FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_envcontrol.34200000035d42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_envcontrol.34200000035d42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14045726
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG716140.5098632851018167Illumina Single End PCR Primer 1 (100% over 50bp)
CGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT351260.25008319256690614No Hit
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG298540.2125486429110179No Hit
CTTTGTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAA266000.18938145311961804No Hit
GTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGAC201700.14360240260987575No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA187030.13315794427429384No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG181700.12936319560840073No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC170240.12120412999655553No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT164450.1170818795696285No Hit
TGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGACG151010.1075131324646373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1306250.041.5947721
TTTGTGT1445650.037.639682
GTATCAG80150.036.916893
TTGTGTT1539500.035.910373
GAGCGTA33500.034.6171494-95
ATCAGCT87300.034.0011565
TGTGTTT1632200.033.9814574
GCAAGAG111700.033.3548857
ATCGGAG51600.032.65797894-95
CGGGCAA114750.032.3836254
CGGAAGG63850.032.06812794-95
GGGCAAG116950.031.776145
CGGTATC92450.031.7007941
CAAGAGC119800.031.1021128
CCGGGCA119800.030.9024773
CGTATCA183350.030.70334648-49
GGTATCA96650.030.3722212
GCCGTAT211200.029.9984446-47
CCGTATC215500.029.76933148-49
CGTGTAA29000.029.36674994-95
Produced by FastQC (version 0.11.2)