FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_619.34200000035c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_619.34200000035c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16986345
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA444020.2613981995538181No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT376920.22189588166259427No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244770.14409809761899928No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT181780.10701537028713357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1880450.059.5403821
TTTGTGT2056750.054.5076452
ACTTTGT628350.052.8664361
TTGTGTT2219550.051.6695143
AGATCGA11100.049.98052694-95
TGTGTTT2368950.048.578764
AACTTTG325200.047.6178862
TAACTTT324000.047.4043161
ACATCCG18450.043.87201394-95
GTTCTCG10700.043.34912594-95
GACCTAG8700.041.8152594-95
GTGTTTG2808850.041.033885
TCTCGCG10800.036.21084694-95
GCGTCGG13200.034.45006694-95
TTTGAGG924650.032.7513548
TTGAGGG528050.032.5170829
GTTTGAG2199000.031.552617
TGTTTGA3750100.030.7018096
TTGAGTC247950.028.9738624
CCTAGCG9100.027.9840594-95
Produced by FastQC (version 0.11.2)