FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l08n01_30429.34100000035b60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l08n01_30429.34100000035b60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14865804
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1626050.073.091151
TTTGTGT1754900.067.7817152
TAACTTT175500.066.947931
AACTTTG179800.065.4830552
TTGTGTT1991550.061.2805443
TGTGTTT2122150.057.831664
GTGTTTG2377000.051.6613045
ACTTTGT362950.046.650071
TTTGAGG703250.038.026418
TTGAGGG335950.037.38389
GTTTGAG1828250.036.928867
TGTTTGA3383600.036.2432676
TTGAGTC211350.036.1718834
TTTGAGC435350.034.6724368
TTGAGGC129900.033.29989
TTGAGGT201050.033.111319
TTTGAGT691250.032.2645263
GTTTGAC650650.031.650057
TTTGAGA225400.031.3248838
GTTTGAA258050.029.9940152