064616 40.0 37.0 40.0 37.0 40.0 36-37 37.887772501238416 40.0 37.0 40.0 33.0 40.0 38-39 37.77118775412349 40.0 37.0 40.0 33.0 40.0 40-41 37.64796011033107 40.0 37.0 40.0 33.0 40.0 42-43 37.50323769908442 40.0 37.0 40.0 33.0 40.0 44-45 37.30438353687429 40.0 37.0 40.0 33.0 40.0 46-47 37.102620282091706 37.0 37.0 40.0 33.0 40.0 48-49 36.87623706729889 37.0 37.0 40.0 33.0 40.0 50-51 36.649219712569874 37.0 37.0 40.0 33.0 40.0 52-53 36.38596661169487 37.0 37.0 40.0 33.0 40.0 54-55 36.12049405467744 37.0 37.0 40.0 33.0 40.0 56-57 35.81742339667602 37.0 35.0 40.0 33.0 40.0 58-59 35.544398742240915 37.0 33.0 37.0 33.0 40.0 60-61 35.239532957652344 37.0 33.0 37.0 33.0 40.0 62-63 34.952377752323386 37.0 33.0 37.0 27.0 40.0 64-65 34.68281537278441 37.0 33.0 37.0 27.0 38.5 66-67 34.42007300782386 37.0 33.0 37.0 27.0 37.0 68-69 34.17172898956558 37.0 33.0 37.0 27.0 37.0 70-71 33.93868337023682 37.0 33.0 37.0 27.0 37.0 72-73 33.753420400268965 37.0 33.0 37.0 27.0 37.0 74-75 33.51914454139177 37.0 33.0 37.0 27.0 37.0 76-77 30.674005253937157 33.0 30.0 33.0 24.5 37.0 78-79 33.29165328023967 35.0 33.0 37.0 27.0 37.0 80-81 33.81281106625649 37.0 33.0 37.0 27.0 37.0 82-83 33.79364701700628 37.0 33.0 37.0 27.0 37.0 84-85 33.676942666538594 37.0 33.0 37.0 27.0 37.0 86-87 33.589395097634814 37.0 33.0 37.0 27.0 37.0 88-89 33.43661086208321 37.0 33.0 37.0 27.0 37.0 90-91 33.330226673242834 37.0 33.0 37.0 27.0 37.0 92-93 33.21992685360307 37.0 33.0 37.0 27.0 37.0 94-95 33.09567323099377 33.0 33.0 37.0 27.0 37.0 96-97 32.984241013805914 33.0 33.0 37.0 27.0 37.0 98-99 32.824153338763246 33.0 33.0 37.0 27.0 37.0 100-101 30.647892774585216 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0929501105907633 2306 2 0.08870320429809198 2306 3 0.09956165138471817 2306 4 0.0743770969622588 2306 5 0.07097396275828771 2306 6 0.07601299230888969 2306 7 0.12605748138329886 2306 8 0.07125118056452351 2306 9 0.10458365068468112 2306 10-11 0.07513684681825339 2306 12-13 0.07875104272612532 2306 14-15 0.14764275686083295 2306 16-17 0.17957281856262597 2306 18-19 0.10666693743191047 2306 20-21 0.12847898040182315 2306 22-23 0.06697363725951533 2306 24-25 0.08633843829652932 2306 26-27 0.11657698307308095 2306 28-29 0.16613178161752984 2306 30-31 0.0808142673202994 2306 32-33 0.09522305120828634 2306 34-35 0.08280977382604249 2306 36-37 0.052619393125738156 2306 38-39 -0.048817099467278524 2306 40-41 -0.008324831964515056 2306 42-43 -0.039134581572220384 2306 44-45 -0.01652318071322867 2306 46-47 -0.05834281627624449 2306 48-49 -0.02368441025728174 2306 50-51 -0.12141154489985695 2306 52-53 -0.03002130215801202 2306 54-55 -0.1325111534753063 2306 56-57 -0.28075495109747806 2306 58-59 -0.1475103637383981 2306 60-61 -0.2357813267102813 2306 62-63 -0.14841999650568738 2306 64-65 -0.4578020582569522 2306 66-67 -0.5414648154383173 2306 68-69 -0.4833792269432067 2306 70-71 -0.6207719170456087 2306 72-73 -0.48962671948289227 2306 74-75 -0.5729668481024248 2306 76-77 -0.866412558240885 2306 78-79 -0.5624051400341656 2306 80-81 -0.5683407097729685 2306 82-83 -0.48783885533941884 2306 84-85 -0.4910223681986423 2306 86-87 -0.5010564316693618 2306 88-89 -0.6356654222864222 2306 90-91 -0.7762907684575282 2306 92-93 -0.6955862191958957 2306 94-95 -0.7736326480011897 2306 96-97 -0.8021747465417164 2306 98-99 -0.708045682962684 2306 100-101 -0.9042764504543115 2307 1 0.07453813862303349 2307 2 0.08864750416351086 2307 3 0.06608599906499535 2307 4 0.08691481799414191 2307 5 0.10110469021883972 2307 6 0.08079973266847418 2307 7 0.15213013423792887 2307 8 0.08466397706619944 2307 9 0.08917926903342988 2307 10-11 0.09823548049925535 2307 12-13 0.10182732644688741 2307 14-15 0.187799829954713 2307 16-17 0.14710526475480634 2307 18-19 0.1315385219849503 2307 20-21 0.17507843455496896 2307 22-23 0.1770611485830571 2307 24-25 0.06925856493832327 2307 26-27 0.149508008283469 2307 28-29 0.18595661417582932 2307 30-31 0.15599499766429403 2307 32-33 0.054611630702432024 2307 34-35 0.05092780665381724 2307 36-37 0.10686219218187887 2307 38-39 0.04172346527664672 2307 40-41 0.019924264276291126 2307 42-43 -0.009337118816198142 2307 44-45 0.02770612102396086 2307 46-47 0.06327158961058643 2307 48-49 0.037546240665399466 2307 50-51 -0.03815620742361148 2307 52-53 -0.0742187924651887 2307 54-55 -0.0810679892634738 2307 56-57 -0.10604594271840284 2307 58-59 -0.04290220450274518 2307 60-61 -0.1326254528647155 2307 62-63 -0.1485755663708943 2307 64-65 -0.2864753987671804 2307 66-67 -0.2794843388176034 2307 68-69 -0.2877113474969022 2307 70-71 -0.3011265342183549 2307 72-73 -0.2822232587962432 2307 74-75 -0.3915160865111602 2307 76-77 -0.9184873348198259 2307 78-79 -0.3884250746237896 2307 80-81 -0.2929756924964053 2307 82-83 -0.10597747881633524 2307 84-85 -0.061504351860747875 2307 86-87 -0.19919734245157628 2307 88-89 -0.29491980881063284 2307 90-91 -0.32697730435535277 2307 92-93 -0.27375000431669605 2307 94-95 -0.32831085545933547 2307 96-97 -0.4046856293813761 2307 98-99 -0.3742950968035288 2307 100-101 -0.6269435840635964 2201 1 0.07887411250353438 2201 2 0.057341674682135135 2201 3 0.05579590777038845 2201 4 0.03221710755838103 2201 5 0.02930208111984456 2201 6 0.058234603469109913 2201 7 0.09379426572262872 2201 8 0.064367665689673 2201 9 0.08591297094514516 2201 10-11 0.05704996139716911 2201 12-13 0.0685391518228684 2201 14-15 0.14094837225102452 2201 16-17 0.10175183733289117 2201 18-19 0.12652200112918877 2201 20-21 0.12463393136935963 2201 22-23 0.12336091457009246 2201 24-25 0.09322662675625537 2201 26-27 0.10678024163718192 2201 28-29 0.09165796270819015 2201 30-31 0.14623989797140524 2201 32-33 0.1326976138532956 2201 34-35 0.12723164238124696 2201 36-37 0.05441924703323053 2201 38-39 0.09739254523672258 2201 40-41 0.015799985294918883 2201 42-43 0.04419585659055514 2201 44-45 0.025192050523877185 2201 46-47 0.08247662187089588 2201 48-49 0.0300525886111771 2201 50-51 0.0383544584202653 2201 52-53 -0.031837543597085016 2201 54-55 0.01779742506364812 2201 56-57 -0.02373981823753013 2201 58-59 0.06296186063493536 2201 60-61 0.024249839584228994 2201 62-63 -0.054015576933544196 2201 64-65 -0.046129754036876136 2201 66-67 -0.052332899247325315 2201 68-69 -0.051926140640084384 2201 70-71 -0.16576814479495994 2201 72-73 -0.2014565383057132 2201 74-75 -0.3680213957144005 2201 76-77 -0.8648300000436571 2201 78-79 -0.1803042344981023 2201 80-81 -0.07234276966849507 2201 82-83 0.1481572233269688 2201 84-85 0.026961986206373467 2201 86-87 -0.06272397634896976 2201 88-89 0.012174024201208056 2201 90-91 -0.09104948174485372 2201 92-93 -0.13854553953751036 2201 94-95 -0.1920688009348055 2201 96-97 -0.17565790538455417 2201 98-99 -0.20359489894982374 2201 100-101 -0.38348595015324705 1101 1 0.07607868316886623 1101 2 0.0691772937484103 1101 3 0.059546810820990004 1101 4 0.03545758343854999 1101 5 0.06694534681816577 1101 6 0.05907027391006636 1101 7 0.06986564003665308 1101 8 0.0715284966287939 1101 9 0.039591297609497644 1101 10-11 0.0806633066094804 1101 12-13 0.08513944452271716 1101 14-15 0.12759198530686433 1101 16-17 0.13569318771013883 1101 18-19 0.1095344372922824 1101 20-21 0.0852756654850424 1101 22-23 0.12300406797120189 1101 24-25 0.11996626310646263 1101 26-27 0.05822229680978097 1101 28-29 0.032743795196076064 1101 30-31 0.14990288751040026 1101 32-33 0.05247929970614962 1101 34-35 0.0653883024228179 1101 36-37 -0.016477201609760073 1101 38-39 0.010283512027932318 1101 40-41 0.05453014329062 1101 42-43 0.06444747211149604 1101 44-45 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0.0637162945651184 2304 5 0.05743329668054997 2304 6 0.07448642605369571 2304 7 0.08354766192300644 2304 8 0.059673370829706585 2304 9 0.03831824226709557 2304 10-11 0.06751489780690889 2304 12-13 0.0408987456144132 2304 14-15 0.06572114839302401 2304 16-17 0.08040412435442335 2304 18-19 0.10899348956080246 2304 20-21 0.1135142188043261 2304 22-23 0.12627790543653106 2304 24-25 0.1084244814405011 2304 26-27 0.12591695376939782 2304 28-29 0.1010625857691565 2304 30-31 0.12310171557938787 2304 32-33 0.09444569899647348 2304 34-35 0.08693883457296891 2304 36-37 0.08756728080321352 2304 38-39 0.0579659569488129 2304 40-41 0.09321704695882005 2304 42-43 0.080480772489814 2304 44-45 0.026852088413690467 2304 46-47 0.04764783744821699 2304 48-49 0.06400174563636796 2304 50-51 -0.02247910628640426 2304 52-53 -0.020721163085205774 2304 54-55 -0.0532915515623813 2304 56-57 -0.031096846140322043 2304 58-59 0.048422855385112484 2304 60-61 -0.06022669624982058 2304 62-63 0.017554822491653965 2304 64-65 -0.08797410711425613 2304 66-67 -0.0897352132855147 2304 68-69 -0.12612955133246828 2304 70-71 -0.18260531767878518 2304 72-73 -0.12907467309856457 2304 74-75 -0.2359020242518568 2304 76-77 -0.8708340965986636 2304 78-79 -0.19520545678664547 2304 80-81 -0.057371948340254164 2304 82-83 -0.0436263630761502 2304 84-85 -0.005073917670891603 2304 86-87 -0.019696827527980076 2304 88-89 -0.0697628151724956 2304 90-91 -0.09409232169601722 2304 92-93 0.03611329909025329 2304 94-95 -0.032007714909916274 2304 96-97 -0.11708453260414586 2304 98-99 -0.04551916934794775 2304 100-101 -0.07693415688518712 2202 1 0.07538959243048993 2202 2 0.04629821550025781 2202 3 0.025407034284896213 2202 4 0.01583676177358484 2202 5 0.03653145881965969 2202 6 0.03368062271007943 2202 7 0.05323868210884086 2202 8 0.0656424685355077 2202 9 0.054216677609950636 2202 10-11 0.04597063348565911 2202 12-13 0.03052599882487783 2202 14-15 0.039168202012874076 2202 16-17 0.0299952999593458 2202 18-19 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0.12033392624763906 1213 60-61 0.046684783388158735 1213 62-63 0.14632395874837556 1213 64-65 0.2223564477364448 1213 66-67 0.20236677059537556 1213 68-69 0.24822926494667286 1213 70-71 0.2720858535065034 1213 72-73 0.3336126694216972 1213 74-75 0.3910365118719028 1213 76-77 0.8292333044769329 1213 78-79 0.2595217296441845 1213 80-81 0.21119830057736522 1213 82-83 0.05635167584570411 1213 84-85 0.17571820657280313 1213 86-87 0.2611946885256984 1213 88-89 0.3244677318479319 1213 90-91 0.2593771443548505 1213 92-93 0.343085948470673 1213 94-95 0.2938051786834066 1213 96-97 0.4389409522761625 1213 98-99 0.4903547067874072 1213 100-101 0.7760122547805963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 30.0 8 364.0 9 1110.0 10 749.0 11 261.0 12 218.0 13 472.0 14 1124.0 15 2677.0 16 4776.0 17 7594.0 18 10612.0 19 14224.0 20 18958.0 21 24886.0 22 31982.0 23 41468.0 24 53908.0 25 68781.0 26 88319.0 27 112288.0 28 143545.0 29 183674.0 30 238094.0 31 315336.0 32 431264.0 33 630431.0 34 1018510.0 35 1921879.0 36 4021612.0 37 4840653.0 38 635975.0 39 27.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.987140008841916 20.855593644760532 31.129593380931293 28.02767296546626 2 27.73568789148572 21.45642442211669 31.576691042072124 19.23119664432546 3 21.118043800389135 19.623674575556088 39.326450153654655 19.931831470400123 4 21.12688097171475 19.66108565598332 38.1623491229172 21.049684249384725 5 30.10849598178477 18.915552767949855 31.75357350332347 19.222377746941905 6 29.941340542361516 19.553903710825193 31.882944239006516 18.621811507806775 7 30.184616990779645 26.19939695155405 24.40038224639582 19.215603811270483 8 26.641290306262615 21.21924922459626 31.90247227798779 20.236988191153333 9 22.714432398005517 20.268907083666647 35.859130121720966 21.157530396606873 10-11 28.793722754238914 21.24275936757057 28.094593922350615 21.8689239558399 12-13 27.134600321650954 25.910075230374353 24.131079624082222 22.824244823892474 14-15 26.862099083238284 23.423411878698253 25.73974808224298 23.974740955820486 16-17 26.376407222912395 23.031303924093173 25.068203509208114 25.524085343786318 18-19 26.1413408921576 23.956306029596515 25.747322512795133 24.15503056545075 20-21 24.917450365445053 24.428566825329245 26.10213558435656 24.55184722486914 22-23 25.06631999184168 24.456120906746786 25.49548615063134 24.982072950780193 24-25 25.452464595927676 24.256602602859555 25.337418682501127 24.95351411871164 26-27 25.247900194138705 24.330527537963334 25.457187908239526 24.964384359658435 28-29 25.185139865908283 24.903797969294526 24.85825359087035 25.05280857392684 30-31 25.441945250640817 24.738157344537147 24.813483188092412 25.006414216729624 32-33 25.24148710692002 25.11734313192882 24.756747095548953 24.88442266560221 34-35 25.061728918260997 25.21879072265449 24.717556480631657 25.001923878452857 36-37 25.155321284782605 25.110607912834016 24.830643142692836 24.903427659690543 38-39 25.16721173453068 24.5660943863313 25.05089725478051 25.215796624357505 40-41 25.075021593303248 24.967633589224068 24.596578571370912 25.360766246101768 42-43 25.119408274184163 24.861130955311936 24.710382297519864 25.309078472984037 44-45 25.543209568849946 24.606966731355875 24.673465474485567 25.176358225308615 46-47 24.9849675623609 24.729446395921777 24.983702805915993 25.301883235801327 48-49 25.24727766603936 24.4807016813324 24.8247607931304 25.447259859497844 50-51 25.047186425700733 24.690467945116428 25.438676683952323 24.823668945230516 52-53 25.10241952871443 24.55080815212291 25.47720379668196 24.869568522480698 54-55 24.905734237620898 24.674350052284282 24.91484295071715 25.50507275937767 56-57 25.051627678430233 24.874134564223173 25.16357721404604 24.91066054330056 58-59 24.94149521950243 25.18496741683043 25.122644401276524 24.75089296239062 60-61 25.434258776130335 25.043021895622086 24.72066714009813 24.80205218814945 62-63 24.983693511201427 25.267846187295362 25.209966224579638 24.538494076923573 64-65 25.256254053428563 25.166126658037292 24.943833944655395 24.63378534387875 66-67 25.62253117021144 25.01846544218653 25.053138822376813 24.305864565225217 68-69 25.248561668767 24.788706097340256 25.539306088862663 24.423426145030078 70-71 25.230579846598665 25.19967204358606 25.040507622652058 24.52924048716322 72-73 24.987117637225463 24.760663243952035 25.48389222731536 24.768326891507144 74-75 24.831669930766633 24.75420011923401 25.46591721881863 24.948212731180732 76-77 24.797249085060503 24.735416482180447 25.172319100103813 25.29501533265523 78-79 25.12114701175413 24.76275460952641 24.967723729113427 25.14837464960603 80-81 24.868941626718428 24.611942331120503 25.424007635781713 25.095108406379353 82-83 24.62034505964238 25.44856569568246 25.22164252458225 24.709446720092906 84-85 24.942809881771602 25.04138540574315 25.046589170820234 24.96921554166502 86-87 24.617836282509202 25.265031586755715 25.38289703372239 24.734235097012693 88-89 24.80961206846383 26.257525785416863 24.45317397092291 24.47968817519639 90-91 24.859860409204543 25.266994903351303 24.725623353635005 25.14752133380915 92-93 24.72305988345479 25.563812012054786 24.755601198696503 24.95752690579392 94-95 24.804613757227497 25.792824257663817 24.247981679700153 25.15458030540853 96-97 24.654732528091046 24.995917766492475 24.82027912317863 25.529070582237846 98-99 24.9186293622327 25.15294746382412 24.780340236261893 25.14808293768128 100-101 25.198416394906847 25.78590969203794 24.03711904349183 24.978554869563386 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1709.0 1 1379.5 2 1330.5 3 1588.5 4 1725.0 5 1798.5 6 1736.0 7 1817.5 8 1941.5 9 2110.0 10 2292.0 11 2449.5 12 2599.5 13 2810.0 14 3077.0 15 3331.0 16 3644.0 17 4002.0 18 4377.5 19 4872.5 20 5454.0 21 6156.0 22 6948.0 23 7919.5 24 9120.5 25 10566.0 26 12225.5 27 13986.0 28 16005.5 29 18574.0 30 21661.0 31 25390.0 32 30375.0 33 36852.0 34 44453.0 35 54102.0 36 66088.0 37 80256.5 38 97085.5 39 123255.0 40 167718.5 41 227615.0 42 286255.0 43 349179.5 44 444501.0 45 567641.5 46 713203.5 47 877802.0 48 1010103.5 49 1046547.0 50 1000844.5 51 946233.5 52 912396.5 53 909332.5 54 877553.5 55 798165.0 56 725156.5 57 606105.0 58 464053.5 59 362153.5 60 276982.5 61 197338.5 62 131662.0 63 83361.5 64 47927.5 65 26838.5 66 16926.5 67 12055.0 68 9458.5 69 7476.0 70 5967.5 71 4858.0 72 3884.5 73 2998.5 74 2307.0 75 1745.5 76 1429.0 77 1224.5 78 925.0 79 583.5 80 356.0 81 223.0 82 155.5 83 113.5 84 69.0 85 37.5 86 33.5 87 28.5 88 20.0 89 20.0 90 12.5 91 3.5 92 1.0 93 2.5 94 2.0 95 2.5 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15384300775121212 2 0.0 3 0.0 4 0.06895691615468628 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0020415982882594176 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0033970581073179762 22-23 0.0 24-25 0.0 26-27 9.68666074165918E-4 28-29 5.616917860614872E-4 30-31 0.002610016922058168 32-33 0.0 34-35 0.0 36-37 3.363423868631659E-5 38-39 4.776061893456957E-4 40-41 5.650552099301188E-4 42-43 0.0 44-45 7.365898272303334E-4 46-47 0.008623818799171575 48-49 0.003232250337755025 50-51 0.0020180543211789958 52-53 0.0048366035230923265 54-55 0.006161792527333201 56-57 0.016215066470673233 58-59 0.01961548800185984 60-61 0.008610365103697048 62-63 0.0037401273419184053 64-65 0.012404307227513562 66-67 0.028649644513004478 68-69 0.02081286689909271 70-71 0.015091682898550257 72-73 0.023069724314944553 74-75 0.017456169878198313 76-77 0.03169690653798476 78-79 0.002650378008481748 80-81 3.0270814817684936E-5 82-83 0.0013453695474526638 84-85 0.010537606980422989 86-87 0.012249589729556505 88-89 0.013944755359346861 90-91 0.009609301992680652 92-93 0.021253475425883458 94-95 0.02276028931903044 96-97 0.017604160528418105 98-99 0.020870045104859448 100-101 0.34476440023021965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4865804E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.202059665213927 #Duplication Level Percentage of deduplicated Percentage of total 1 80.07482650224227 20.180505551911633 2 8.463010170724205 4.2657057453980745 3 3.1764745566178485 2.4016110390275087 4 1.6542498032766835 1.6676200897338949 5 0.9905227094401361 1.2481606211529832 6 0.6096100391742221 0.9218057147869286 7 0.46974500640286204 0.828697917516086 8 0.5037983150645773 1.0157404156393375 9 0.37586627967056163 0.8525343965759531 >10 2.704831376317753 14.318833384078228 >50 0.4032027173006285 7.259525732978038 >100 0.48475146845922856 26.12084212204372 >500 0.07313999149479682 12.513906559272344 >1k 0.015751143170115615 6.042926710864256 >5k 2.1992064402106962E-4 0.36158399902108695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2108325927073974E-4 0.0 0.0 2 1.2108325927073974E-4 0.0 0.0 3 1.2108325927073974E-4 0.0 0.0 4 1.2108325927073974E-4 0.0 0.0 5 1.2108325927073974E-4 0.0 0.0 6 1.2108325927073974E-4 0.0 0.0 7 1.345369547452664E-4 0.0 0.0 8 1.412638024825297E-4 0.0 0.0 9 1.4799065021979302E-4 0.0 0.0 10-11 1.6817119343158298E-4 0.0 0.0 12-13 2.1525912759242621E-4 0.0 0.0 14-15 2.825276049650594E-4 0.0 0.0 16-17 4.069742881044308E-4 0.0 0.0 18-19 5.415112428496972E-4 0.0 0.0 20-21 7.130458601499118E-4 0.0 0.0 22-23 9.18214716136443E-4 3.3634238686316596E-6 0.0 24-25 0.0011603812346779225 6.726847737263319E-6 0.0 26-27 0.0014260917202998237 6.726847737263319E-6 0.0 28-29 0.0017220730207394097 6.726847737263319E-6 0.0 30-31 0.0021290473088438405 6.726847737263319E-6 0.0 32-33 0.002468753119575638 6.726847737263319E-6 0.0 34-35 0.0028992713747604906 1.3453695474526638E-5 0.0 36-37 0.003417238650529766 1.3453695474526638E-5 0.0 38-39 0.0040226549468834645 1.3453695474526638E-5 0.0 40-41 0.004745791078639271 1.3453695474526638E-5 0.0 42-43 0.005633734979958029 1.3453695474526638E-5 0.0 44-45 0.006632671868941633 1.3453695474526638E-5 0.0 46-47 0.007776235984276398 1.3453695474526638E-5 0.0 48-49 0.009209054552313484 1.3453695474526638E-5 0.0 50-51 0.010947944692396051 1.3453695474526638E-5 0.0 52-53 0.013100535968320315 1.3453695474526638E-5 0.0 54-55 0.015754277400670695 1.3453695474526638E-5 0.0 56-57 0.01905379621579835 1.3453695474526638E-5 0.0 58-59 0.023399339854070455 1.3453695474526638E-5 0.0 60-61 0.029191155755854174 1.3453695474526638E-5 0.0 62-63 0.03581373735318991 1.3453695474526638E-5 0.0 64-65 0.044215570177031795 1.3453695474526638E-5 0.0 66-67 0.054951619165704055 1.3453695474526638E-5 0.0 68-69 0.06895691615468628 1.3453695474526638E-5 0.0 70-71 0.08616082924273723 1.3453695474526638E-5 0.0 72-73 0.10775737390322111 1.3453695474526638E-5 0.0 74-75 0.1347286699057784 1.3453695474526638E-5 0.0 76-77 0.16838981598304403 1.3453695474526638E-5 0.0 78-79 0.2112835605797036 1.68171193431583E-5 0.0 80-81 0.2646947316135744 2.018054321178996E-5 0.0 82-83 0.3295348169530555 2.018054321178996E-5 0.0 84-85 0.40948676573429865 2.018054321178996E-5 0.0 86-87 0.5058858572331507 2.018054321178996E-5 0.0 88-89 0.6230978156310953 2.018054321178996E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 162605 0.0 73.09115 1 TTTGTGT 175490 0.0 67.781715 2 TAACTTT 17550 0.0 66.94793 1 AACTTTG 17980 0.0 65.483055 2 TTGTGTT 199155 0.0 61.280544 3 TGTGTTT 212215 0.0 57.83166 4 GTGTTTG 237700 0.0 51.661304 5 ACTTTGT 36295 0.0 46.65007 1 TTTGAGG 70325 0.0 38.02641 8 TTGAGGG 33595 0.0 37.3838 9 GTTTGAG 182825 0.0 36.92886 7 TGTTTGA 338360 0.0 36.243267 6 TTGAGTC 21135 0.0 36.171883 4 TTTGAGC 43535 0.0 34.672436 8 TTGAGGC 12990 0.0 33.2998 9 TTGAGGT 20105 0.0 33.11131 9 TTTGAGT 69125 0.0 32.264526 3 GTTTGAC 65065 0.0 31.65005 7 TTTGAGA 22540 0.0 31.324883 8 GTTTGAA 25805 0.0 29.994015 2 >>END_MODULE