FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_30455.34200000035ba1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_30455.34200000035ba1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939697
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1536800.073.625141
TTTGTGT1677050.067.50722
TTGTGTT1856550.062.099913
TAACTTT151200.060.8072051
TGTGTTT1970750.058.7181134
AACTTTG160050.058.0875632
GTGTTTG2158000.053.6569675
GTTCTCG16950.049.78175794-95
ACTTTGT319500.047.6777761
TTTGAGG645200.038.764318
GTTTGAG1767700.037.082787
TGTTTGA3151800.036.7076726
TTGAGGG328000.036.344529
TTTGAGC482200.035.3089948
TTGAGGC129300.034.239249
TTGAGTC224750.033.046834
GTTTGAC559950.032.6246037
TCTCGCG11900.032.022894-95
ACATCCG25500.032.022894-95
CTAGTTA13950.031.86976294-95
Produced by FastQC (version 0.11.2)