##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l07n01_7047.34100000035c46.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18850123 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74383488107743 33.0 33.0 33.0 33.0 33.0 2 32.770836349449816 33.0 33.0 33.0 33.0 33.0 3 32.7968687525275 33.0 33.0 33.0 33.0 33.0 4 36.716001800094354 37.0 37.0 37.0 37.0 37.0 5 36.7265074609858 37.0 37.0 37.0 37.0 37.0 6 36.67556954402897 37.0 37.0 37.0 37.0 37.0 7 36.63842973332323 37.0 37.0 37.0 37.0 37.0 8 36.62926767109159 37.0 37.0 37.0 37.0 37.0 9 36.619475268145464 37.0 37.0 37.0 37.0 37.0 10-11 36.6117931962566 37.0 37.0 37.0 37.0 37.0 12-13 36.58135811633696 37.0 37.0 37.0 37.0 37.0 14-15 38.688351025614 40.0 37.0 40.0 37.0 40.0 16-17 38.650720899805265 40.0 37.0 40.0 37.0 40.0 18-19 38.60898573446974 40.0 37.0 40.0 37.0 40.0 20-21 38.55783275260326 40.0 37.0 40.0 37.0 40.0 22-23 38.508706547962575 40.0 37.0 40.0 37.0 40.0 24-25 38.46987162895435 40.0 37.0 40.0 37.0 40.0 26-27 38.38345115838236 40.0 37.0 40.0 37.0 40.0 28-29 38.31809999329978 40.0 37.0 40.0 37.0 40.0 30-31 38.269105246687246 40.0 37.0 40.0 37.0 40.0 32-33 38.19030106593999 40.0 37.0 40.0 37.0 40.0 34-35 38.11165351547043 40.0 37.0 40.0 37.0 40.0 36-37 38.01562416860622 40.0 37.0 40.0 37.0 40.0 38-39 37.89345528408488 40.0 37.0 40.0 33.0 40.0 40-41 37.766355635981796 40.0 37.0 40.0 33.0 40.0 42-43 37.63923620020941 40.0 37.0 40.0 33.0 40.0 44-45 37.486564543902446 40.0 37.0 40.0 33.0 40.0 46-47 37.2974722233908 38.5 37.0 40.0 33.0 40.0 48-49 37.068962759553344 37.0 37.0 40.0 33.0 40.0 50-51 36.8601656604575 37.0 37.0 40.0 33.0 40.0 52-53 36.62015690825996 37.0 37.0 40.0 33.0 40.0 54-55 36.334944764020904 37.0 37.0 40.0 33.0 40.0 56-57 36.09485553489492 37.0 37.0 40.0 33.0 40.0 58-59 35.82156641630402 37.0 35.0 38.5 33.0 40.0 60-61 35.53390946043164 37.0 33.0 37.0 33.0 40.0 62-63 35.28062090098828 37.0 33.0 37.0 33.0 40.0 64-65 35.00352541466175 37.0 33.0 37.0 30.0 40.0 66-67 34.76488755537564 37.0 33.0 37.0 27.0 38.5 68-69 34.51872929423325 37.0 33.0 37.0 27.0 37.0 70-71 34.27526303144016 37.0 33.0 37.0 27.0 37.0 72-73 34.065514851017156 37.0 33.0 37.0 27.0 37.0 74-75 33.80195391828478 37.0 33.0 37.0 27.0 37.0 76-77 30.98406583341658 33.0 30.0 33.0 24.5 37.0 78-79 33.58442902468063 35.0 33.0 37.0 27.0 37.0 80-81 34.08826019331545 37.0 33.0 37.0 27.0 37.0 82-83 34.05236369014675 37.0 33.0 37.0 27.0 37.0 84-85 33.97917538787413 37.0 33.0 37.0 27.0 37.0 86-87 33.82279306612482 37.0 33.0 37.0 27.0 37.0 88-89 33.68276053158911 37.0 33.0 37.0 27.0 37.0 90-91 33.59609982385791 37.0 33.0 37.0 27.0 37.0 92-93 33.501130470077044 37.0 33.0 37.0 27.0 37.0 94-95 33.402437798416486 37.0 33.0 37.0 27.0 37.0 96-97 33.282983325891294 37.0 33.0 37.0 27.0 37.0 98-99 33.10379717416167 33.0 33.0 37.0 27.0 37.0 100-101 31.084354515883 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1172668609721228 2306 2 0.10816186338312406 2306 3 0.0914189099066931 2306 4 0.06709385606245633 2306 5 0.06433167909392523 2306 6 0.08046230821911138 2306 7 0.11607435035396207 2306 8 0.12717656745253691 2306 9 0.0947547691518551 2306 10-11 0.09019424641261509 2306 12-13 0.10303046731059595 2306 14-15 0.18626966654758803 2306 16-17 0.1536773240616114 2306 18-19 0.18450427880222975 2306 20-21 0.16775065962792013 2306 22-23 0.12487374473096935 2306 24-25 0.10382345179593955 2306 26-27 0.13278858338911448 2306 28-29 0.11184347563820296 2306 30-31 0.08440447852995447 2306 32-33 0.08980990296108615 2306 34-35 0.08159569444120507 2306 36-37 0.08288073149433473 2306 38-39 0.05977977006376989 2306 40-41 0.0657380880829379 2306 42-43 0.10353037996945602 2306 44-45 0.07101671507557938 2306 46-47 0.051252994241970384 2306 48-49 0.006163491490561057 2306 50-51 0.03218659264042145 2306 52-53 0.04064156775281447 2306 54-55 0.04128294769481755 2306 56-57 0.07305420025635811 2306 58-59 -0.048800990601741034 2306 60-61 -0.17165689352429325 2306 62-63 -0.20561103113539048 2306 64-65 -0.17864920153038355 2306 66-67 -0.20876702473271536 2306 68-69 -0.2570854431778642 2306 70-71 -0.20373544802789212 2306 72-73 -0.3088579730497045 2306 74-75 -0.35926449793880266 2306 76-77 -0.24807182231941383 2306 78-79 -0.1906080259577081 2306 80-81 -0.13984025261434851 2306 82-83 -0.19497605050089106 2306 84-85 -0.2955704991797319 2306 86-87 -0.20332256390947379 2306 88-89 -0.34752571936543575 2306 90-91 -0.39755942114008036 2306 92-93 -0.3942074784921914 2306 94-95 -0.42330944559650163 2306 96-97 -0.37964659369733056 2306 98-99 -0.36048176424434786 2306 100-101 -0.695924614561747 2307 1 0.09643794266767713 2307 2 0.0968244220683161 2307 3 0.08083084560147569 2307 4 0.04951042003160211 2307 5 0.07336547847820896 2307 6 0.07909096916106506 2307 7 0.10419070233317029 2307 8 0.11050358683247197 2307 9 0.0905450143237232 2307 10-11 0.09012443938689785 2307 12-13 0.0900895261513881 2307 14-15 0.1633089773800549 2307 16-17 0.13988824367097408 2307 18-19 0.1745552401490187 2307 20-21 0.198425846811368 2307 22-23 0.12133306463632465 2307 24-25 0.10681698756857116 2307 26-27 0.12870702990977634 2307 28-29 0.11456083812148421 2307 30-31 0.06357986519373782 2307 32-33 0.055261045886801696 2307 34-35 0.04347290718983743 2307 36-37 0.0572621630109964 2307 38-39 0.04651072882636953 2307 40-41 0.014905698784438925 2307 42-43 0.0426890101241284 2307 44-45 -0.03015157062881002 2307 46-47 -0.008590142192936412 2307 48-49 -0.04801157456902416 2307 50-51 -0.007231768244643888 2307 52-53 -0.10512351200645043 2307 54-55 -0.048884505890463004 2307 56-57 -0.07113297254336715 2307 58-59 -0.09315879842884556 2307 60-61 -0.20119345189919358 2307 62-63 -0.20213126079606525 2307 64-65 -0.20898122628779703 2307 66-67 -0.2500626088515716 2307 68-69 -0.2663389874975195 2307 70-71 -0.38375466088935895 2307 72-73 -0.4853899348073938 2307 74-75 -0.516972789541498 2307 76-77 -0.8194954190540287 2307 78-79 -0.3196420231487309 2307 80-81 -0.25746029196518094 2307 82-83 -0.23847135744028236 2307 84-85 -0.3178715956339673 2307 86-87 -0.2818910569217721 2307 88-89 -0.3707875345069951 2307 90-91 -0.36638139955345395 2307 92-93 -0.35440663579920084 2307 94-95 -0.42337683296794637 2307 96-97 -0.39685785161469056 2307 98-99 -0.3466417501313188 2307 100-101 -0.6894571206609932 2201 1 0.08106366991430747 2201 2 0.07684252847901973 2201 3 0.07049553635454231 2201 4 0.07714281919696475 2201 5 0.07749345337665403 2201 6 0.05904328359641653 2201 7 0.10069049586480361 2201 8 0.09671801130466662 2201 9 0.1038743358847043 2201 10-11 0.08116227127323583 2201 12-13 0.09385032989742825 2201 14-15 0.11959939156026422 2201 16-17 0.14669674413907074 2201 18-19 0.13879631374385326 2201 20-21 0.1322782975043566 2201 22-23 0.17841488238351388 2201 24-25 0.1411722993070086 2201 26-27 0.0791257137282031 2201 28-29 0.11099685290186301 2201 30-31 0.033588362504637814 2201 32-33 0.09082307615293672 2201 34-35 0.05222486977758223 2201 36-37 0.020916137184038064 2201 38-39 0.028006278312268762 2201 40-41 0.09313529265561016 2201 42-43 0.13414165272441636 2201 44-45 0.07583255643505282 2201 46-47 0.020410734293342614 2201 48-49 0.05553005304438585 2201 50-51 -0.04199181563932797 2201 52-53 -0.004794609045568166 2201 54-55 -7.290392127927703E-4 2201 56-57 -0.10590287909158036 2201 58-59 -0.07929037026259067 2201 60-61 -0.09466043904102861 2201 62-63 -0.10900654385866915 2201 64-65 -0.17009232438507382 2201 66-67 -0.16809153207076122 2201 68-69 -0.2448120714157227 2201 70-71 -0.24786927343619425 2201 72-73 -0.23444097481732484 2201 74-75 -0.4033460192942684 2201 76-77 -0.5200339743745737 2201 78-79 -0.2139224586614077 2201 80-81 -0.13604695182677062 2201 82-83 -0.1218360448574245 2201 84-85 -0.17011314380405196 2201 86-87 -0.13838098302121438 2201 88-89 -0.06766611662299482 2201 90-91 -0.11767727615629298 2201 92-93 -0.12920679085132747 2201 94-95 -0.22771665830927645 2201 96-97 -0.3028310409449304 2201 98-99 -0.22798583319701748 2201 100-101 -0.39911096672148716 1101 1 0.08310342053177067 1101 2 0.06539135127937357 1101 3 0.06245576320951329 1101 4 0.03834728050734526 1101 5 0.06757017985612634 1101 6 0.08434790770485989 1101 7 0.06123335884017678 1101 8 0.06710438804130092 1101 9 0.08818952495815324 1101 10-11 0.04956043813296418 1101 12-13 0.05974645455126648 1101 14-15 0.10616647685713332 1101 16-17 0.10135944261873675 1101 18-19 0.06134656970505148 1101 20-21 0.08850292925840364 1101 22-23 0.1339267721401214 1101 24-25 0.07846554982731391 1101 26-27 0.07798657600479686 1101 28-29 0.07394312387700808 1101 30-31 0.046267775140854894 1101 32-33 0.018372261128241973 1101 34-35 0.023614471013594596 1101 36-37 0.007555861305810652 1101 38-39 -0.03406762790658746 1101 40-41 -0.015574744392701234 1101 42-43 -0.05110914373819497 1101 44-45 -0.0681124396148789 1101 46-47 -0.04046805942250131 1101 48-49 -0.06149864496966728 1101 50-51 -0.0456592622865557 1101 52-53 -0.02869387440205884 1101 54-55 -0.04708843306138988 1101 56-57 -0.09960013081990127 1101 58-59 -0.12321561564661465 1101 60-61 -0.18549871106662863 1101 62-63 -0.13704824071485433 1101 64-65 -0.09274987085549213 1101 66-67 -0.2358561766660472 1101 68-69 -0.2021732731213035 1101 70-71 -0.25869757403592786 1101 72-73 -0.26817879045852777 1101 74-75 -0.3525626721737112 1101 76-77 -0.841064708975857 1101 78-79 -0.25685911187493815 1101 80-81 -0.05296356924582568 1101 82-83 -0.06430465282050335 1101 84-85 -0.05584176290411591 1101 86-87 -0.019544308890935724 1101 88-89 -0.15243240232893385 1101 90-91 -0.17572261302763792 1101 92-93 -0.09963065838087459 1101 94-95 -0.06739884730249912 1101 96-97 -0.29274573969571804 1101 98-99 -0.15180675239236763 1101 100-101 -0.30632648244860405 2304 1 0.09748984608177125 2304 2 0.07408820779186698 2304 3 0.0513557400942517 2304 4 0.02752501907724536 2304 5 0.045883041299788374 2304 6 0.05485068524698988 2304 7 0.08048950564167257 2304 8 0.0793589785438158 2304 9 0.05362690438491313 2304 10-11 0.07806985108758369 2304 12-13 0.07250071678921444 2304 14-15 0.11894230431438757 2304 16-17 0.08749808968520512 2304 18-19 0.0694468894946283 2304 20-21 0.11649537886624017 2304 22-23 0.14321289463797626 2304 24-25 0.12354550917726925 2304 26-27 0.09936544815719373 2304 28-29 0.0673735963396993 2304 30-31 0.05614578299911699 2304 32-33 0.10449024958635533 2304 34-35 0.07908943270222579 2304 36-37 0.102264501203571 2304 38-39 0.07793041286729618 2304 40-41 0.0859064972538377 2304 42-43 0.07457959000509362 2304 44-45 -0.007796339411520137 2304 46-47 0.058045372176501076 2304 48-49 0.06203107407774411 2304 50-51 0.02395207841354363 2304 52-53 -0.019618059492898965 2304 54-55 0.028384164049718663 2304 56-57 -0.034758906625455666 2304 58-59 -0.021738973473219403 2304 60-61 -0.06889147723112643 2304 62-63 -0.11852217310116941 2304 64-65 -0.1287588887452742 2304 66-67 -0.12475165827149226 2304 68-69 -0.17129648684658605 2304 70-71 -0.18128473608610562 2304 72-73 -0.16782376385123854 2304 74-75 -0.30365162774445054 2304 76-77 -0.8047993617834628 2304 78-79 -0.19945205597760918 2304 80-81 -0.04341103845546712 2304 82-83 -0.05070945703496221 2304 84-85 -0.07004823199169863 2304 86-87 -0.01969358005957389 2304 88-89 -0.13064331362753023 2304 90-91 -0.14636644453887726 2304 92-93 -0.11723487409855693 2304 94-95 -0.06281281765257063 2304 96-97 -0.15401341485249276 2304 98-99 -0.13549155977963068 2304 100-101 -0.1886863119685529 2202 1 0.07989280673307775 2202 2 0.0543831025849002 2202 3 0.05928478641951074 2202 4 0.015946757599799355 2202 5 0.0220872334447364 2202 6 0.025730891825517688 2202 7 0.02202168651486147 2202 8 0.06098584781096861 2202 9 0.0578131796682797 2202 10-11 0.04844072583254189 2202 12-13 0.05198973216226932 2202 14-15 0.06236047648033605 2202 16-17 0.06254591078780436 2202 18-19 0.03303182768192414 2202 20-21 0.0496427230491534 2202 22-23 0.052558376820655894 2202 24-25 0.06530163088733332 2202 26-27 0.09782168999181806 2202 28-29 0.02355127617521191 2202 30-31 0.02415629740384162 2202 32-33 -0.023052507022512714 2202 34-35 -0.028186023508681046 2202 36-37 0.030190649355994026 2202 38-39 0.06687195388511213 2202 40-41 0.038673887662746154 2202 42-43 0.07014747304398128 2202 44-45 0.029559905517011487 2202 46-47 0.03467350104979516 2202 48-49 0.06399779186605059 2202 50-51 0.003352538722431575 2202 52-53 0.011159041514737567 2202 54-55 0.04791028699437305 2202 56-57 0.02648640696198612 2202 58-59 -0.02590384819368552 2202 60-61 -0.07865951803267279 2202 62-63 -0.13319228925743687 2202 64-65 -0.04786415128028665 2202 66-67 -0.10178274541976862 2202 68-69 -0.22243404939129618 2202 70-71 -0.12902437584125437 2202 72-73 -0.15823587197588296 2202 74-75 -0.27436374678609354 2202 76-77 -0.5123952847305873 2202 78-79 -0.1369616912141467 2202 80-81 0.03773447702384658 2202 82-83 0.03897217059566316 2202 84-85 -0.010776892177396746 2202 86-87 0.07372391634026343 2202 88-89 0.010638991295053302 2202 90-91 -0.08274261012989115 2202 92-93 -0.03318081673396023 2202 94-95 0.005367478199005404 2202 96-97 0.006743808782339045 2202 98-99 -0.10499578110174212 2202 100-101 0.08786466544250615 1102 1 0.07549124491293213 1102 2 0.059370895579831995 1102 3 0.04945930629961026 1102 4 0.02423475015888954 1102 5 0.03183415266524747 1102 6 0.04728717266750948 1102 7 0.03782202131979773 1102 8 0.06366471467705992 1102 9 0.0806315983907453 1102 10-11 0.042643734423990054 1102 12-13 0.04479744426376442 1102 14-15 0.06088854810675315 1102 16-17 0.024679378601369706 1102 18-19 0.0882873120916372 1102 20-21 0.07552708443187583 1102 22-23 0.06564242951126431 1102 24-25 0.06345636867788329 1102 26-27 0.08326557765245468 1102 28-29 0.040462700361977966 1102 30-31 0.03327686629736348 1102 32-33 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-0.6301589302110884 1213 52-53 -0.2604337839051283 1213 54-55 -0.837951215851362 1213 56-57 -0.3331324623479972 1213 58-59 -0.12986450015048234 1213 60-61 -0.14289499952666063 1213 62-63 -0.007088213109831543 1213 64-65 -0.04371344106170483 1213 66-67 -0.34918182419415444 1213 68-69 -0.08066742706563446 1213 70-71 -0.042912462708216026 1213 72-73 0.03243457578802378 1213 74-75 0.09292567221287129 1213 76-77 0.6285721095339625 1213 78-79 -0.038220115958537804 1213 80-81 -0.18290196491059874 1213 82-83 -0.12214533675248873 1213 84-85 -0.09428678498081666 1213 86-87 -1.4010932256507616 1213 88-89 -0.6729233564341826 1213 90-91 -0.36712131250286717 1213 92-93 -0.28308576292930354 1213 94-95 -0.010315390187912499 1213 96-97 0.07866575080431915 1213 98-99 0.015806396745198015 1213 100-101 0.45021400071337325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 42.0 8 419.0 9 1500.0 10 1002.0 11 392.0 12 292.0 13 538.0 14 1283.0 15 2603.0 16 4924.0 17 7754.0 18 11085.0 19 15368.0 20 20446.0 21 27054.0 22 34572.0 23 45337.0 24 58829.0 25 75773.0 26 97254.0 27 125655.0 28 162284.0 29 210821.0 30 273957.0 31 364766.0 32 501405.0 33 730348.0 34 1179374.0 35 2264066.0 36 4912881.0 37 6562980.0 38 1154975.0 39 142.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.55401540766617 22.729017487901952 30.560969718249464 26.15599738618241 2 27.20339808923263 23.025170711087668 29.72183788933367 20.04959331034604 3 21.538289166601192 21.091602426148626 36.47543838308111 20.894670024169073 4 21.516445340013572 21.279862957535165 35.447027028232405 21.756664674218854 5 28.10878210184623 20.50586089013849 31.13907002092241 20.24628698709287 6 29.179035065182333 21.08341680316887 30.068201676986405 19.669346454662392 7 28.146590873704113 26.658908273436733 24.989757361264964 20.204743491594193 8 26.39882508989464 22.619794045906225 29.943162705092163 21.03821815910697 9 22.94501208294503 21.706028125121517 33.57384988946757 21.77510990246589 10-11 27.403567436163613 22.492363951655552 27.820022255627354 22.284046356553485 12-13 26.37431739465746 25.94743930573359 24.742186551937024 22.93605674767193 14-15 26.077962462101706 24.159128829026738 26.02436864735578 23.738540061515778 16-17 25.6617447535299 23.907373214560913 25.55490156394769 24.8759804679615 18-19 25.498162531883743 24.481673143459066 26.024092787086854 23.996071537570337 20-21 24.60635491596537 24.866784071680577 26.367443387341904 24.159417625012146 22-23 24.715458075019356 24.967663339261144 25.856848565754643 24.460030019964858 24-25 25.1422974799687 24.818058216384053 25.57746970669634 24.462174596950906 26-27 24.90097741680859 24.902889884362292 25.825243012026583 24.370889686802535 28-29 24.852046050418135 25.352912835259104 25.308218164317452 24.486822950005312 30-31 24.99976326232945 25.215620272458132 25.2706203351903 24.51399613002211 32-33 24.812143135617738 25.51645153721278 25.282105586260627 24.38929974090885 34-35 24.62530615847971 25.5706633850612 25.25742670220242 24.546603754256672 36-37 24.763793445031197 25.43089624206132 25.332245686488474 24.47306462641901 38-39 24.786696384134792 25.142528244495445 25.426428177813115 24.644347193556648 40-41 24.66348917201115 25.43160594751282 25.065634705079127 24.839270175396898 42-43 24.63233022868751 25.33596247358355 25.183132953698113 24.848574344030823 44-45 24.91731062852729 25.145428569306493 25.220945321510534 24.71631548065568 46-47 24.636456878584255 25.202700049664866 25.371534071373087 24.789309000377795 48-49 24.669264424598317 25.030487697311194 25.279044898587404 25.021202979503084 50-51 24.586706868336435 25.21727020015858 25.6883231675209 24.507699763984082 52-53 24.732744178028764 25.094092953478693 25.71388945563889 24.45927341285366 54-55 24.494935708382457 25.220635435785127 25.324079062828435 24.96034979300398 56-57 24.658584842112756 25.416239644862177 25.490155504249714 24.43502000877535 58-59 24.570133777782377 25.57048637614578 25.452401741395498 24.406978104676348 60-61 24.987722854855388 25.450731455754262 25.101368684154256 24.46017700523609 62-63 24.63563933718981 25.624285186339367 25.5128567516342 24.22721872483662 64-65 24.85482689402057 25.515323737949686 25.278680101427646 24.351169266602103 66-67 25.079815910138574 25.462154881257966 25.376359908425222 24.081669300178238 68-69 24.85859663223704 25.1977020337515 25.753869597982735 24.189831736028722 70-71 24.734999730599252 25.60237463162333 25.43058158222627 24.23204405555115 72-73 24.607639199863154 25.23984251343515 25.723244198617373 24.429274088084323 74-75 24.592777545594036 25.214117865806664 25.705372203509558 24.487732385089743 76-77 24.483139665282422 25.244965591294445 25.536728147290255 24.735166596132878 78-79 24.737744797982767 25.087625312838597 25.369670469827234 24.80495941935141 80-81 24.56900091524042 25.038616015582644 25.72553174874157 24.666851320435367 82-83 24.380692428486547 25.660021963330788 25.579688693659282 24.379596914523386 84-85 24.510839939739853 25.377175063114088 25.460740017277516 24.65124497986854 86-87 24.40385500486689 25.511369980733782 25.654791514693702 24.42998349970562 88-89 24.445438966543147 26.34262207776448 24.972553365537596 24.23938559015477 90-91 24.525985774612572 25.535412420256627 25.18713649854623 24.75146530658457 92-93 24.321800932917277 25.804084081888362 25.230121956405867 24.643993028788493 94-95 24.42293964344441 25.981035970631293 24.858495440739564 24.737528945184735 96-97 24.296175343362393 25.42369769708998 25.224431334764148 25.055695624783482 98-99 24.545851417294163 25.40333960623315 25.17782876492897 24.87298021154371 100-101 24.739938926123344 25.965857876354352 24.64104354834795 24.65315964917435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3510.0 1 2367.0 2 1630.0 3 1904.5 4 1924.5 5 1856.0 6 1634.0 7 1644.0 8 1752.5 9 1855.5 10 1874.0 11 1991.0 12 2155.0 13 2291.0 14 2447.5 15 2622.5 16 2849.5 17 3144.0 18 3439.0 19 3882.5 20 4495.0 21 5136.0 22 6047.0 23 7070.5 24 8381.0 25 10260.5 26 12469.5 27 14774.0 28 17290.0 29 20243.0 30 24334.0 31 31007.0 32 41636.5 33 55337.5 34 73154.0 35 102080.0 36 134108.0 37 171364.5 38 217029.5 39 265165.0 40 328918.5 41 416993.0 42 507174.5 43 592223.0 44 705082.0 45 838100.5 46 965127.0 47 1097743.0 48 1212470.5 49 1238890.5 50 1178844.5 51 1108524.0 52 1052691.0 53 1021214.0 54 978593.0 55 900155.0 56 821093.0 57 679906.5 58 514422.5 59 394363.5 60 303177.5 61 235164.5 62 169328.5 63 112200.5 64 71803.0 65 44601.5 66 27114.5 67 17254.5 68 12437.5 69 9615.0 70 7383.5 71 5323.5 72 3942.0 73 3099.0 74 2328.0 75 1582.5 76 1133.0 77 924.5 78 730.0 79 585.0 80 412.0 81 273.5 82 194.0 83 117.0 84 77.5 85 58.5 86 47.5 87 47.5 88 48.0 89 35.5 90 28.5 91 30.5 92 29.5 93 23.0 94 11.5 95 10.0 96 12.0 97 6.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15989816087672212 2 0.0 3 0.0 4 0.08312412603355426 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.129953051234732E-4 12-13 5.5702554301635065E-5 14-15 0.0 16-17 3.607403516677318E-4 18-19 0.0 20-21 0.0014588764221856803 22-23 5.305005171584292E-6 24-25 0.0 26-27 6.89650672305958E-4 28-29 5.305005171584292E-6 30-31 7.6657324729393E-4 32-33 0.0 34-35 0.0 36-37 0.0013501238161682022 38-39 0.0055808654405066745 40-41 0.005840810693914305 42-43 0.0019177593695277216 44-45 0.0035172184287603856 46-47 0.02017493466753506 48-49 0.010326192566488823 50-51 0.009644499401940242 52-53 0.012755884935074428 54-55 0.011543691253367417 56-57 0.016007853105255602 58-59 0.02290701233090097 60-61 0.011103375824125922 62-63 0.004270529163125355 64-65 0.01483279445974968 66-67 0.02756480687155198 68-69 0.03173454093641723 70-71 0.014517146652040413 72-73 0.01351184817202519 74-75 0.02596800031490511 76-77 0.042912186832945336 78-79 0.008198885492683522 80-81 0.0022864572289528298 82-83 0.0027745177047385845 84-85 0.013310257975504987 86-87 0.015296982412263304 88-89 0.018832768359124237 90-91 0.009808954562259355 92-93 0.019933556932227973 94-95 0.02777435457582956 96-97 0.018514468048829176 98-99 0.008400475689203725 100-101 0.32400319085451057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.8850123E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.062866002466475 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97349978599387 16.541302795143316 2 10.804154361501435 4.767412198955399 3 4.400713672147969 2.9127706819146852 4 1.9967417134268135 1.762153794594844 5 1.0438656906611774 1.1515334428814838 6 0.9677467503852785 1.281076012684366 7 0.7307310836266933 1.1285415387325 8 0.3663996062133734 0.6467060332193713 9 0.39091435760702403 0.7762221981291629 >10 3.039126171972418 13.931781924705206 >50 0.6091925506441735 9.486188910057358 >100 0.5791778407319027 25.45054656759754 >500 0.07025909201672721 10.781018093839894 >1k 0.027247273424913974 9.04887294243962 >5k 2.3004964628044736E-4 0.3338728651051202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0610010343168583E-4 0.0 0.0 2 1.1140510860327012E-4 0.0 0.0 3 1.1140510860327012E-4 0.0 0.0 4 1.1671011377485442E-4 0.0 0.0 5 1.2201511894643871E-4 0.0 5.305005171584291E-6 6 1.2201511894643871E-4 0.0 5.305005171584291E-6 7 1.379301344611916E-4 0.0 5.305005171584291E-6 8 1.5384514997594445E-4 0.0 5.305005171584291E-6 9 1.5915015514752874E-4 0.0 5.305005171584291E-6 10-11 1.8832768359124233E-4 0.0 5.305005171584291E-6 12-13 2.4137773530708527E-4 0.0 5.305005171584291E-6 14-15 3.103428025376811E-4 0.0 5.305005171584291E-6 16-17 3.925703826972376E-4 0.0 5.305005171584291E-6 18-19 4.774504654425862E-4 0.0 5.305005171584291E-6 20-21 6.047705895606092E-4 0.0 5.305005171584291E-6 22-23 7.612682421223459E-4 0.0 5.305005171584291E-6 24-25 9.46943423127796E-4 0.0 5.305005171584291E-6 26-27 0.0011405761118906226 0.0 5.305005171584291E-6 28-29 0.0013209462877244887 0.0 5.305005171584291E-6 30-31 0.0015198839816588997 0.0 5.305005171584291E-6 32-33 0.0018037017583386592 0.0 5.305005171584291E-6 34-35 0.0021538320996632226 0.0 5.305005171584291E-6 36-37 0.0025543599901178366 0.0 5.305005171584291E-6 38-39 0.0030477254710751757 0.0 5.305005171584291E-6 40-41 0.003641886050292616 0.0 5.305005171584291E-6 42-43 0.004371324261385456 0.0 5.305005171584291E-6 44-45 0.0051856425552236456 0.0 5.305005171584291E-6 46-47 0.006188288532653076 0.0 5.305005171584291E-6 48-49 0.007265204582484687 0.0 5.305005171584291E-6 50-51 0.008686945968469277 0.0 5.305005171584291E-6 52-53 0.01042168265957734 0.0 5.305005171584291E-6 54-55 0.012294349485146596 0.0 5.305005171584291E-6 56-57 0.01492032704508082 0.0 1.0610010343168582E-5 58-59 0.018084762629930848 0.0 1.0610010343168582E-5 60-61 0.02188049383019941 0.0 1.0610010343168582E-5 62-63 0.026893723717346565 0.0 1.0610010343168582E-5 64-65 0.033474582632696884 0.0 1.0610010343168582E-5 66-67 0.04133925279957059 0.0 1.0610010343168582E-5 68-69 0.0506230118498431 0.0 1.0610010343168582E-5 70-71 0.06284839626775911 0.0 1.0610010343168582E-5 72-73 0.07806845610503443 0.0 1.0610010343168582E-5 74-75 0.09747416502268977 0.0 1.0610010343168582E-5 76-77 0.12189575633007806 0.0 1.0610010343168582E-5 78-79 0.15245523862098936 0.0 1.0610010343168582E-5 80-81 0.19077594347792848 0.0 1.5915015514752876E-5 82-83 0.23855282005321662 0.0 1.5915015514752876E-5 84-85 0.2981041556068361 0.0 1.5915015514752876E-5 86-87 0.37079598897046984 0.0 1.5915015514752876E-5 88-89 0.45980071323672533 0.0 1.5915015514752876E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 155260 0.0 68.6052 1 TTTGTGT 167315 0.0 63.69792 2 TTGTGTT 189860 0.0 57.56585 3 AACTTTG 19350 0.0 56.68435 2 TAACTTT 19360 0.0 56.651485 1 TGTGTTT 203935 0.0 53.85848 4 ACTTTGT 41645 0.0 51.403675 1 GTGTTTG 227910 0.0 48.208054 5 TTGAGTC 20410 0.0 37.115356 4 TTTGAGG 70475 0.0 34.35487 8 GTTTGAG 181665 0.0 33.620903 7 TTGAGGG 37075 0.0 32.69701 9 TGTTTGA 337010 0.0 32.58197 6 TTTGAGT 67360 0.0 32.580685 3 TTTGAGC 45955 0.0 30.615211 8 TTGAGGC 13085 0.0 28.885355 9 TGAGTCT 5000 0.0 27.540155 5 GTTTGAC 66805 0.0 27.307804 7 GTTTGAA 30285 0.0 25.970407 2 TTGAGTG 32780 0.0 25.443478 4 >>END_MODULE