FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_7153.34200000035cc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_7153.34200000035cc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15494087
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282790.18251478773805777No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167320.1079895833810666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1385700.066.402781
TTTGTGT1471900.062.56542
AGATCGA9600.058.616294-95
ACTTTGT375050.054.9102331
TTGTGTT1734600.054.5330963
TGTGTTT1807200.052.5113754
AACTTTG172700.046.7955782
TAACTTT176500.045.6022641
GTGTTTG2116550.044.8986665
TTGAGTC184650.035.641234
GCGTCGG9950.032.83799494-95
TTTGAGG576850.032.5809338
GTTTGAA209450.032.4255522
TTTGAGT590400.032.317673
GTTTGAG1556450.031.872817
TGTTTGA3015300.031.470691
GTTTGAC605600.030.174367
TTGAGGG321550.029.71899
TTTGACC162050.028.9657678
GTTCTCG13700.028.48689594-95
Produced by FastQC (version 0.11.2)