FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n02_30429.34200000035b6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n02_30429.34200000035b6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14661082
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1722750.072.762121
TTTGTGT1861850.067.3641362
TAACTTT172500.064.703881
GTTCTCG15600.063.0020794-95
AACTTTG180650.062.225042
TTGTGTT2091150.061.4279443
TGTGTTT2216900.058.158014
GTGTTTG2510850.051.3722725
ACTTTGT370850.047.313061
TCTCGCG14950.041.39266694-95
TTTGAGG720350.038.8477338
CTAGTTA9950.038.41332294-95
TTGAGGG343950.037.934739
GTTTGAG1899200.037.210337
AGATCGA13600.036.8026894-95
ACATCCG21250.036.40119694-95
TGTTTGA3554300.036.2662166
TTGAGTC228900.035.4488644
TTTGAGC454750.035.0964628
TTGAGGC132400.034.4615149
Produced by FastQC (version 0.11.2)