FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_7098.34100000035c89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_7098.34100000035c89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20351526
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG2746300.065.8361051
TAACTTT407350.061.996221
TTTGTGT2952350.061.3322752
AACTTTG434900.058.586962
TTGTGTT3325350.055.9917263
TGTGTTT3583100.052.3512154
GTGTTTG3892100.048.189785
ACTTTGT818950.048.080761
TTTGAGG981000.039.0254148
GTTTGAG2585600.038.166977
TGTTTGA4977450.037.6560756
TTGAGGG502650.037.491719
GTTTGAC1032850.034.7861487
TTTGAGA342350.033.3136758
TTTGATG364100.033.0710838
TTTGAGC658600.032.6729288
TTTGACA126000.031.88018
GTTTGAA329500.031.8605447
TTGAGGC182250.030.585849
TTGACCC172350.030.1663429