FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l07n01_7047.34100000035c46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l07n01_7047.34100000035c46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18850123
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1552600.068.60521
TTTGTGT1673150.063.697922
TTGTGTT1898600.057.565853
AACTTTG193500.056.684352
TAACTTT193600.056.6514851
TGTGTTT2039350.053.858484
ACTTTGT416450.051.4036751
GTGTTTG2279100.048.2080545
TTGAGTC204100.037.1153564
TTTGAGG704750.034.354878
GTTTGAG1816650.033.6209037
TTGAGGG370750.032.697019
TGTTTGA3370100.032.581976
TTTGAGT673600.032.5806853
TTTGAGC459550.030.6152118
TTGAGGC130850.028.8853559
TGAGTCT50000.027.5401555
GTTTGAC668050.027.3078047
GTTTGAA302850.025.9704072
TTGAGTG327800.025.4434784