FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_8561.34200000035d0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_8561.34200000035d0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16162391
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1935100.072.081521
TTTGTGT2098850.066.523212
ACATCCG20400.064.2767594-95
GTTCTCG18900.063.11478494-95
TTGTGTT2385650.059.9287953
TGTGTTT2497450.057.46124
TAACTTT178000.056.0631261
AACTTTG181600.055.3642432
ACTTTGT425100.052.6236531
GTGTTTG2807350.051.1335035
TTTGAGG820050.037.2541478
GTTTGAG2158850.036.24097
TCTCGCG15550.035.72078794-95
TTGAGGG418050.035.6611759
TGTTTGA4049400.035.4179426
TTGAGGC141900.034.1637659
TTTGAGC533400.034.0317088
TCCTAGG11000.033.11226794-95
AGTTCTG32200.031.95539394-95
GTTTGAC771500.031.3012927
Produced by FastQC (version 0.11.2)