FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_7153.34100000035ccc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_7153.34100000035ccc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15494087
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274730.17731280326488422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186940.12065247858747663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1309700.066.755331
TTTGTGT1395700.062.676682
ACTTTGT365450.054.5344471
TTGTGTT1668700.053.9948353
TGTGTTT1738200.052.1585354
AACTTTG177900.048.8599242
TAACTTT183850.047.0939331
GTGTTTG2020250.044.932425
TTGAGTC179550.035.388414
GTTTGAA205350.033.673122
TTTGAGG555000.033.4858
TTTGAGT568550.031.9475143
GTTTGAG1485800.031.9439327
TGTTTGA2865700.031.7712841
TTGAGGG318250.030.1224589
GTTTGAC569150.029.8410877
TTTGACC156000.028.4887718
TTGAGGT149900.028.4444249
TTTGACA74150.028.431148
TGAGTCC125000.027.65315
Produced by FastQC (version 0.11.2)