FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_7047.34200000035c43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_7047.34200000035c43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18850123
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1673300.067.899951
TTTGTGT1801200.063.102352
TTGTGTT2019700.057.514863
AACTTTG192500.057.1817782
TAACTTT192250.056.988251
TGTGTTT2163550.053.8858384
ACTTTGT439300.052.5924531
GTTCTCG19600.051.1088294-95
GTGTTTG2429550.048.0284425
TCTCGCG12350.043.5055294-95
TTGAGTC223750.035.8256384
TTTGAGG753400.034.3016368
GTTTGAG1948650.033.202117
TTGAGGG398300.032.9696169
TTTGAGT712750.032.619263
TGTTTGA3588350.032.4697046
GCGTCGG10350.031.67535294-95
TTTGAGC480400.030.925978
TTGAGGC137850.029.4025349
TGAGTCT54000.028.2185085
Produced by FastQC (version 0.11.2)