FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_7098.34200000035c86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_7098.34200000035c86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20351526
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG2877150.065.619791
TTTGTGT3091400.061.1057132
TAACTTT397350.060.2515681
AACTTTG424450.057.219992
TTGTGTT3454550.056.1099133
TGTGTTT3700450.052.6866044
ACTTTGT830550.048.578891
GTGTTTG4052350.048.133795
AGATCGA18850.046.64456694-95
GACCTAG16700.043.9652294-95
GTTCTCG23350.040.37264394-95
TTTGAGG1032200.038.8283468
GTTTGAG2700550.038.2093127
TTGAGGG526350.037.4638069
TGTTTGA5212850.037.3823556
GTTTGAC1082550.034.6527337
TTTGAGA340750.033.9287768
TTTGAGC674550.033.0377858
TTTGATG380750.032.2061128
GTTTGAA352850.031.8481887
Produced by FastQC (version 0.11.2)