FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_8561.34100000035d01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_8561.34100000035d01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16162391
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1806800.072.3991241
TTTGTGT1965800.066.632862
TTGTGTT2256300.059.7071653
TAACTTT176500.058.109911
TGTGTTT2363550.057.271294
AACTTTG180600.057.177812
ACTTTGT414050.053.0706861
GTGTTTG2631600.051.4353185
TTTGAGG780650.037.332338
GTTTGAG2049100.036.40227
TTGAGGG402800.035.645689
TGTTTGA3808750.035.5147936
TTTGAGC520400.034.223498
TTGAGGC135450.033.8269549
GTTTGAC729650.030.9811977
TTGAGGT232300.030.556719
GTTTGAA285050.029.9913272
TTTGACC188900.029.0563098
TTGAGCC292550.028.9378199
TTTGAGT799750.028.6403483