##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_7153.34100000035ccc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15494087 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73781869173705 33.0 33.0 33.0 33.0 33.0 2 32.76488650154088 33.0 33.0 33.0 33.0 33.0 3 32.79009831298869 33.0 33.0 33.0 33.0 33.0 4 36.70629776378563 37.0 37.0 37.0 37.0 37.0 5 36.71806405888905 37.0 37.0 37.0 37.0 37.0 6 36.66769632828317 37.0 37.0 37.0 37.0 37.0 7 36.62825612118997 37.0 37.0 37.0 37.0 37.0 8 36.62058235506229 37.0 37.0 37.0 37.0 37.0 9 36.60785098211982 37.0 37.0 37.0 37.0 37.0 10-11 36.59863081961525 37.0 37.0 37.0 37.0 37.0 12-13 36.563995832732836 37.0 37.0 37.0 37.0 37.0 14-15 38.645972234440144 40.0 37.0 40.0 37.0 40.0 16-17 38.6018179064052 40.0 37.0 40.0 37.0 40.0 18-19 38.551502131103305 40.0 37.0 40.0 37.0 40.0 20-21 38.50094939443673 40.0 37.0 40.0 37.0 40.0 22-23 38.45280499586713 40.0 37.0 40.0 37.0 40.0 24-25 38.41109847259797 40.0 37.0 40.0 37.0 40.0 26-27 38.313302164883936 40.0 37.0 40.0 37.0 40.0 28-29 38.24038273439409 40.0 37.0 40.0 37.0 40.0 30-31 38.18067860339238 40.0 37.0 40.0 37.0 40.0 32-33 38.08508300618165 40.0 37.0 40.0 37.0 40.0 34-35 37.989507545685015 40.0 37.0 40.0 37.0 40.0 36-37 37.878552088935606 40.0 37.0 40.0 33.0 40.0 38-39 37.7467881779675 40.0 37.0 40.0 33.0 40.0 40-41 37.58755559459554 40.0 37.0 40.0 33.0 40.0 42-43 37.43708154601171 40.0 37.0 40.0 33.0 40.0 44-45 37.26360462542904 38.5 37.0 40.0 33.0 40.0 46-47 37.04401582358483 37.0 37.0 40.0 33.0 40.0 48-49 36.79505200919551 37.0 37.0 40.0 33.0 40.0 50-51 36.55398604641887 37.0 37.0 40.0 33.0 40.0 52-53 36.29821828159349 37.0 37.0 40.0 33.0 40.0 54-55 35.997248014678114 37.0 37.0 40.0 33.0 40.0 56-57 35.73711677880729 37.0 35.0 38.5 33.0 40.0 58-59 35.451716580654285 37.0 33.0 37.0 33.0 40.0 60-61 35.162297591332745 37.0 33.0 37.0 33.0 40.0 62-63 34.9048764215665 37.0 33.0 37.0 27.0 40.0 64-65 34.64032117542647 37.0 33.0 37.0 27.0 38.5 66-67 34.41612881094575 37.0 33.0 37.0 27.0 37.0 68-69 34.18308665105598 37.0 33.0 37.0 27.0 37.0 70-71 33.96395482999418 37.0 33.0 37.0 27.0 37.0 72-73 33.76007473044395 37.0 33.0 37.0 27.0 37.0 74-75 33.51307811812339 37.0 33.0 37.0 27.0 37.0 76-77 30.627796720129428 33.0 30.0 33.0 24.5 35.0 78-79 33.307914270779555 35.0 33.0 37.0 27.0 37.0 80-81 33.85328499833517 37.0 33.0 37.0 27.0 37.0 82-83 33.828406927107096 37.0 33.0 37.0 27.0 37.0 84-85 33.76308423335948 37.0 33.0 37.0 27.0 37.0 86-87 33.613575294885074 37.0 33.0 37.0 27.0 37.0 88-89 33.47372536374682 37.0 33.0 37.0 27.0 37.0 90-91 33.386813563135405 37.0 33.0 37.0 27.0 37.0 92-93 33.291432563919386 37.0 33.0 37.0 27.0 37.0 94-95 33.1913684878625 37.0 33.0 37.0 27.0 37.0 96-97 33.05984211912583 33.0 33.0 37.0 27.0 37.0 98-99 32.87220202132594 33.0 33.0 37.0 27.0 37.0 100-101 30.847468037322884 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10965681421495788 2306 2 0.09574990345424794 2306 3 0.06728841996453383 2306 4 0.08292106013073663 2306 5 0.0722201741952091 2306 6 0.05926596484331981 2306 7 0.10817645797261832 2306 8 0.10216876205640801 2306 9 0.09061849496264074 2306 10-11 0.08785179775187402 2306 12-13 0.09709136004303076 2306 14-15 0.1734558499231511 2306 16-17 0.16361384247916533 2306 18-19 0.18236642437346973 2306 20-21 0.20192658543159325 2306 22-23 0.17892773866820022 2306 24-25 0.12065187402412647 2306 26-27 0.14537423096874846 2306 28-29 0.10139683646526265 2306 30-31 0.044682900708693296 2306 32-33 0.05914265973516564 2306 34-35 0.09414958267424822 2306 36-37 0.08836272986136606 2306 38-39 0.011668786742326631 2306 40-41 0.012242815855856293 2306 42-43 0.06942584311571665 2306 44-45 -0.0229714638532883 2306 46-47 0.003095418331255928 2306 48-49 -0.021594577453981856 2306 50-51 -0.04978459283473313 2306 52-53 -0.04107069419486464 2306 54-55 0.02594398774245832 2306 56-57 -0.05146326622263331 2306 58-59 -0.08993489112058484 2306 60-61 -0.2277518153083662 2306 62-63 -0.2838344525260439 2306 64-65 -0.2468085423882158 2306 66-67 -0.2830805255829105 2306 68-69 -0.28482665807002405 2306 70-71 -0.3317265919531991 2306 72-73 -0.39911122893115447 2306 74-75 -0.4943173864081416 2306 76-77 -0.31666513468903545 2306 78-79 -0.2747376215429682 2306 80-81 -0.2590401397236022 2306 82-83 -0.32925354139790386 2306 84-85 -0.429616398263839 2306 86-87 -0.33160808061016667 2306 88-89 -0.5109827996842284 2306 90-91 -0.551295184318171 2306 92-93 -0.5099266488155578 2306 94-95 -0.5263719988113635 2306 96-97 -0.5015819412378804 2306 98-99 -0.4659925506900393 2306 100-101 -0.7990694087974148 2307 1 0.10798573659449318 2307 2 0.09440215017825437 2307 3 0.08900540796481948 2307 4 0.06825484245222668 2307 5 0.08012975304382763 2307 6 0.08756385894427154 2307 7 0.1085250524689414 2307 8 0.08016047088831613 2307 9 0.10620380426645681 2307 10-11 0.09095507686249249 2307 12-13 0.08857909944290299 2307 14-15 0.16079833572269564 2307 16-17 0.11929770295905229 2307 18-19 0.15656017719397397 2307 20-21 0.17686442199504882 2307 22-23 0.11939476433210672 2307 24-25 0.13708402122181695 2307 26-27 0.10477103784486275 2307 28-29 0.11424064697220615 2307 30-31 0.07473450142073546 2307 32-33 0.042096201257407984 2307 34-35 0.057291783503792715 2307 36-37 0.0775158511770897 2307 38-39 0.02754930770199593 2307 40-41 0.022953927877843228 2307 42-43 0.015520192624485674 2307 44-45 -0.011813974070214783 2307 46-47 -0.04104598690067007 2307 48-49 -0.09371479848167752 2307 50-51 -0.04517557580782494 2307 52-53 -0.075668840082038 2307 54-55 -0.09378452071823773 2307 56-57 -0.12676132610032909 2307 58-59 -0.11246991145780072 2307 60-61 -0.21657044466329012 2307 62-63 -0.2900742648742707 2307 64-65 -0.3090853428481637 2307 66-67 -0.35913872953629067 2307 68-69 -0.3804554682773684 2307 70-71 -0.4146634044359345 2307 72-73 -0.5582429358540182 2307 74-75 -0.6487720863778179 2307 76-77 -0.8259756364638591 2307 78-79 -0.3690828416354748 2307 80-81 -0.34357781916867935 2307 82-83 -0.33361753886190826 2307 84-85 -0.4461068707628044 2307 86-87 -0.35487198777814655 2307 88-89 -0.4447216900556654 2307 90-91 -0.4899233196504369 2307 92-93 -0.48468878818149363 2307 94-95 -0.5560417828786299 2307 96-97 -0.5220901013934522 2307 98-99 -0.37087849191252076 2307 100-101 -0.7349006265253877 2201 1 0.09525522512173268 2201 2 0.09530235562350242 2201 3 0.07148077772299644 2201 4 0.03878152084192976 2201 5 0.07721046510513929 2201 6 0.06936715179586628 2201 7 0.08698155179344269 2201 8 0.10137843525137669 2201 9 0.07405529185313497 2201 10-11 0.08554473120454276 2201 12-13 0.09691375870630026 2201 14-15 0.16065669874208055 2201 16-17 0.15701764283323882 2201 18-19 0.11796196782468371 2201 20-21 0.13894379272093005 2201 22-23 0.16410347655284596 2201 24-25 0.11286798449749114 2201 26-27 0.07940881850614545 2201 28-29 0.07927494279007874 2201 30-31 0.056438272713137394 2201 32-33 0.09878169515148727 2201 34-35 0.02441463623173945 2201 36-37 0.02359946961654913 2201 38-39 0.043230276265326495 2201 40-41 0.07307222105374933 2201 42-43 0.1026869364871672 2201 44-45 0.041175901612213295 2201 46-47 0.011440841244152011 2201 48-49 0.057461274995397105 2201 50-51 -0.0428333412717663 2201 52-53 -0.031042946174537178 2201 54-55 0.05187122347260953 2201 56-57 -0.08234680071721812 2201 58-59 -0.08449117973226095 2201 60-61 -0.12558741983621502 2201 62-63 -0.17681680372597697 2201 64-65 -0.1736513807639568 2201 66-67 -0.20202015072886326 2201 68-69 -0.2924154071214531 2201 70-71 -0.2721147812268683 2201 72-73 -0.29340567489142444 2201 74-75 -0.46137743503901163 2201 76-77 -0.670223644977451 2201 78-79 -0.2945467370584325 2201 80-81 -0.18930086588668615 2201 82-83 -0.12216370837015944 2201 84-85 -0.15829831552719753 2201 86-87 -0.10368300852238832 2201 88-89 -0.08933871407873539 2201 90-91 -0.18297928608996727 2201 92-93 -0.1559228520426288 2201 94-95 -0.1790250879943187 2201 96-97 -0.3118146645337774 2201 98-99 -0.25178247158341804 2201 100-101 -0.3563980273106395 1101 1 0.07183830604815 1101 2 0.06774801844330369 1101 3 0.06503109991508893 1101 4 0.03563061940556622 1101 5 0.03038591707281313 1101 6 0.04456316869841004 1101 7 0.053399332958498746 1101 8 0.06547707953619408 1101 9 0.06209886418760391 1101 10-11 0.055243288364287935 1101 12-13 0.07682438418535043 1101 14-15 0.09430026413327397 1101 16-17 0.06131290934327893 1101 18-19 0.051459117265174825 1101 20-21 0.08363728879446342 1101 22-23 0.08836796335371133 1101 24-25 0.06195669312919705 1101 26-27 0.11008775175730534 1101 28-29 0.07394580558754171 1101 30-31 0.04999883897144741 1101 32-33 0.0291965528358773 1101 34-35 0.016504031752077708 1101 36-37 0.0077176223943169475 1101 38-39 -0.023975814164366227 1101 40-41 -0.05813716466072805 1101 42-43 -0.08858973484797872 1101 44-45 -0.10375878704121533 1101 46-47 -0.13806003860766225 1101 48-49 -0.12215984057767315 1101 50-51 -0.07445103439147971 1101 52-53 -0.023392327740630492 1101 54-55 -0.10587617956463902 1101 56-57 -0.16195031245793956 1101 58-59 -0.22396876141012712 1101 60-61 -0.24277040313163667 1101 62-63 -0.18493414131798858 1101 64-65 -0.16196828511114347 1101 66-67 -0.2506532845992737 1101 68-69 -0.26293232675555345 1101 70-71 -0.3241048669160804 1101 72-73 -0.36464165502481904 1101 74-75 -0.39901382631672533 1101 76-77 -0.9131241937806962 1101 78-79 -0.3302128252917953 1101 80-81 -0.11562165725533191 1101 82-83 -0.13828740720929744 1101 84-85 -0.17579848516530916 1101 86-87 -0.12925786539647532 1101 88-89 -0.20102511052805028 1101 90-91 -0.19494753838822731 1101 92-93 -0.15853256688031792 1101 94-95 -0.1223716639394965 1101 96-97 -0.234009834371129 1101 98-99 -0.1130844334168799 1101 100-101 -0.3483897332208201 2304 1 0.07669699732282709 2304 2 0.07288590739338474 2304 3 0.05076026320271154 2304 4 0.03588724839107016 2304 5 0.047217139246626516 2304 6 0.05411543446965794 2304 7 0.0734681544990039 2304 8 0.09478215215566621 2304 9 0.08021062086341857 2304 10-11 0.06693893443679144 2304 12-13 0.07162635232831605 2304 14-15 0.12020305371468254 2304 16-17 0.1210683448840868 2304 18-19 0.10462942451786006 2304 20-21 0.12131707190640384 2304 22-23 0.1340291577612689 2304 24-25 0.11794782145379656 2304 26-27 0.14123754666359645 2304 28-29 0.08725812411178424 2304 30-31 0.0990016932625366 2304 32-33 0.10454278997971045 2304 34-35 0.1156260478420279 2304 36-37 0.12477733340252684 2304 38-39 0.08765271804552555 2304 40-41 0.08297439988659505 2304 42-43 0.09711342730538064 2304 44-45 0.003482297567593662 2304 46-47 0.037116267497076194 2304 48-49 0.024272036288493837 2304 50-51 0.009495013829443621 2304 52-53 -0.01833558589989792 2304 54-55 0.03107586641821314 2304 56-57 0.017270724200947996 2304 58-59 -0.006724935854556691 2304 60-61 -0.05376926300031215 2304 62-63 -0.10816400251140834 2304 64-65 -0.1443475447561795 2304 66-67 -0.10116162076916879 2304 68-69 -0.16445992359342654 2304 70-71 -0.12620711091855696 2304 72-73 -0.15756540729520196 2304 74-75 -0.25999656548215455 2304 76-77 -0.8380603451596862 2304 78-79 -0.24527699270291237 2304 80-81 -0.05060549545114412 2304 82-83 -0.07461676653811367 2304 84-85 -0.07791616485532415 2304 86-87 -0.024236476259439144 2304 88-89 -0.1475068653774585 2304 90-91 -0.17359674749560128 2304 92-93 -0.04906489244797996 2304 94-95 -0.033054921598484555 2304 96-97 -0.10939757904807124 2304 98-99 -0.07247429742931644 2304 100-101 -0.22225850783848244 2202 1 0.09723070879004325 2202 2 0.06680905599777986 2202 3 0.07064033812037707 2202 4 0.018825774175567744 2202 5 0.022914330594751675 2202 6 0.014032028701116417 2202 7 0.03888794569433429 2202 8 0.06704846184998559 2202 9 0.033226327546138634 2202 10-11 0.06558259385747789 2202 12-13 0.04452006475072068 2202 14-15 0.0487985818183958 2202 16-17 0.07307263456967661 2202 18-19 0.042395739608814154 2202 20-21 0.12896633359853382 2202 22-23 0.10871732564788061 2202 24-25 0.06691409368480805 2202 26-27 0.08960917628722598 2202 28-29 0.0330432644722265 2202 30-31 0.03898077331383121 2202 32-33 0.019082853375969933 2202 34-35 -0.033435210843421714 2202 36-37 0.01767677017787861 2202 38-39 0.050869796570886194 2202 40-41 0.016653389402968344 2202 42-43 0.06940425955504992 2202 44-45 0.03908914021616994 2202 46-47 -0.017815875488494726 2202 48-49 0.07333550396125332 2202 50-51 0.017690339981392356 2202 52-53 0.03780224752646433 2202 54-55 0.04834759148686629 2202 56-57 0.028754958434802802 2202 58-59 0.008780234098502149 2202 60-61 -0.06512196182637098 2202 62-63 -0.09299167102260952 2202 64-65 -0.08190447860796723 2202 66-67 -0.09705734750837536 2202 68-69 -0.18668969272293623 2202 70-71 -0.08986281043856792 2202 72-73 -0.14847282964357333 2202 74-75 -0.30736435857016886 2202 76-77 -0.5717190595279469 2202 78-79 -0.14019393673729752 2202 80-81 0.07852187570799884 2202 82-83 0.0669001629449184 2202 84-85 0.002431378194344802 2202 86-87 0.09266033333094725 2202 88-89 0.0599723658077167 2202 90-91 -0.033667229849505986 2202 92-93 -0.013133583117458159 2202 94-95 -0.020476897510235403 2202 96-97 -0.17042318836925574 2202 98-99 -0.09267985404463275 2202 100-101 -0.04521185732594191 1102 1 0.04840632146319024 1102 2 0.044781861765699205 1102 3 0.048961214293498756 1102 4 -0.019100964500125883 1102 5 0.008968259807126344 1102 6 0.031721453657951315 1102 7 0.05237619325149723 1102 8 0.041558077759994205 1102 9 0.06535528714723426 1102 10-11 0.03927127705392053 1102 12-13 0.05727945600861517 1102 14-15 0.0716048594162757 1102 16-17 0.07900478419466594 1102 18-19 0.0553875432276314 1102 20-21 0.0946531938745423 1102 22-23 0.0941227037833059 1102 24-25 0.08842059516609169 1102 26-27 0.11604883545127365 1102 28-29 0.11647757521899393 1102 30-31 0.05758628428712598 1102 32-33 0.05639215784206186 1102 34-35 0.0352886682431901 1102 36-37 0.022233459029642688 1102 38-39 0.03515893656388869 1102 40-41 0.07813296978360285 1102 42-43 0.039850959205338654 1102 44-45 0.017157693210208436 1102 46-47 0.04220375788641917 1102 48-49 0.022457526361186808 1102 50-51 -0.0690134605442978 1102 52-53 -0.06377220787655347 1102 54-55 0.027626735307244132 1102 56-57 0.008170207479999192 1102 58-59 -0.011641878700210384 1102 60-61 -0.04049632209910925 1102 62-63 -0.026894876565954462 1102 64-65 -0.052591892462281464 1102 66-67 -0.08389597488493195 1102 68-69 -0.07387649097451998 1102 70-71 -0.06649883893478403 1102 72-73 -0.08943473157766846 1102 74-75 -0.1943783173148148 1102 76-77 -0.3349764331684817 1102 78-79 -0.16648625584694088 1102 80-81 -0.0026906781523408085 1102 82-83 0.0063630890067329915 1102 84-85 -0.0365078687555922 1102 86-87 -0.11112740600271565 1102 88-89 -0.13757144560596402 1102 90-91 -0.2249998288321038 1102 92-93 -0.14172526054615986 1102 94-95 -0.2558205965881868 1102 96-97 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1213 52-53 -0.21575451411211333 1213 54-55 -0.7768838223335592 1213 56-57 -0.2803970189341385 1213 58-59 -0.09596721731753632 1213 60-61 -0.07043166363893505 1213 62-63 0.07898160571429713 1213 64-65 0.05895135539179819 1213 66-67 -0.2650198507938839 1213 68-69 0.059402303721597605 1213 70-71 0.031522067915339846 1213 72-73 0.09630006200531938 1213 74-75 0.1670832064030634 1213 76-77 0.7611166222636747 1213 78-79 0.0744859512411864 1213 80-81 -0.0839367630772685 1213 82-83 0.007953985524693508 1213 84-85 -0.03971033729948914 1213 86-87 -1.2676211639551056 1213 88-89 -0.5471669488506237 1213 90-91 -0.27746197449160803 1213 92-93 -0.19825461390009735 1213 94-95 0.09378100872530837 1213 96-97 0.19636284620174393 1213 98-99 0.14923736224942985 1213 100-101 0.5979646727348253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 40.0 8 407.0 9 1205.0 10 855.0 11 315.0 12 317.0 13 631.0 14 1543.0 15 3255.0 16 5831.0 17 8696.0 18 11935.0 19 16234.0 20 21005.0 21 26928.0 22 34345.0 23 43717.0 24 55587.0 25 70661.0 26 89573.0 27 114268.0 28 146453.0 29 187562.0 30 245012.0 31 326032.0 32 448940.0 33 657802.0 34 1058225.0 35 1998998.0 36 4250357.0 37 5021249.0 38 646072.0 39 36.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.09121942478504 21.908288770537435 30.484915016306868 26.515576788370655 2 28.019114646768152 21.97517026979389 29.49063729924842 20.515077784189543 3 22.216249334342837 20.226922696380882 36.3878555735488 21.16897239572748 4 22.032809177728083 20.438335048207126 35.5242549729779 22.00460080108689 5 28.647386580441946 19.669361608722088 31.082922149591646 20.600329661244317 6 30.061435694791182 20.218074159516465 29.725242926543526 19.995247219148826 7 28.688466767999948 25.989120882050038 24.790069915058563 20.53234243489145 8 26.940509627963234 21.74625068259911 29.749168182675106 21.56407150676255 9 23.44554409691904 20.91461729884439 33.27462921823015 22.365209386006416 10-11 27.893353050163356 21.573051416741627 27.57880304806255 22.954792485032467 12-13 26.948485764866337 25.153538515186757 24.346868946981125 23.551106772965785 14-15 26.63988849423654 23.20048932215238 25.766390752807826 24.393231430803247 16-17 26.23617269175903 22.909367144652073 25.351257234846535 25.503202928742358 18-19 26.197416472490442 23.538992649260326 25.753247029011778 24.510343849237454 20-21 25.237109840960372 24.10699867573468 25.99698758360885 24.6589038996961 22-23 25.443707184673325 24.047171157847867 25.653887371804718 24.855234285674086 24-25 25.419158289223496 24.09542427378909 25.40766358159729 25.07775385539012 26-27 25.41637609691761 24.09583783642491 25.42836139314708 25.0594246735104 28-29 25.335956398591165 24.44079229840365 25.12901938426638 25.094231918738803 30-31 25.545168293460097 24.125590866696136 25.073930340802754 25.255310499041016 32-33 25.381553621068477 24.34399006537139 25.108617242177612 25.16583907138252 34-35 25.319607408942524 24.574213375721975 24.83102747519102 25.27515174014448 36-37 25.499191714001974 24.31001058029494 25.062661769340533 25.128135936362554 38-39 25.511220684032494 24.201291856481433 24.915207617855337 25.37227984163074 40-41 25.44037387312042 24.529433791488966 24.88162768233913 25.148564653051487 42-43 25.492225919286366 24.422170625324263 24.878090953070306 25.20751250231907 44-45 25.613791319842022 24.236458142831292 25.09833334091711 25.051417196409574 46-47 25.328633860732936 24.47993686697523 25.06110282678441 25.13032644550742 48-49 25.45365535047912 24.25024949862258 25.034270458862846 25.26182469203545 50-51 25.26916756069143 24.617501447297055 25.297645538636232 24.815685453375284 52-53 25.316034454674373 24.4760783981363 25.3073010159739 24.90058613121543 54-55 25.285296799231883 24.434581528009748 25.000692276039022 25.279429396719355 56-57 25.3700171043397 24.519289625255357 25.162692948296613 24.94800032210833 58-59 25.21657824417416 24.530326183164995 25.1927734713285 25.06032210133235 60-61 25.61310549773473 24.567679931300503 24.79831561270788 25.020898958256883 62-63 25.18914811872956 24.86274629272269 25.14842453562481 24.799681052922942 64-65 25.42253917972625 24.755912404933245 24.88543189440505 24.936116520935457 66-67 25.597754615094324 24.88905113075093 24.76117430154755 24.7520199526072 68-69 25.41312100339158 24.783409139784705 24.977653823525216 24.825816033298498 70-71 25.323149300667 24.86331669080089 24.808781504422363 25.004752504109746 72-73 25.43153554585152 24.59485861823033 24.898062304298957 25.075543531619193 74-75 25.170957314479857 24.611813953200116 25.040228858077572 25.17699987424245 76-77 25.327686209485954 24.461854149757905 24.864384443484532 25.34607519727161 78-79 25.40613484229276 24.458041511186686 24.928560750858264 25.207262895662293 80-81 25.242601456307405 24.47004927882168 25.057230240621468 25.230119024249447 82-83 25.232305032019 24.917806063196753 24.88453768425685 24.9653512205274 84-85 25.242613569925705 24.90365638249416 24.76871318944406 25.085016858136072 86-87 25.059843053471027 24.99597044185969 24.873841102629118 25.070345402040168 88-89 25.03009203888899 25.461513740344653 24.543597218128056 24.9647970026383 90-91 25.039855365614827 24.982473062994696 24.77323098155075 25.20444058983972 92-93 24.940195427930583 25.31266780824825 24.560564089731244 25.18657267408993 94-95 25.084274573714232 25.368183178603992 24.319144208295448 25.228398039386324 96-97 25.044426626527077 25.040424373129312 24.426068793700814 25.4890802066428 98-99 25.077516462768152 25.169249171778596 24.538903072503565 25.214331292949684 100-101 25.500930754349678 25.488865360237316 23.895883709671978 25.114320175741028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 40938.0 1 23438.5 2 9480.0 3 11493.5 4 10446.0 5 8086.0 6 4770.5 7 4589.5 8 4966.0 9 4863.0 10 4228.0 11 3463.5 12 2987.0 13 2758.5 14 2706.0 15 2754.5 16 2895.0 17 3103.0 18 3356.5 19 3669.0 20 4046.0 21 4402.0 22 4875.5 23 5447.5 24 6196.0 25 7225.5 26 8166.0 27 9298.5 28 10645.5 29 11997.0 30 13836.5 31 16815.5 32 24525.0 33 37989.0 34 56921.0 35 76799.0 36 98001.0 37 132856.5 38 172159.0 39 196131.5 40 210658.0 41 232215.0 42 272455.0 43 324379.0 44 391043.0 45 481725.0 46 586196.5 47 697999.0 48 810877.5 49 899759.5 50 911509.0 51 886548.5 52 888817.0 53 918588.5 54 923792.0 55 905561.5 56 878284.0 57 776468.0 58 640357.5 59 522017.0 60 420823.0 61 317524.0 62 212709.5 63 132403.5 64 78560.5 65 47403.0 66 28002.5 67 16415.0 68 10956.0 69 7924.5 70 5890.5 71 4521.5 72 4002.5 73 3445.5 74 2541.0 75 1824.5 76 1365.0 77 1157.0 78 1063.5 79 893.5 80 629.0 81 415.0 82 293.0 83 228.5 84 162.0 85 112.5 86 99.5 87 96.0 88 88.0 89 90.5 90 75.0 91 60.0 92 51.0 93 44.0 94 39.0 95 26.0 96 20.0 97 13.0 98 6.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15832491453029793 2 0.0 3 0.0 4 0.08306394562002911 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.09795601380062E-4 12-13 6.776778780188856E-5 14-15 0.0 16-17 4.162878393544582E-4 18-19 0.0 20-21 0.0013747179811240248 22-23 3.2270375143756452E-6 24-25 0.0 26-27 6.583156529326316E-4 28-29 3.2270375143756452E-6 30-31 7.09948253162642E-4 32-33 0.0 34-35 0.0 36-37 0.0012262742554627452 38-39 0.0051793952105729105 40-41 0.005915159763850558 42-43 0.0016877406200184624 44-45 0.003139907501487503 46-47 0.019278322110880104 48-49 0.009787604781101333 50-51 0.009126062090654325 52-53 0.012311148117343088 54-55 0.01104937644922221 56-57 0.015518823406632479 58-59 0.022563446300514513 60-61 0.01089447864853218 62-63 0.003920850579966409 64-65 0.014263505813540354 66-67 0.026987714732723524 68-69 0.03136680463973127 70-71 0.014547485114805409 72-73 0.013075956008250114 74-75 0.025577499338941365 76-77 0.042319369963522216 78-79 0.007764252259587803 80-81 0.002010444371456027 82-83 0.0025525866738711355 84-85 0.012988825995361974 86-87 0.014934729616530487 88-89 0.01848124384482932 90-91 0.009716609955785068 92-93 0.019397722498912005 94-95 0.027384640346991725 96-97 0.018161767130906132 98-99 0.008061139710910362 100-101 0.31681763501134336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5494087E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.4370178861309 #Duplication Level Percentage of deduplicated Percentage of total 1 76.31629709828694 16.35993825899257 2 8.324125943079192 3.568888734563898 3 3.434515868800188 2.208773343290101 4 1.8384278345735934 1.5764164148846005 5 1.3708354811165864 1.469331236381956 6 0.9475818267631242 1.218799714133621 7 0.7646427164994072 1.1474161713068258 8 0.64135835135246 1.099904835948969 9 0.48967173461795455 0.9447391560004051 >10 4.4120948288030215 20.367222270698694 >50 0.7251698261359394 10.963632731074311 >100 0.6696213139982852 27.964045125863862 >500 0.0557845712650761 8.050194255505634 >1k 0.009781983848379078 2.7002287888951546 >5k 3.0320762814571604E-5 0.062080233038561215 >10k+ 6.030009713560696E-5 0.2983887294210144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27473 0.17731280326488422 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18694 0.12065247858747663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.419896506325284E-4 0.0 0.0 2 1.4844372566127968E-4 0.0 0.0 3 1.6135187571878227E-4 0.0 0.0 4 1.6135187571878227E-4 0.0 0.0 5 1.6135187571878227E-4 0.0 0.0 6 1.6135187571878227E-4 0.0 0.0 7 1.7426002577628486E-4 6.4540750287512905E-6 0.0 8 1.7426002577628486E-4 6.4540750287512905E-6 0.0 9 2.065304009200413E-4 6.4540750287512905E-6 0.0 10-11 2.54935963635676E-4 6.4540750287512905E-6 0.0 12-13 3.2270375143756455E-4 6.4540750287512905E-6 0.0 14-15 4.905097021850981E-4 6.4540750287512905E-6 6.4540750287512905E-6 16-17 7.389915907920228E-4 6.4540750287512905E-6 6.4540750287512905E-6 18-19 0.0010616953422295872 6.4540750287512905E-6 6.4540750287512905E-6 20-21 0.0014360316938971623 6.4540750287512905E-6 6.4540750287512905E-6 22-23 0.0018490924957372448 6.4540750287512905E-6 6.4540750287512905E-6 24-25 0.002300877747749835 6.4540750287512905E-6 6.4540750287512905E-6 26-27 0.0028752904253086997 6.4540750287512905E-6 6.4540750287512905E-6 28-29 0.0033948434651231785 6.4540750287512905E-6 6.4540750287512905E-6 30-31 0.004140289130943953 6.4540750287512905E-6 1.2908150057502581E-5 32-33 0.004888961834279103 6.4540750287512905E-6 1.2908150057502581E-5 34-35 0.005640861575128628 6.4540750287512905E-6 1.2908150057502581E-5 36-37 0.006621880979498824 6.4540750287512905E-6 1.2908150057502581E-5 38-39 0.007696484471785914 6.4540750287512905E-6 1.2908150057502581E-5 40-41 0.009074429490424314 6.4540750287512905E-6 1.2908150057502581E-5 42-43 0.010620180459810249 6.4540750287512905E-6 1.2908150057502581E-5 44-45 0.012411186280288733 6.4540750287512905E-6 1.2908150057502581E-5 46-47 0.014411949539201632 6.4540750287512905E-6 1.2908150057502581E-5 48-49 0.01690322250029963 6.4540750287512905E-6 1.2908150057502581E-5 50-51 0.019930183688783985 6.4540750287512905E-6 1.2908150057502581E-5 52-53 0.02359609830511472 6.4540750287512905E-6 1.2908150057502581E-5 54-55 0.027810609298889313 6.4540750287512905E-6 1.2908150057502581E-5 56-57 0.033138448235123505 6.4540750287512905E-6 1.2908150057502581E-5 58-59 0.03971837772693544 6.4540750287512905E-6 1.2908150057502581E-5 60-61 0.048060269701596485 9.681112543126935E-6 1.2908150057502581E-5 62-63 0.05813508082147725 1.2908150057502581E-5 1.2908150057502581E-5 64-65 0.07069471082742726 1.2908150057502581E-5 1.2908150057502581E-5 66-67 0.08672017912381672 1.6135187571878227E-5 1.2908150057502581E-5 68-69 0.10650837316196818 1.936222508625387E-5 1.2908150057502581E-5 70-71 0.1306304785819261 1.936222508625387E-5 1.2908150057502581E-5 72-73 0.16076455489116592 1.936222508625387E-5 1.2908150057502581E-5 74-75 0.19830145525838339 1.936222508625387E-5 1.2908150057502581E-5 76-77 0.24521289960486217 1.936222508625387E-5 1.2908150057502581E-5 78-79 0.3038223549409526 1.936222508625387E-5 1.2908150057502581E-5 80-81 0.37693734390416167 1.936222508625387E-5 1.2908150057502581E-5 82-83 0.46700073389287156 1.936222508625387E-5 1.2908150057502581E-5 84-85 0.5764747545305509 1.936222508625387E-5 1.2908150057502581E-5 86-87 0.708015257691531 1.936222508625387E-5 1.2908150057502581E-5 88-89 0.866872633411701 1.936222508625387E-5 1.2908150057502581E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 130970 0.0 66.75533 1 TTTGTGT 139570 0.0 62.67668 2 ACTTTGT 36545 0.0 54.534447 1 TTGTGTT 166870 0.0 53.994835 3 TGTGTTT 173820 0.0 52.158535 4 AACTTTG 17790 0.0 48.859924 2 TAACTTT 18385 0.0 47.093933 1 GTGTTTG 202025 0.0 44.93242 5 TTGAGTC 17955 0.0 35.38841 4 GTTTGAA 20535 0.0 33.67312 2 TTTGAGG 55500 0.0 33.485 8 TTTGAGT 56855 0.0 31.947514 3 GTTTGAG 148580 0.0 31.943932 7 TGTTTGA 286570 0.0 31.771284 1 TTGAGGG 31825 0.0 30.122458 9 GTTTGAC 56915 0.0 29.841087 7 TTTGACC 15600 0.0 28.488771 8 TTGAGGT 14990 0.0 28.444424 9 TTTGACA 7415 0.0 28.43114 8 TGAGTCC 12500 0.0 27.6531 5 >>END_MODULE