FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_envcontrol.34100000035d45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_envcontrol.34100000035d45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14045726
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCAGAACTCCAGTCACTGGTGAATCTCGTATGC1889061.344935818910322TruSeq Adapter, Index 4 (97% over 35bp)
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG331920.23631387939647974No Hit
CGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT296820.21132407110889107No Hit
CTTTGTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAA249370.17754155249789155No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG231490.16481170143857285No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT211280.15042298276358232No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA178350.12697812843565365No Hit
TGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGACG166280.11838476701026349No Hit
GTGTTTGAGAGGTGTTTTATGTTGATAAAGGAAAAAGGTGTTTAAAGGAC163630.11649807208256803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1241150.040.1326331
GTATCAG84100.040.05953
ATCAGCT92950.036.575565
TTTGTGT1378400.036.162252
CGTATGC310650.035.12013644-45
TTGTGTT1455000.035.0389823
CTCGTAT310350.034.4869942-43
TATGCCG319500.034.1926546-47
TCGTATG312800.034.03466842-43
GTATGCC314250.033.73536744-45
CGGTATC102750.033.3496931
TGTGTTT1540050.033.2366144
CCGTCTT331550.032.8276450-51
GCCGTCT321650.032.7961648-49
GCTTGAA297400.032.45200358-59
GGTATCA106200.031.8574312
TCTTCTG330050.031.74045852-53
ATGCCGT332600.031.51780146-47
GCAAGAG97050.031.3611077
GCTCACT108550.031.2755629