FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_30515.34200000035be4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_30515.34200000035be4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17987162
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1803800.070.415921
TTTGTGT1980900.064.189612
TAACTTT190000.059.167491
TTGTGTT2202800.058.9346433
AACTTTG199200.056.4120142
TGTGTTT2344300.055.581544
GTTCTCG20600.052.0062794-95
ACTTTGT437950.051.3063621
GTGTTTG2582750.050.4583855
TTTGAGG768200.037.15438
ACATCCG29400.036.43976694-95
GTTTGAG2088350.035.53187
TGTTTGA3696650.035.2380686
TTGAGGG394900.035.0430839
TTTGAGC567050.032.987168
TTGAGTC267550.032.4441074
AGATCGA17000.031.5096894-95
TTGAGGT233700.031.4278899
GTTTGAC665850.030.8001447
TTTGAGT792150.030.7564033
Produced by FastQC (version 0.11.2)