##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_8561.34100000035d01.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16162391 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74111992464481 33.0 33.0 33.0 33.0 33.0 2 32.76784344593569 33.0 33.0 33.0 33.0 33.0 3 32.796286638530155 33.0 33.0 33.0 33.0 33.0 4 36.71427915585014 37.0 37.0 37.0 37.0 37.0 5 36.72491285478739 37.0 37.0 37.0 37.0 37.0 6 36.66387392805928 37.0 37.0 37.0 37.0 37.0 7 36.62362746947528 37.0 37.0 37.0 37.0 37.0 8 36.615746581059696 37.0 37.0 37.0 37.0 37.0 9 36.60565717040257 37.0 37.0 37.0 37.0 37.0 10-11 36.59709043049385 37.0 37.0 37.0 37.0 37.0 12-13 36.56174887737835 37.0 37.0 37.0 37.0 37.0 14-15 38.63760696050479 40.0 37.0 40.0 37.0 40.0 16-17 38.59191158659631 40.0 37.0 40.0 37.0 40.0 18-19 38.5434930388703 40.0 37.0 40.0 37.0 40.0 20-21 38.484332980188384 40.0 37.0 40.0 37.0 40.0 22-23 38.43423893779082 40.0 37.0 40.0 37.0 40.0 24-25 38.393585515905414 40.0 37.0 40.0 37.0 40.0 26-27 38.295018199968055 40.0 37.0 40.0 37.0 40.0 28-29 38.22562645588762 40.0 37.0 40.0 37.0 40.0 30-31 38.16854965951511 40.0 37.0 40.0 37.0 40.0 32-33 38.07621932299497 40.0 37.0 40.0 37.0 40.0 34-35 37.98290979967011 40.0 37.0 40.0 37.0 40.0 36-37 37.875168160453484 40.0 37.0 40.0 33.0 40.0 38-39 37.7406475007318 40.0 37.0 40.0 33.0 40.0 40-41 37.60478502221608 40.0 37.0 40.0 33.0 40.0 42-43 37.47367938939232 40.0 37.0 40.0 33.0 40.0 44-45 37.313597474532074 40.0 37.0 40.0 33.0 40.0 46-47 37.11354638060668 37.0 37.0 40.0 33.0 40.0 48-49 36.87219910717418 37.0 37.0 40.0 33.0 40.0 50-51 36.65748598087993 37.0 37.0 40.0 33.0 40.0 52-53 36.41568134937461 37.0 37.0 40.0 33.0 40.0 54-55 36.12078794529844 37.0 37.0 40.0 33.0 40.0 56-57 35.85446144694804 37.0 37.0 40.0 33.0 40.0 58-59 35.559336672402 37.0 33.0 37.0 33.0 40.0 60-61 35.25807895626334 37.0 33.0 37.0 33.0 40.0 62-63 34.98212615942778 37.0 33.0 37.0 27.0 40.0 64-65 34.68988081652028 37.0 33.0 37.0 27.0 40.0 66-67 34.43961728806091 37.0 33.0 37.0 27.0 37.0 68-69 34.17715658530969 37.0 33.0 37.0 27.0 37.0 70-71 33.93117036334537 37.0 33.0 37.0 27.0 37.0 72-73 33.72471328654281 37.0 33.0 37.0 27.0 37.0 74-75 33.462411625854116 37.0 33.0 37.0 27.0 37.0 76-77 30.678805041902528 33.0 30.0 33.0 24.5 37.0 78-79 33.28472164174224 35.0 33.0 37.0 27.0 37.0 80-81 33.791837420589566 37.0 33.0 37.0 27.0 37.0 82-83 33.75478346613444 37.0 33.0 37.0 27.0 37.0 84-85 33.675436233413734 37.0 33.0 37.0 27.0 37.0 86-87 33.50801765654599 37.0 33.0 37.0 27.0 37.0 88-89 33.34845692076129 37.0 33.0 37.0 27.0 37.0 90-91 33.24124809256254 37.0 33.0 37.0 27.0 37.0 92-93 33.13901693134388 37.0 33.0 37.0 27.0 37.0 94-95 33.03477684706428 35.0 33.0 37.0 27.0 37.0 96-97 32.905534707086346 33.0 33.0 37.0 27.0 37.0 98-99 32.71505373183955 33.0 33.0 37.0 27.0 37.0 100-101 30.687613670526844 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09649141312230114 2306 2 0.07760719517332149 2306 3 0.06667513196816799 2306 4 0.07047589904060203 2306 5 0.08184401984156864 2306 6 0.0654221334082834 2306 7 0.11261989645230841 2306 8 0.10135626862133051 2306 9 0.07280410091736655 2306 10-11 0.08928307744606911 2306 12-13 0.0812054413811154 2306 14-15 0.1364345043964974 2306 16-17 0.14186575326120732 2306 18-19 0.16913049100433142 2306 20-21 0.15884304412015382 2306 22-23 0.14822682609215576 2306 24-25 0.10844134716013798 2306 26-27 0.11949413789083252 2306 28-29 0.1158980523032298 2306 30-31 0.0894406762007165 2306 32-33 0.10228644650988628 2306 34-35 0.057142234081432264 2306 36-37 0.086204744540332 2306 38-39 0.06604948290480195 2306 40-41 0.02393482660252033 2306 42-43 0.061049116872482045 2306 44-45 -0.00690064044154326 2306 46-47 -0.002158247179934847 2306 48-49 -0.025111986971872113 2306 50-51 -0.052482574474332466 2306 52-53 -0.04703425005354234 2306 54-55 -0.01851844646166967 2306 56-57 -0.01209640898662201 2306 58-59 -0.08526663039773297 2306 60-61 -0.21459080436203948 2306 62-63 -0.28816386771851654 2306 64-65 -0.28227851782785507 2306 66-67 -0.34875193427072304 2306 68-69 -0.3471504109570702 2306 70-71 -0.349313945848408 2306 72-73 -0.36983904251579247 2306 74-75 -0.41298367664591495 2306 76-77 -0.25225565652367976 2306 78-79 -0.27837621665681667 2306 80-81 -0.20197723674365164 2306 82-83 -0.30778067013498145 2306 84-85 -0.3025002642199084 2306 86-87 -0.24381650087824625 2306 88-89 -0.39083891744254373 2306 90-91 -0.46047534059805884 2306 92-93 -0.45364789083652823 2306 94-95 -0.4297443411898456 2306 96-97 -0.49176420493207473 2306 98-99 -0.4598608749164512 2306 100-101 -0.7996625825959249 2307 1 0.10591759491275354 2307 2 0.09752900784813079 2307 3 0.06706790798664031 2307 4 0.04869081784320883 2307 5 0.07424144488545892 2307 6 0.08379566927210647 2307 7 0.08132536707327631 2307 8 0.1123145806117094 2307 9 0.0845743947201072 2307 10-11 0.09842279138845811 2307 12-13 0.10707865586200427 2307 14-15 0.15318973918602552 2307 16-17 0.11597244574955567 2307 18-19 0.15126604328199988 2307 20-21 0.1388022429755793 2307 22-23 0.1298072134799284 2307 24-25 0.10557233408737687 2307 26-27 0.1586121089334256 2307 28-29 0.12235160281736057 2307 30-31 0.08558799673598827 2307 32-33 0.08579135534865401 2307 34-35 0.06190492251710822 2307 36-37 0.07491128651720658 2307 38-39 0.0443619521629941 2307 40-41 0.055783332519006024 2307 42-43 0.030884087543228134 2307 44-45 0.015984099198846025 2307 46-47 0.11796997516283625 2307 48-49 0.04657708318007536 2307 50-51 0.05031036088823981 2307 52-53 -0.09082433596488926 2307 54-55 -0.04155018087213591 2307 56-57 -0.07872188556447668 2307 58-59 -0.05252457197830296 2307 60-61 -0.18759693607674421 2307 62-63 -0.2161010181620142 2307 64-65 -0.2980760188677323 2307 66-67 -0.31094834572012786 2307 68-69 -0.33191799703919145 2307 70-71 -0.3568713259566323 2307 72-73 -0.44236033392678564 2307 74-75 -0.4709250992334475 2307 76-77 -0.7937695742180075 2307 78-79 -0.3896642000866706 2307 80-81 -0.2844726221395888 2307 82-83 -0.3108525237323576 2307 84-85 -0.38168296907608834 2307 86-87 -0.22811201480557486 2307 88-89 -0.3497902346202153 2307 90-91 -0.378292474868239 2307 92-93 -0.34285452080255396 2307 94-95 -0.3932256033814028 2307 96-97 -0.5284799027933431 2307 98-99 -0.4198255502719448 2307 100-101 -0.6425854422671193 2201 1 0.10128626723817291 2201 2 0.0930435400586731 2201 3 0.07423531867296163 2201 4 0.05111854453056708 2201 5 0.06354955704031084 2201 6 0.07561892749151156 2201 7 0.08396587437957237 2201 8 0.08427727051445544 2201 9 0.07601089111713577 2201 10-11 0.07695131047066894 2201 12-13 0.0861612894189534 2201 14-15 0.18642808297898483 2201 16-17 0.17972950901544493 2201 18-19 0.1271820302749731 2201 20-21 0.16388088166645076 2201 22-23 0.17236530328129618 2201 24-25 0.09329785823076264 2201 26-27 0.048344630667010335 2201 28-29 0.08202725850651404 2201 30-31 0.01834582425126996 2201 32-33 0.06061357106562326 2201 34-35 0.04700150041119855 2201 36-37 0.007031401046376118 2201 38-39 0.01815950568072111 2201 40-41 0.07635227919465848 2201 42-43 0.09491104114398752 2201 44-45 0.00450149540081668 2201 46-47 0.020232224375874353 2201 48-49 0.008473484522859565 2201 50-51 -0.03793329425757719 2201 52-53 -0.0698790959404505 2201 54-55 0.00830621047273894 2201 56-57 -0.036137017941243244 2201 58-59 -0.08873900498989684 2201 60-61 -0.17018669567867306 2201 62-63 -0.14222491424940387 2201 64-65 -0.18440383154648998 2201 66-67 -0.20733769866757967 2201 68-69 -0.27912183534569124 2201 70-71 -0.30644618142500235 2201 72-73 -0.3390976969228561 2201 74-75 -0.4629617987036241 2201 76-77 -0.6243437607204214 2201 78-79 -0.2573904054431111 2201 80-81 -0.15219193532742992 2201 82-83 -0.11518769079331292 2201 84-85 -0.14334342576120918 2201 86-87 -0.17065414907781928 2201 88-89 -0.10785231866513811 2201 90-91 -0.17971532909096055 2201 92-93 -0.19786377625447926 2201 94-95 -0.23833180032901424 2201 96-97 -0.3625018092939669 2201 98-99 -0.2563103112869811 2201 100-101 -0.4062694269482847 1101 1 0.08114535871283124 1101 2 0.07009171815691673 1101 3 0.07375137058755854 1101 4 0.05542073607642095 1101 5 0.061024191553471496 1101 6 0.07540377042147384 1101 7 0.05108585242800956 1101 8 0.05216436691364379 1101 9 0.02921075155656183 1101 10-11 0.04294189909324331 1101 12-13 0.07137875433770802 1101 14-15 0.07914580710656338 1101 16-17 0.07740181371016064 1101 18-19 0.025626286229972095 1101 20-21 0.06489190244456466 1101 22-23 0.09185914734933931 1101 24-25 0.0645724418794984 1101 26-27 0.04007793294068307 1101 28-29 0.0930353360487004 1101 30-31 0.05895128195743382 1101 32-33 0.06756987824196159 1101 34-35 0.012886965784062454 1101 36-37 0.017199564911372534 1101 38-39 -0.036815691516927984 1101 40-41 -0.022425094458689898 1101 42-43 -7.140986310503195E-4 1101 44-45 -0.07387430325690048 1101 46-47 -0.06630332372277792 1101 48-49 -0.09676473347096959 1101 50-51 -0.07690716708268752 1101 52-53 -0.02801313896235058 1101 54-55 -0.08263460037645132 1101 56-57 -0.15773179707990437 1101 58-59 -0.1731106432353542 1101 60-61 -0.284829666010431 1101 62-63 -0.23666416316675765 1101 64-65 -0.16300035079543562 1101 66-67 -0.28098798113770584 1101 68-69 -0.2648048068273141 1101 70-71 -0.29785755158722793 1101 72-73 -0.29130860906146694 1101 74-75 -0.41875605936726856 1101 76-77 -0.9036133154908121 1101 78-79 -0.3306557786296267 1101 80-81 -0.09101162579325717 1101 82-83 -0.09286033875006439 1101 84-85 -0.10035915203688006 1101 86-87 -0.08873812769716949 1101 88-89 -0.22158368517037275 1101 90-91 -0.18312997084021987 1101 92-93 -0.13320543559770925 1101 94-95 -0.15710995262441685 1101 96-97 -0.2793947744104841 1101 98-99 -0.32013197613674294 1101 100-101 -0.41286871413415227 2304 1 0.10447304667034274 2304 2 0.05852432181239209 2304 3 0.03532533227769363 2304 4 0.008933026666582577 2304 5 0.03531199152870812 2304 6 0.03952134463884249 2304 7 0.060325681628107475 2304 8 0.07089230651843081 2304 9 0.04499649770092873 2304 10-11 0.07594260501247874 2304 12-13 0.06333359508612091 2304 14-15 0.10404855776761934 2304 16-17 0.07232906376339798 2304 18-19 0.07109672329088568 2304 20-21 0.07230670302510589 2304 22-23 0.10255423196317537 2304 24-25 0.07142190862138165 2304 26-27 0.0451710000044514 2304 28-29 0.029673732922233853 2304 30-31 0.04114759516175326 2304 32-33 0.057335432289995936 2304 34-35 0.08668085530383962 2304 36-37 0.07966325036968414 2304 38-39 0.02725762256509512 2304 40-41 0.001007305438903927 2304 42-43 0.03240726112453274 2304 44-45 -0.0883803224794093 2304 46-47 -0.02553435470599652 2304 48-49 -0.06556882287078025 2304 50-51 -0.06608480712986164 2304 52-53 -0.06806544346906662 2304 54-55 -0.04452579909690968 2304 56-57 -0.0580795315780378 2304 58-59 -0.12374025845413428 2304 60-61 -0.15143454148486768 2304 62-63 -0.17612859222818855 2304 64-65 -0.17469211404678475 2304 66-67 -0.18259697778509576 2304 68-69 -0.24550585900755806 2304 70-71 -0.2456108447245242 2304 72-73 -0.24833146110761817 2304 74-75 -0.315444005531873 2304 76-77 -0.8167282207061781 2304 78-79 -0.25014208647690594 2304 80-81 -0.04407612678935635 2304 82-83 -0.10997821388539109 2304 84-85 -0.14271779297127551 2304 86-87 -0.06293638651689548 2304 88-89 -0.11734380794342059 2304 90-91 -0.1505776412304627 2304 92-93 -0.08630225623208077 2304 94-95 -0.09386223096194612 2304 96-97 -0.1494534089061048 2304 98-99 -0.10144878783191302 2304 100-101 -0.21575870836138122 2202 1 0.07312442824210308 2202 2 0.07234186354327221 2202 3 0.07803727215857492 2202 4 0.020431375681020825 2202 5 0.02644100373127145 2202 6 0.013178274341228757 2202 7 0.02107496636723738 2202 8 0.06248782081020465 2202 9 0.05881063682505783 2202 10-11 0.081273859964611 2202 12-13 0.03425830213083003 2202 14-15 0.03199544858525627 2202 16-17 0.043773596083255484 2202 18-19 0.007690952379938665 2202 20-21 0.04696745921227574 2202 22-23 0.048817958593630806 2202 24-25 0.03556421688352884 2202 26-27 0.019438381400426863 2202 28-29 0.025898410066858446 2202 30-31 0.005499574037862942 2202 32-33 -0.005729607539684878 2202 34-35 -0.02604524307982814 2202 36-37 0.053364317265128136 2202 38-39 0.08472810787853291 2202 40-41 -0.01529367926503511 2202 42-43 0.07986305940737992 2202 44-45 0.0027410053722505268 2202 46-47 -0.04301623995418424 2202 48-49 -0.03685529339237803 2202 50-51 -0.08708076716416002 2202 52-53 -0.03892273865427143 2202 54-55 0.020804739164844932 2202 56-57 0.0015403218786431694 2202 58-59 1.0343287200953455E-5 2202 60-61 -0.10874225152728201 2202 62-63 -0.17483576369283327 2202 64-65 -0.08282922699360284 2202 66-67 -0.12625526152635302 2202 68-69 -0.255360704535434 2202 70-71 -0.1830292651370371 2202 72-73 -0.2253145225339921 2202 74-75 -0.32877034883423306 2202 76-77 -0.5906985477382918 2202 78-79 -0.2177535718416479 2202 80-81 0.01286660428665698 2202 82-83 0.022314470067918535 2202 84-85 -0.015450414220033792 2202 86-87 0.0917505746553644 2202 88-89 0.006258879712405019 2202 90-91 -0.0601994798024208 2202 92-93 -0.05966289781046186 2202 94-95 -0.04661670436799881 2202 96-97 -0.13533869834579093 2202 98-99 -0.14498018099783394 2202 100-101 0.06042815301676896 1102 1 0.07523760642201438 1102 2 0.06298816137882568 1102 3 0.03436006549527093 1102 4 0.03147145923038153 1102 5 0.020968367565629364 1102 6 0.05947186094562795 1102 7 0.0388300934545569 1102 8 0.07721191519091519 1102 9 0.06439246195934345 1102 10-11 0.035363078530366465 1102 12-13 0.0489552211973745 1102 14-15 0.07507672967678047 1102 16-17 0.04796751471615579 1102 18-19 0.05604117670893771 1102 20-21 0.1017484299843332 1102 22-23 0.09343520092663482 1102 24-25 0.061215221237681305 1102 26-27 0.0946704342952529 1102 28-29 0.04768337482975937 1102 30-31 -0.01656123089338024 1102 32-33 0.031246333450432928 1102 34-35 -0.012318407620973915 1102 36-37 -0.054642037505537644 1102 38-39 -0.022143911627914292 1102 40-41 2.57277209449569E-4 1102 42-43 -0.035569081638470834 1102 44-45 -0.04990415738183174 1102 46-47 -0.07644014498752938 1102 48-49 -0.07657341476388524 1102 50-51 -0.17894272721988358 1102 52-53 -0.15053760155734608 1102 54-55 -0.042643586390028076 1102 56-57 -0.048006084476739375 1102 58-59 -0.04232194868991712 1102 60-61 -0.12250754795660868 1102 62-63 -0.18442536241060026 1102 64-65 -0.18889825790999026 1102 66-67 -0.19248619878967332 1102 68-69 -0.20369381407397213 1102 70-71 -0.16532017318797187 1102 72-73 -0.1862744184226912 1102 74-75 -0.23450914798617362 1102 76-77 -0.3827682604370857 1102 78-79 -0.2255846468114342 1102 80-81 -0.12452699962761926 1102 82-83 -0.14870081586095552 1102 84-85 -0.1572401372965615 1102 86-87 -0.22959443289119008 1102 88-89 -0.28371372004065165 1102 90-91 -0.3947546211777464 1102 92-93 -0.26104218839117976 1102 94-95 -0.3896389020736919 1102 96-97 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48-49 -0.8601655430274278 1213 50-51 -0.5814814777324315 1213 52-53 -0.2224301976248313 1213 54-55 -0.8022549780096995 1213 56-57 -0.29359566222758104 1213 58-59 -0.08625567473436746 1213 60-61 -0.09156580948212678 1213 62-63 0.08222315468222519 1213 64-65 0.07402620385872893 1213 66-67 -0.2045784424732986 1213 68-69 0.08191807670542772 1213 70-71 0.09552143992691953 1213 72-73 0.18299898589627617 1213 74-75 0.18380072733325648 1213 76-77 0.7911722422112781 1213 78-79 0.062187629264514044 1213 80-81 -0.07252457994702866 1213 82-83 0.040796080159474 1213 84-85 0.014701418808932942 1213 86-87 -1.2470792732944673 1213 88-89 -0.49713792903563103 1213 90-91 -0.22222366284557893 1213 92-93 -0.09477080968460427 1213 94-95 0.18347240246392005 1213 96-97 0.30723468764526984 1213 98-99 0.19652966659985793 1213 100-101 0.6329509556811068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 29.0 8 378.0 9 1074.0 10 778.0 11 271.0 12 272.0 13 587.0 14 1535.0 15 3596.0 16 6543.0 17 10101.0 18 14247.0 19 18482.0 20 24075.0 21 30799.0 22 38781.0 23 49263.0 24 62046.0 25 78497.0 26 99702.0 27 126924.0 28 162697.0 29 206665.0 30 266825.0 31 349430.0 32 473798.0 33 675487.0 34 1059183.0 35 1983118.0 36 4266839.0 37 5344062.0 38 806255.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.68588481480589 21.122410614296122 31.59057220645294 27.601132364445057 2 27.47987596637156 21.299713637666606 32.33939829818496 18.88101209777687 3 20.856784123091686 19.546928421667314 40.208339224066535 19.38794823117446 4 20.446594961881743 19.694416123266425 39.50060947725521 20.358379437596618 5 29.813249784638916 19.02266193164118 32.45198065063517 18.712107633084734 6 29.8759942139749 19.608955135412824 32.37519126965806 18.139859380954213 7 29.945680685487687 26.598205673900598 24.683080615980643 18.773033024631072 8 26.20568330514959 21.50259203604219 32.41774685441034 19.873977804397878 9 22.38117491403345 20.25357510531703 36.45874549130757 20.906504489341955 10-11 28.242387516555468 21.316893339786198 28.769542196071434 21.6711769475869 12-13 26.847450684862007 26.193512341622956 24.360750087548986 22.59828688596605 14-15 26.61367986951931 23.51944709170815 26.134326288727948 23.732546750044595 16-17 25.93415230368746 23.288134808805687 25.56219472604409 25.215518161462764 18-19 25.787366485565162 24.169675761463758 26.27312382184047 23.76983393113061 20-21 24.393706522095236 24.775320106491954 26.898390224499995 23.932583146912815 22-23 24.597802342215623 24.86488538297154 26.08327271420085 24.45403956061199 24-25 25.02523605572963 24.703074563658312 25.84065996175937 24.43102941885269 26-27 24.862202275087846 24.675304104737222 26.20871737317706 24.253776246997873 28-29 24.99575403168999 25.101409809971802 25.36493208214057 24.537904076197638 30-31 25.207977559822154 25.06343094766973 25.227569497472214 24.501021995035906 32-33 25.047018105180108 25.46018098436054 25.246762685050744 24.246038225408604 34-35 25.008908026046395 25.507228478756637 25.186944802906947 24.29691869229002 36-37 25.061203763683043 25.34821604273502 25.397145023661842 24.193435169920086 38-39 24.908659127406292 25.017797613988957 25.560232320157628 24.513310938447123 40-41 24.60962261139467 25.351406552799165 25.200287635180135 24.838683200626033 42-43 24.496555544903085 25.26587529228424 25.343247493501092 24.894321669311584 44-45 24.85270900348004 25.04462351047687 25.44371064660531 24.658956839437778 46-47 24.318780861712145 25.248401344552274 25.568390799529624 24.864426994205964 48-49 24.619824139030438 24.894034337147456 25.340380436522935 25.145761087299174 50-51 24.345243095179864 25.113445861083562 26.084155881764648 24.457155161971926 52-53 24.543731030819487 25.078361186562926 26.017377390593214 24.36053039202437 54-55 24.432245250819722 25.0381405280524 25.298721318493072 25.230892902634807 56-57 24.416061892504338 25.44948483247626 25.72875964680203 24.405693628217374 58-59 24.46681256436504 25.590567895125453 25.582581540188816 24.360038000320692 60-61 25.159900106657307 25.251759112334465 25.02636885620192 24.561971924806304 62-63 24.548707423564302 25.681476192136486 25.580589974310676 24.189226409988535 64-65 24.90016001748503 25.191729833010406 25.493810097444268 24.41430005206029 66-67 25.051532281799275 25.588149405540285 25.45596472797766 23.90435358468278 68-69 24.899500162913327 25.02804835528715 26.126219409112174 23.946232072687348 70-71 24.657655592178646 25.707511982433296 25.590306752148734 24.04452567323933 72-73 24.608722274055374 25.242501025275377 25.943367052298093 24.205409648371155 74-75 24.618807142956385 25.25832584054899 25.769910473694875 24.352956542799753 76-77 24.555198437691015 25.039214017452178 25.686931601010798 24.71865594384601 78-79 24.845392723103704 24.98385097210821 25.367492287619054 24.80326401716903 80-81 24.618774269001793 24.805823236343745 25.82730698366881 24.748095510985653 82-83 24.435182941124314 25.452785771664566 25.80309198061651 24.30893930659461 84-85 24.58168282667501 25.30313223154585 25.661460631755613 24.453724310023524 86-87 24.41941201985629 25.394169567318254 25.809128869499748 24.37728954332571 88-89 24.4956014575749 26.66495208622277 24.978832811326924 23.860613644875407 90-91 24.488469205373107 25.57222684072099 25.194762282069856 24.74454167183605 92-93 24.271312369047994 25.953723573859833 25.296832800539253 24.478131256552917 94-95 24.31020926462312 26.064618193506146 25.013162956175144 24.61200958569559 96-97 24.25167024175953 25.396533318167837 25.336847017482672 25.01494942258996 98-99 24.521034082318383 25.092319717865607 25.354619435536303 25.032026764279703 100-101 24.914569749868992 25.87941243242245 24.782585689007416 24.42343212870114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17446.0 1 10690.5 2 5698.5 3 6537.0 4 5870.0 5 4701.0 6 3090.5 7 2942.5 8 3081.0 9 3216.0 10 3219.0 11 3092.0 12 2973.5 13 3092.5 14 3295.5 15 3517.5 16 3806.0 17 4153.0 18 4571.0 19 5087.5 20 5677.5 21 6403.0 22 7239.5 23 8136.0 24 9448.0 25 11159.0 26 13307.5 27 15665.0 28 18248.5 29 21269.0 30 24973.5 31 29932.0 32 36700.0 33 45879.5 34 58148.0 35 74189.5 36 96871.0 37 124179.0 38 151258.5 39 184524.0 40 238383.5 41 327731.0 42 423987.5 43 467352.5 44 518088.0 45 655312.5 46 818545.0 47 958716.5 48 1098804.0 49 1173183.0 50 1150448.0 51 1077533.5 52 985726.0 53 927428.0 54 858546.5 55 767779.5 56 672864.0 57 522726.5 58 388186.0 59 308553.5 60 236773.5 61 178724.5 62 133027.5 63 89596.5 64 51961.5 65 30523.5 66 19220.5 67 12591.0 68 9067.0 69 6550.0 70 4912.0 71 3692.5 72 2886.5 73 2217.5 74 1659.0 75 1183.0 76 830.5 77 633.0 78 512.5 79 400.5 80 286.5 81 195.0 82 120.0 83 80.5 84 54.5 85 32.5 86 30.5 87 30.5 88 24.0 89 22.5 90 20.0 91 17.0 92 12.0 93 8.5 94 8.0 95 9.5 96 8.5 97 4.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16018669514925113 2 0.0 3 0.0 4 0.08232692798980053 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.5267059186973017E-4 12-13 6.805923702749178E-5 14-15 0.0 16-17 3.9598101543267944E-4 18-19 0.0 20-21 0.0014261503758942597 22-23 1.2374406732271233E-5 24-25 0.0 26-27 7.053411837394603E-4 28-29 0.0 30-31 8.074300392806981E-4 32-33 0.0 34-35 0.0 36-37 0.001178662241248835 38-39 0.0050394771417174595 40-41 0.0058561879860473615 42-43 0.00158083046004765 44-45 0.0031369121066307576 46-47 0.019232921663632566 48-49 0.009983052631259818 50-51 0.009209652210492867 52-53 0.012170229021188759 54-55 0.010889477924398686 56-57 0.015480382822071313 58-59 0.022150188050765508 60-61 0.010855448305884939 62-63 0.0038329724853210147 64-65 0.014119198081521478 66-67 0.026700876126558252 68-69 0.031099977719880677 70-71 0.014082074861324664 72-73 0.01298384626383559 74-75 0.02516644969175662 76-77 0.04217197814358036 78-79 0.007433924844411944 80-81 0.001936594653600448 82-83 0.0024006349060606193 84-85 0.012841540586414473 86-87 0.01467913998615675 88-89 0.018233688320001662 90-91 0.009503544370384307 92-93 0.019078241579479172 94-95 0.026954551464569817 96-97 0.017967638575257832 98-99 0.008034083570927098 100-101 0.32008568534197696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6162391E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.992695847814023 #Duplication Level Percentage of deduplicated Percentage of total 1 79.69395108610841 19.120727293195674 2 8.36460694699048 4.013789407313096 3 3.436246408652033 2.473344448227944 4 1.8584709495934264 1.7835891294237276 5 0.9402798019141493 1.1279923649584502 6 0.6798070535066818 0.9786242321990669 7 0.5348493822204662 0.8982734986404813 8 0.3660932471680499 0.70268511449933 9 0.2934870709232872 0.6337391425135428 >10 2.7337651743170523 13.822626407614772 >50 0.4937969671727151 8.263150864689537 >100 0.5298421218624919 26.431775109789662 >500 0.046187274055336805 7.527851567144282 >1k 0.028163995959266042 11.40740531000194 >5k 3.489234412954027E-4 0.5372164983031896 >10k+ 1.0359611487452007E-4 0.27720961148536033 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0518245722430549E-4 0.0 0.0 2 1.0518245722430549E-4 0.0 0.0 3 1.1136966059044111E-4 0.0 0.0 4 1.1136966059044111E-4 0.0 0.0 5 1.1755686395657673E-4 0.0 0.0 6 1.2993127068884795E-4 0.0 0.0 7 1.6086728751952604E-4 0.0 0.0 8 1.9180330435020413E-4 0.0 0.0 9 2.165521178147466E-4 0.0 0.0 10-11 2.6914334642689933E-4 0.0 0.0 12-13 3.8051300701734045E-4 0.0 0.0 14-15 5.290058878045952E-4 0.0 0.0 16-17 6.898731753241212E-4 0.0 0.0 18-19 9.249869032372747E-4 0.0 1.856161009840685E-5 20-21 0.0013209679186699543 0.0 1.856161009840685E-5 22-23 0.0016396088920259387 0.0 1.856161009840685E-5 24-25 0.0021098363478522456 0.0 1.856161009840685E-5 26-27 0.002731650286148875 0.0 1.856161009840685E-5 28-29 0.0033627450294947078 0.0 1.856161009840685E-5 30-31 0.0040464310014526935 0.0 1.856161009840685E-5 32-33 0.0048878906592471376 0.0 1.856161009840685E-5 34-35 0.0057788479439706665 0.0 1.856161009840685E-5 36-37 0.0067688004825523646 0.0 1.856161009840685E-5 38-39 0.00789177789350598 1.546800841533904E-5 1.856161009840685E-5 40-41 0.009197277803760595 1.856161009840685E-5 1.856161009840685E-5 42-43 0.010397595256790904 1.856161009840685E-5 1.856161009840685E-5 44-45 0.011990800123570825 1.856161009840685E-5 1.856161009840685E-5 46-47 0.013887177955291392 1.856161009840685E-5 1.856161009840685E-5 48-49 0.016000107904826706 1.856161009840685E-5 1.856161009840685E-5 50-51 0.018326496370493696 1.856161009840685E-5 1.856161009840685E-5 52-53 0.021240669155943574 1.856161009840685E-5 1.856161009840685E-5 54-55 0.024541542151776924 1.856161009840685E-5 1.856161009840685E-5 56-57 0.028686968407087787 2.165521178147466E-5 1.856161009840685E-5 58-59 0.03339233656703393 2.474881346454247E-5 1.856161009840685E-5 60-61 0.03894844518982371 2.474881346454247E-5 1.856161009840685E-5 62-63 0.045457383130998376 2.474881346454247E-5 1.856161009840685E-5 64-65 0.053380097041335034 2.7842415147610274E-5 1.856161009840685E-5 66-67 0.06298573026726058 3.71232201968137E-5 1.856161009840685E-5 68-69 0.07447227331649135 3.71232201968137E-5 1.856161009840685E-5 70-71 0.0883532640684166 4.021682187988151E-5 1.856161009840685E-5 72-73 0.10519792523272083 4.331042356294932E-5 1.856161009840685E-5 74-75 0.12555073070562395 4.331042356294932E-5 1.856161009840685E-5 76-77 0.15012011527254848 4.331042356294932E-5 1.856161009840685E-5 78-79 0.17976610020138728 5.877843197828836E-5 1.856161009840685E-5 80-81 0.2165304626029651 6.805923702749178E-5 1.856161009840685E-5 82-83 0.26068234582370886 6.805923702749178E-5 1.856161009840685E-5 84-85 0.31339422490150126 6.805923702749178E-5 1.856161009840685E-5 86-87 0.3763954231771772 7.115283871055959E-5 1.856161009840685E-5 88-89 0.4489558506535326 7.42464403936274E-5 1.856161009840685E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 180680 0.0 72.399124 1 TTTGTGT 196580 0.0 66.63286 2 TTGTGTT 225630 0.0 59.707165 3 TAACTTT 17650 0.0 58.10991 1 TGTGTTT 236355 0.0 57.27129 4 AACTTTG 18060 0.0 57.17781 2 ACTTTGT 41405 0.0 53.070686 1 GTGTTTG 263160 0.0 51.435318 5 TTTGAGG 78065 0.0 37.33233 8 GTTTGAG 204910 0.0 36.4022 7 TTGAGGG 40280 0.0 35.64568 9 TGTTTGA 380875 0.0 35.514793 6 TTTGAGC 52040 0.0 34.22349 8 TTGAGGC 13545 0.0 33.826954 9 GTTTGAC 72965 0.0 30.981197 7 TTGAGGT 23230 0.0 30.55671 9 GTTTGAA 28505 0.0 29.991327 2 TTTGACC 18890 0.0 29.056309 8 TTGAGCC 29255 0.0 28.937819 9 TTTGAGT 79975 0.0 28.640348 3 >>END_MODULE